Tài liệu Báo cáo khoa học: The subtle side to hypoxia inducible factor (HIFa) regulation pdf

... ARTICLE The subtle side to hypoxia inducible factor (HIFa) regulation Rebecca L. Bilton and Grant W. Booker Department of Molecular Biosciences, The University of Adelaide, Australia The transcription ... University of Adelaide, Australia The transcription factor hypoxia inducible factor a-subunit (HIFa) is pivotal in the cellular response to the st...

Ngày tải lên: 20/02/2014, 23:20

... they can increase the amount of available phosphate by interacting together. Additionally, fusing PhyH-DI to a single-domain phytase appears to be an efficient way to improve the activity of the ... We focus on the function and relationship of the two single domains. Additionally, the possibility that fusion of the PhyH N-terminal domain to other single-domain BPPs would...

Ngày tải lên: 14/02/2014, 15:20

... of the tomato SlPti1 in tobacco caused enhanced HR in leaves when challenged with P. syrin- gae pv tabaci expressing AvrPto [17]. On the other hand, expression of the tomato SlPti1 cDNA in the rice ... fused to the GAL4 DNA-binding domain or the empty vector pBD, with PTI1-1 or PTI1-4 fused to the activation domain or the empty vector pAD. (B) Yeast two- hybrid assays with...

Ngày tải lên: 14/02/2014, 19:20

... mutation of the CHR leads to a more pronounced deregulation than the alteration of the CDE [40]. Another gene tested for its cell cycle regulation is the cellular inhibitor of apoptosis protein ... bind- ing to the CHR, cooperating with E2F4 binding to the CDE in EMSAs in vitro [86]. ChIP experiments on the Cdc2 gene also provided insights into the association of other...

Ngày tải lên: 16/02/2014, 09:20

... [16,17]. The BH3 domain-only proteins are a subfamily of the Bcl-2 family involved in the initiation of apoptosis through the mitochondrial pathway. The key event in the mito- chondrial pathway is the ... pathway is the release of proapoptotic fac- tors from the mitochondrial intermembrane space into the cytosol, resulting in the downstream activation of a family of cyto...

Ngày tải lên: 16/02/2014, 09:20

... performed to determine the role of the ARE II and ARE IV sites in the binding of proteins to the ‘extended ARE’. Binding of cytoplas- mic proteins to the ‘extended ARE’ probe was first shown to be ... not shown) to an extent similar to that seen previously with the b-globin and the b-globin ARED transcripts. Moreover, cloning the 108 nucleotide ‘extended ARE’ into...

Ngày tải lên: 16/02/2014, 09:20

... in the UTRs of the transcript and the trans-acting factors (RNA-binding proteins and noncoding regulatory RNAs) that bind to these sequences. Among the best studied regulatory sequences, the ... was comparable to that obtained upon tethering of TTP to the same reporter mRNA. These results indicate that ASF ⁄ SF2 is a potent repressing factor in the absence of competing f...

Ngày tải lên: 16/02/2014, 15:20

... shell; ± 0.05 A ˚ , outer shell), N is the number of scatterers around the central atom, 2d 2 is the the Debye–Waller factor in A ˚ 2 , ± 25%, and the R factor is the least-squares residual, which ... Cd 6 -CeMT-2 and other MTs (Table 2), even when the effect of the over-metallated species is taken into account. To date, the overall stability of cadmium binding to CeMT...

Ngày tải lên: 16/02/2014, 15:20

... procheck [61], was found to be satisfactory. After loop refinement, the final model presented 89.1% of the residues in the most favored regions of the Ramachandran plot, 10.9% in the additional allowed ... The gene was cloned into the expression vec- tor pET-15b to make pATF-20, and the final construct was transformed into E. coli Tuner (DE3). The recombi- nant McoP was pur...

Ngày tải lên: 18/02/2014, 04:20

... desulfurase (OsiS). Results Alr2505 belongs to the NifS-like protein family In a previous study, we began to investigate the contri- bution of the pkn22 operon to the response of Anabae- na to oxidative stress. The pkn transcriptional ... processes: the nifS gene, as explained above, and the alr2495 (sufS) gene (Fig. 4B). In addi- tion to the sufS gene, the former...

Ngày tải lên: 18/02/2014, 04:20