... Mrvalues of the standards in thousands. Lanes 2–9, 5 lg of peakP I, P II, P III ⁄ P IV and a mixture of other isoforms (*), respec-tively, in the presence (lanes 2–5) or absence (lanes 6–9) of 2-mercaptoethanol. ... similarity of the A- and B-chains of the four isoforms, a pairwise alignmentwas performed and the values of identity expressed inFig. 3. (Continued).Characterization of four pulchellin isoforms ... III and P IV isoforms from the eluate P III ⁄ P IV (Fig. 1C).Isoelectric focusing gave pI of 5.8, 5.7, 5.5 and 5.2 forthe four isoforms respectively.Secondary structure of the pulchellin isoformsand...