Tài liệu Báo cáo khoa học: Endotoxic activity and chemical structure of lipopolysaccharides from Chlamydia trachomatis serotypes E and L2 and Chlamydophila psittaci 6BC pdf

... prostaglandin E 2 [10]. These reported data were obtained on LPS of unknown chemical structure and of ill- defined purity. Therefore, in this study we have compared the endotoxic activities of three chlamydial ... L 2 and Chlamydophila psittaci 6BC Holger Heine, Sven Mu¨ ller-Loennies, Lore Brade, Buko Lindner and Helmut Brade Research Center Borstel, Center for Medic...

Ngày tải lên: 20/02/2014, 23:20

... same except that all incubations were performed at 4 °C until the end of the ®xation procedure. For direct detection of ¯uorescein-labeled or rhodamine-labeled peptides, cells were washed three ... was reached when three or four molecules of a 35-amino-acid Tat peptide (sequence 37±72) were chemically coupled to a large protein cargo. Despite the presence of the basic region (sequence...

Ngày tải lên: 21/02/2014, 03:20

... protease. Moreover, the efficiency of generation of 30 K in the intermolecular reaction experiment seems less than that observed in the experiment in which mGFP–calpain 7 was incu- bated, and we ... were detected by CBB staining. Coimmunoprecipitation assay One day after HEK293T cells had been seeded, they were transfected with 5 lg of expression plasmid DNA. After 24 h, cells were har...

Ngày tải lên: 18/02/2014, 04:20

... chelatase reaction requires ATP hydrolysis. The observations are consistent with earlier observations with pea magnesium chelatase where the magnesium chelatase reaction was demonstrated to be ... heterozygous plants carrying the semidominant allele are pale green. It has been shown that the recessive mutations prevent transcription of the Xantha-h gene [24], while the semidominant alleles...

Ngày tải lên: 19/02/2014, 12:20

... an emission wavelength of 440 nm. Background measurements generated from the reaction completed in the absence of substrate were assessed and subtracted from experimental values. Typical values ... synchronize cells, exponentially growing Jurkat 9.2 cells were seeded at a density of 1 · 10 6 per mL. The proliferative status of the cells was determined by assessing the extent of...

Ngày tải lên: 19/02/2014, 13:20

... butyryl- cholinesterase at low pH in the presence of excess substrate remains to be elucidated. Keywords: butyrylcholinesterase; excess substrate activation; mutant enzyme; pH dependence; steady-state kinetics. Human ... (catalog number MET-674B). All other chemicals, including buffer components, were of biochemical grade. Mutagenesis and expression of recombinant BuChE Mutagenesis a...

Ngày tải lên: 20/02/2014, 23:20

... physiologically relevant hypoxia. The heme aa 3 content of SMP directly represents the enzyme-associated heme. In both PC12 cells and macrophages a 38–55% decrease in heme aa 3 content was seen after 10 h of ... nm of 21.1 m M )1 Æcm )1 andthevaluesexpressedasmolÆmin )1 Æmg )1 protein. For the measurement of heme aa3 levels, the SMP were solubilized in laurylmaltoside and heme aa3...

Ngày tải lên: 20/02/2014, 23:20

... Results are means ± SDs of three independent experiments. **P < 0.01. (E) Rat adipocytes were treated with troglitazone for 96 h at the indicated concentrations, and proteins were detected ... into the nucleus, and activates multiple genes, especially adipogenic genes [19]. In the mature adipocyte, PPARc regulates the expression of genes involved in lipid metabolism [19]. Besides i...

Ngày tải lên: 16/02/2014, 09:20

... lobes, one of which is composed of residues from the SET-I region and the other of residues from the SET-C and post-SET regions. Lysine 4 of histone H3 is inserted into a chan- nel, at the end of ... diverse cellular processes rang- ing from stem cell differentiation to metazoan devel- opment and cancer, there has been an intense interest in understanding how SET1 family...

Ngày tải lên: 16/02/2014, 14:20

... normalized to Renilla lucifer- ase activity and is presented as relative luminescence units. Each transfection was performed in duplicate and two inde- pendent measurements were read per sample. All ... The western blot presented is a representative of three independent experiments. Pro-MMP2 activation and zymography HT1080 cells (1 · 10 5 cells) in DMEM and 10% fetal bovine serum...

Ngày tải lên: 18/02/2014, 04:20