... lg of plasmid DNAusing 10 lg of lipofectamine (Invitrogen, Burlington,Canada), according to protocols supplied by the manufac-turer. In short, the DNA was mixed with lipofectamine andPABP expression ... basis of ligand recognition by PABC, a highly specific peptide-binding domain found in poly(A)-binding protein and aHECT ubiquitin ligase. EMBO J 23, 272–281.9 Craig A (1998) Interaction of polyadenylate-bindingprotein ... AU-richelement-binding polypeptide AUF1 may controlmRNA stability by binding to PABP1 [7]. In light of the vital roles of PABP1 in mRNAmetabolism, it is critical to control the expression of PABP1. Expression...