Tài liệu Báo cáo khoa học: Mapping the functional domain of the prion protein docx

... respectively, of that of HPRT. This low activity could be explained by the fact that PRTFDC1 has a Gly in the position of the proposed catalytic Asp of HPRT. In PRTFDC1, a water molecule at the position of ... strength [20]. In the present study, we report the structural and functional characterization of the human HPRT homolog PRTFDC1. To shed light on the poten...

Ngày tải lên: 15/02/2014, 01:20

... all-alpha domain, STAT protein DNA-binding domain and SH2 domain of stat6, and the zinc-finger domain, leucine-zipper domain and fork-head domain of foxp3. In addition, for stat6 and foxp3, there ... whether these cytokines are produced by CD4 + CD25 + Foxp3 + T reg or whether they induce the production of these cytokines from another population of cells [20]. To date,...

Ngày tải lên: 16/02/2014, 09:20

... Kessler Department of Clinical Medicine, University of Oxford, UK Introduction The genus Yersinia consists of three pathogenic species that are agents of a variety of diseases, one of which was historically the ... essential for Yersinia pathogenesis [19]. The Rho family of small G proteins represents a large group of the Ras superfamily of GTPases. More than 20 prot...

Ngày tải lên: 16/02/2014, 15:20

... histones Historically, the location of the globular domain of the linker histone has been a matter of contention [13]. Currently, although there is a good degree of agree- ment about the overall parameters of the ... respectively. Although the over- all number of these modifications is low, the density of modifications is much the same as in the rest of the...

Ngày tải lên: 18/02/2014, 08:20

... involved in the regulation of genes participating in cholesterol biosynthesis and low-density lipoprotein receptor synthesis [1,2]. They are now estab- lished as global regulators of lipid synthesis. ... functions. The gene encoding the cyclin-depen- dent kinase inhibitor p21 is a target gene of SREBP [20]. This finding suggests that the regulation of lipid synthesis is linked...

Ngày tải lên: 18/02/2014, 13:20

... centrifugation of the samples, proteins bound to liposomes were analyzed by SDS ⁄ PAGE of the resulting pellets. (C) The amounts of I C in the pellets. The amounts of I C were quantitated with the UN-SCAN-IT ... phospholipid and membrane binding of the other members of the PEBP family. Consequently, the binding characteristics of PEBP proteins, including those...

Ngày tải lên: 19/02/2014, 05:20

... retain functionality and phylogeny. How- ever, the nature of the connections between these factors needs to be understood at the level of the pro- tein domain. The global properties of the HTFN topology ... analysis of the topological modules of the Fig. 3 (labelled A–G) shows that they include structural and ⁄or functional features. Table 3 summarizes the main...

Ngày tải lên: 19/02/2014, 07:20

... polarized light, respectively. The bottom panel displays the result of deconvolution of the IR absorption spectrum of the peptide f or analysis of the sec ondary struc ture. The dominant pe ak at 1655 ... that the fusion peptides of gp41 of HIV-1 and HA2 do not span both leaflets of the membrane bilayer [ 15,22]. The I FP of ASLV-A is shown to penetrate more s...

Ngày tải lên: 19/02/2014, 16:20

... by the cons program from the emboss package. A hydrophobicity plot was generated to verify the structural similarities of the proteins. It was based on the multiple sequence alignment of the ... transferase activity of 0.11 lmolÆmin )1 Æmg )1 in the membrane fraction from bacteria overexpressing the protein. Partial purification of the Synechocystis sp. protein yie...

Ngày tải lên: 19/02/2014, 17:20

... located in the cytochrome domain and approaches one of propionates of heme, was mutated to Tyr, and the thermodynamic and kinetic properties of the mutant (F166Y) were compared with those of the wild-type ... because of the weaker domain interaction of this mutant enzyme. The F166Y mutant is, however, useful for monitoring the electron transfer step of CDH, bec...

Ngày tải lên: 19/02/2014, 18:20