Tài liệu Báo cáo khoa học: Expression of two [Fe]-hydrogenases in Chlamydomonas reinhardtii under anaerobic conditions doc

... Expression of two [Fe]-hydrogenases in Chlamydomonas reinhardtii under anaerobic conditions Marc Forestier 1, *, Paul King 1 , Liping Zhang 1 , Matthew Posewitz 1 , ... provides information on the expression of the two C. reinhardtii hydrogenases under three different physiological conditions. Materials and methods Cell growth and anaerobic induction Chlam...

Ngày tải lên: 20/02/2014, 11:20

... lg of plasmid DNA using 10 lg of lipofectamine (Invitrogen, Burlington, Canada), according to protocols supplied by the manufac- turer. In short, the DNA was mixed with lipofectamine and PABP expression ... basis of ligand recognition by PABC, a highly specific peptide- binding domain found in poly(A)-binding protein and a HECT ubiquitin ligase. EMBO J 23, 272–281. 9 Craig A (1998)...

Ngày tải lên: 18/02/2014, 12:20

... support the involve- ment of AGN13.2 and not AGN13.1 in the mycopara- sitic process under these conditions. The differential expression of the two AGN13 orthologues in two strains representing two different ... in conditions of carbon and nitrogen source star- vation and chitin induction (Fig. 2). Regulation studies carried out in the solid phase show that agn13.2 i...

Ngày tải lên: 19/02/2014, 16:20

... shown that interactions between KCs and endotoxin comprise the initiating event lead- ing to hepatotoxicity in liver injury, including endotox- emia and ischemia ⁄ reperfusion injury [14]. In our studies, ... mediator of lethal sepsis, triggering the production of proinflammatory cytokines and liver injury [13]. Therefore, the present study aimed to elucidate the role of KCs in the...

Ngày tải lên: 14/02/2014, 18:20

... long-chain saturated fatty acids (SFAs) led to increased expression of proin- flammatory cytokines, inhibition of insulin signaling, induction of endoplasmic reticulum (ER) stress, and promotion of ... elevations in the expression of activating transcription factor 4 (ATF4) and of CCAAT⁄ enhancer-binding protein homologous protein (CHOP) proteins downstream of eIF2a were ob...

Ngày tải lên: 14/02/2014, 22:20

... role in skin wound healing. After a skin injury, PPARb expression is rap- idly elevated in the epidermis at the wound edges; dele- tion of a single PPARb allele results in delayed wound healing ... of CERK expression by activa- tion of PPARb results in a decrease in intracellular Cer levels and enhancement of cell survival. Results Skin barrier disruption in hairless m...

Ngày tải lên: 18/02/2014, 18:20

... coat protein (g3p) are responsible for binding and infection in E. coli.Thus, incorporation of a library of target proteins between the N-terminal domain and the C-terminal (CT) domain allows ... Protein design by binary patterning of polar and non- polar amino acids. Science 262, 1680–1685. 8. Michnick, S.W. (2001) Exploring protein interactions by inter- action-induced folding of...

Ngày tải lên: 19/02/2014, 12:20

... Zn 2+ in the p resence of ferrocyanide. We have ob tained evidence that isoalloxazine and t he surrounding amino acids are the binding site of the inhibitor. Clearly, the observation that binding of ... comprise two structural domains, each containing % 150 amino acids [11]. The C-terminal region includes most of the residues i nvolved in NADP(H) b inding, and the large cleft be...

Ngày tải lên: 19/02/2014, 16:20

... made up of two f unctional domains, one corresponding to the cofactor-binding domain, or Rossman fold (residues 1–147 and 313–334 in spinach GAPDH (accession code in PDB:1JN0), the other being the catalytic ... mechanism of GAPDH could be essential for the binding of CP12. The presence of this region at the interface of GAPDH and CP12 could also explain the kinetic changes o b...

Ngày tải lên: 19/02/2014, 16:20

... everything the user needs to perform an- notation tasks is included within the software. 2 The Project Window In the majority of cases, the annotator is interested in annotating a range of texts, ... on the main window opens a Corpus Search interface, which allows users to retrieve lists of segments matching a query. Queries can involve multiple layers, for instance, subject in...

Ngày tải lên: 20/02/2014, 09:20