Tài liệu Báo cáo khoa học: Enhanced expression of Mcm proteins in cancer cells derived from uterine cervix docx

... level of Mcm expression in HeLa cells and cancer cells from human uterine cervix. Although it remains to be determined how enhanced expression of Mcm proteins affects DNA replication in cancer cells, ... in cancer cells derived from uterine cervix and in HeLa cells. The expression of Mcm genes in growth-arrested cells is induced b...

Ngày tải lên: 20/02/2014, 23:20

... suggesting that the heterologous expression of genes encoding metallo- proteins can lead to incorrect metal ion incorporation. This is observed in rubredoxin and desulforedoxins where zinc, instead ... of protein uti- lizing the principle of protein-dye binding. Anal Bio- chem 72, 248–254. 48 Laemmli UK (1970) Cleavage of structural proteins during the assembly of the head...

Ngày tải lên: 15/02/2014, 01:20

... of the enzyme [7]. Having previously cloned the mph gene from Ochrobactrum sp. M231 [7], we sought to increase the thermostability of this MPH using pro- tein engineering. The two main protein-engineering ... Enhanced thermostability of methyl parathion hydrolase from Ochrobactrum sp. M231 by rational engineering of a glycine to proline mutation Jian Tian, Ping Wang, Shan Gao, X...

Ngày tải lên: 15/02/2014, 01:20

... Molecu- lar cloning and cold-inducible gene expression of ferritin H subunit isoforms in rainbow trout cells. J Biol Chem 271, 26908–26913. 13 Tang SJ, Sun KH, Sun GH, Lin G, Lin WW & Chuang MJ ... pattern of proportional pooling of subunits with different kinetic and thermodynamic properties of the tetrameric enzyme may result in fine-tuning of the properties of skeleta...

Ngày tải lên: 19/02/2014, 02:20

... 161 Determination of phosphatidylserine expression and cell death Phosphatidylserine expression on the outer part of the plasma membrane was examined by Annexin V binding. Briefly, cells were ... study showing the percentage of Perls’ positive HepG2 cells with time of culture in iron-rich media. A total of 200 cells were counted in each time point. (B) For ROS determina...

Ngày tải lên: 19/02/2014, 16:20

... differentiation was initiated at 48 h post-transfection by maintaining the cells in culture medium containing 2% horse serum. Cells were fixed on day 4 of differentiation and stained with hematoxylin and eosin. ... We further investigated the kinetics of miR-27 expression during adipogenesis using quantitative real-time PCR. As shown in Fig. 1C,D, expression of both miR-27a a...

Ngày tải lên: 18/02/2014, 08:20

... analysis of the purified proteins indicated that they contained < 5% contaminating proteins. The concen- trations of proteins used in these studies were determined by spectrophotometric methods using ... overall activity of chaperone proteins. Of the target proteins tested, Arg-HCl was found to specifically increase the activity of aB-crystallin against DTT-induced precip...

Ngày tải lên: 18/02/2014, 16:20

... proteins. B. cereus thus provides a convenient model organism for studying proteins potentially associated with the pathogenicity of the highly infectious B. anthracis. The zinc-binding protein of ... determination of the BcZBP protein at a resolution of 1.8 A ˚ , provides important clues towards understanding the enzyme function, including the identification of a zinc-binding sit...

Ngày tải lên: 19/02/2014, 00:20

... neutralizing action of the cytosolic RNase inhibitor (cRI), a 50-kDa protein containing 16 leu- cine-rich repeat motifs [7,8]. In fact, onconase from Rana pipiens eggs [9], and seminal RNase from Bos ... of EIE were recorded at 30° inter- vals of r from 0 to 360°; (d) the inclination of the dimer major axis with respect to the z axis, hence to the membrane plane, was changed (...

Ngày tải lên: 19/02/2014, 06:20

... levels within 24 h, indicating only transient diminished expression of the PfG6PD-6PGL gene. PfG6PD-6PGL silencing caused arrest of the trophozoite stage and enhanced gametocyte formation. In addition, ... to silencing the PfCLAG9 gene [23], inhibiting PfCLAG9 mRNA translation and diminish- ing cytoadherence of the protein to melanoma cells (a function associated with this prote...

Ngày tải lên: 19/02/2014, 07:20