Tài liệu Báo cáo khoa học: Interaction of the small heat shock protein with molecular mass 25 kDa (hsp25) with actin doc

... accumulation of cofilin. J. Biol. Chem. 264, 16143–16148. Ó FEBS 2003 Actin and small heat shock protein (Eur. J. Biochem. 270) 901 Interaction of the small heat shock protein with molecular mass 25 kDa (hsp25) ... this process. The data in the literature indicate that the small heat shock protein with molecular mass 25 27 kDa (H...

Ngày tải lên: 20/02/2014, 23:20

... GFP or b-Gal proteins for 30 min at 37 °C. These differences in the time courses of transduction may depend on whether the target protein is fused to the PEP-1 vector or mixed with the PEP-1 peptide. ... Fusion with the PEP-1 vector may alter the conformation, polarity or molecu- lar shape of the target protein, improving transduction of the fusion proteins into c...

Ngày tải lên: 18/02/2014, 17:20

... of the first 88 amino acids of PPI1 may participate in the interaction with the H + -ATPase and makes PPI1 588 His 6 a suitable tool to study the mechanism of action of PPI1. The analysis of the ... 14-3-3s. Results The C-terminus of isoform 1 of the PM H + -ATPase of A. thaliana (AHA1) interacts with the first 88 amino acids of PPI1 [22], indicating th...

Ngày tải lên: 19/02/2014, 07:20

... the functional changes with the structural modification of the actin HMM interface, which results in the inhibition of HMM cross- linking to the N-terminal part of a ctin. This change of the actin HMM ... between these two actin binding components in their functional a nd molecular effects on actin. Can the diminution of HMM contacts with the N-terminal pa...

Ngày tải lên: 19/02/2014, 16:20

... on the mica surface without being broken, resulting in ‘double membranes’, and these flatten on the mica sur- face with a thickness of  10 nm. Unfortunately, the smallness of the vesicles and their ... Ca 2+ inside the SR against its concentration gradient. The importance of the SR Ca 2+ -pump was realized in the early 1960s by Ebashi and Lipmann [5,6] and, since then,...

Ngày tải lên: 14/02/2014, 14:20

... machines [6]. The group of T4SS is diverse both with respect to delivered substrates (DNA protein complexes or proteins) and recipients, which can either be a bacterium of the same or other species ... an essential role in the immune escape of this persistent pathogen (Fig. 3A). However, the majority of studies were performed on the interaction of H. pylori with cul...

Ngày tải lên: 14/02/2014, 19:20

... point of intervention within the biosynthetic pathway of ADAM10 is provided by directly interfer- ing with the expression of the gene for ADAM10: the promoter region of the gene for ADAM10 has ... for a therapeutic approach in AD [13–15]. Protein structure and gene organization of ADAM10 The enzyme ADAM10 belongs to the subgroup of metzincins within the zinc pr...

Ngày tải lên: 16/02/2014, 09:20

... phosphorylation of the transcription factor c-JUN was attenuated. Upon expression of wild-type JSP1, a subpopula- tion of cells, with the highest levels of the phosphatase, was induced to float off the dish ... which occurred either in the cells or during sample prepara- tion. The singly charged peptide with monoisotopic mass of 846.48, which corresponds to the pr...

Ngày tải lên: 16/02/2014, 14:20

... monomer-to-trimer transition of HSF1 in the olfactory epithelium warrants further investigations. In accordance with the fundamental role of HSF1 in the heat shock response, the require- ment of HSF1 and HSF4 ... By inducing transcription of the genes encoding heat shock proteins (HSPs) that function as molecular chaperones, the HSFs protect the cell from th...

Ngày tải lên: 18/02/2014, 04:20

... analysis confirmed the identity of the purified protein as HSL (data not shown). The yield of pure protein was 3 mg per litre of insect cell culture. The specific activities of the purified protein against TO, ... These specific activities are lower than those previously reported for nontagged 250 kDa 123 150 kDa 100 kDa 50 kDa 37 kDa 25 kDa Fig. 1. Purity o...

Ngày tải lên: 18/02/2014, 11:20