Tài liệu Báo cáo khoa học: Oxygen-dependent regulation of hypoxia-inducible factors by prolyl and asparaginyl hydroxylation pdf

... Mahmood 4 and Jyotdeep Kaur 1 1 Department of Biochemistry, Postgraduate Institute of Medical Education and Research, Chandigarh, India 2 Department of Gastroenterology, Postgraduate Institute of Medical ... of Medical Education and Research, Chandigarh, India 3 Department of Histopathology, Postgraduate Institute of Medical Education and Research, Chandigarh, India 4 De...

Ngày tải lên: 18/02/2014, 16:20

... effects of oxidation and acts as a redox sensor [8,13,32]. Oxidation of RPTPa-D2, like PTP1B, results in the formation of cyclic sulfenamide at the catalytic site, which is stable and reversible by ... blotted. The blots were stained with Coomassie blue and protein bands of interest were cut out and sequenced by Edman degradation at Department of Lipid Chemistry, Utrecht...

Ngày tải lên: 18/02/2014, 17:20

... of HO-2 mRNA and protein and induced the expression of HO-1 mRNA and protein. In contrast, the scrambled HO-2 siRNA did not affect the expression of HO-1 and HO-2 mRNAs and proteins in HeLa and ... role of free heme in expression of HO-1 To explore the role of free heme in HO-1 and HO-2 expression, we treated HeLa and HepG2 cells with SA and determined the expres...

Ngày tải lên: 19/02/2014, 05:20

... sum up some of the available data and mechanisms identified in leukemic stem and leukemic tumor cells and link this informa- tion with the known functions of mixed lineage leukemia and certain ... for example, HoxA7 and HoxA9. The transforming capac- ity of the tested MLL fusion constructs was also dependent on the presence of Meis1 and Pbx proteins, as well as on the prese...

Ngày tải lên: 16/02/2014, 14:20

... process of C-peptide and ana- lyzed the formation of oligomers and their secondary structure by complementary methods, including the detection of C-peptide oligomers by PAGE electropho- resis and ... distribu- tion of 100 l M proinsulin C-peptide in the presence of divalent Ca 2+ and Mg 2+ ions under native conditions (B), and of 100 l M C-peptide in the presence of...

Ngày tải lên: 18/02/2014, 04:20

... those of the quinolone class of antibiotics acting on DNA gyrase, are potentiated by ATP and modulated by chemical agents that affect cell levels of ATP led us to verify the effects of HN and related ... 3E,F). Time dependence of the bactericidal activity of H4-(86–100) and ciprofloxacin In order to evaluate the rapidity of action of his- tone H4 peptides and compar...

Ngày tải lên: 18/02/2014, 14:20

... rabbit preimmune and post-third inoculation sera against a M. tuberculosis sonicate (lanes 1, 2 and 5) and a membrane frac- tion (lanes 3 and 4). Lanes 1 and 3, absence of recognition of M. tuberculosis ... location of Rv2560 and its transmembrane topol- ogy. Although signalp did not predict the existence of a signal peptide, the high grand average of hydropath- icity (G...

Ngày tải lên: 18/02/2014, 16:20

... adversely affect cellular function by direct effects on a variety of enzymes and induction of apoptosis [4]. Tight regulation of intracellular concentrations of free LCACs by acyl-CoA-binding protein ... control of J o increased by 67% and 55% with suc- cinate and glutamate plus malate, respectively, whereas the control of J p was affected more strongly: it increased...

Ngày tải lên: 19/02/2014, 05:20

... used two met- rics: 1) Precision and recall rates of the error correc- tion by the systems, and 2) Relative improvement, the number of differences between improved and de- graded particles in the ... modification candidates are obtained by referring to a phrase table. This table, called the confusion set (Rozovskaya and Roth, 2010a) in the error correction task, stores pairs o...

Ngày tải lên: 19/02/2014, 19:20

... 40 nucleotides upstream and downstream of POS5 was amplified by PCR from plasmid pCgLEU2 by using primers pos5leu21.6f and pos5leu21.6r and was introduced into BY4 742 and MK933 (utr1yef1 YCp-UTR1) ... disruption of each gene was confirmed by PCR [34]. Construction of other plasmids and strains UTR1, POS5 and YEF1 flanking 503 bp, 406 bp and 503 bp upstream of each g...

Ngày tải lên: 20/02/2014, 01:20