Tài liệu Báo cáo khoa học: Mechanism of protection of peroxidase activity by oscillatory dynamics docx

... defined as the amount of soluble protease required to release 1 lg of soluble azo- peptidesÆmin )1 . The specific activity was the number of units of activity per milligram of protein. For specific ... optimum pH and temperature of activity of each prote- ase. In brief, digestion by Erv-A, Erv-B, Erv-C were carried out at pH 8.0 and 37 °C for 2 h and blocked by E-64; dige...

Ngày tải lên: 14/02/2014, 14:20

... deuteration of C2¢ of the epimerase product. The lack of deuteration of the C2¢ of DHMP is not due to the lack of deute- rons, because it has been shown previously that the 6-hydroxymethyl group of the ... product is generated by the re-formation of the C–C bond after the reorientation of GA, which exposes the opposite face of the aldehyde. Y. Wang et al. Mechanism and...

Ngày tải lên: 19/02/2014, 00:20

... Mechanism of protection of peroxidase activity by oscillatory dynamics Lars F. Olsen 1,2 , Marcus J. B. Hauser 3 and Ursula Kummer 1 1 European ... observation of long time intervals of oscillatory dynamics. Numerical simulations In order to understand the mechanism of protection better andtobeabletodepicttheroleofthearomaticcofactorin this ... concentra- tion...

Ngày tải lên: 20/02/2014, 11:20

... Composition of nucleosomes. The assembly of the histone octamer on DNA is represented by this model which depicts the incorporation of two H3 ⁄ H4 dimers with an inner core of  60 bp of DNA, followed by ... recruitment of MOF to recently transcribed regions, especially at the 3¢ ends of genes where H3-K36me3 is enriched [128]. In mammalian cells MOF also exists as part of...

Ngày tải lên: 14/02/2014, 18:20

... summary, the proposed mechanism of Cu A assembly consists of the sequential insertion of two Cu(I) ions donated by a metallochaperone into the Cu A centre, whose cysteines are kept reduced by a Sco protein ... is not affected by the loss of the histidine ligand, it was argued that the Cu(II)-bound state has a major role in the mechanism of action of BsSco, ruling out the...

Ngày tải lên: 14/02/2014, 18:20

... expression of estrogen receptor-a (ERa) is autoregulated by miR-18a, -19b and -20b, which in turn are upregulated by the activation of ERa. This mechanism of regulation provides a wide range of coordinated ... the fine-tuning of p53 activity [45,46]. miR-34a is also induced by a p53-independent pathway: ELK1, which is a member of the ETS family of transcription factors,...

Ngày tải lên: 14/02/2014, 19:20

... were selected. MALDI-TOF/TOF In addition to LC-MS ⁄ MS analysis, we also completed TOF ⁄ TOF analysis of selected peaks to confirm structure using a MDS Sciex 4800 MALDI-TOF ⁄ TOFÔ instru- ment (Applied ... FEBS 1699 of RBCs occurs when the addition of a limiting amount of virus results in ‘crosslinking’ of RBCs through binding of multiple RBCs to HAs present on a single virus; mea...

Ngày tải lên: 14/02/2014, 19:20

... 30% of the X 6 was hydrolyzed. After 60 min of incubation, the reaction was terminated. For XBS samples, this was carried out by the addition of 20 lL of NaOH (2.00 m) and subsequent boiling of ... FEBS Activity on Xylazyme AX After 10 min of pre-incubation, a Xylazyme AX tablet was added to 1.0 mL of an appropriate enzyme dilution. The reaction was terminated by the addit...

Ngày tải lên: 14/02/2014, 19:20

... the promoters of cyclin B1 and cyclin B2 in human tissues according to ChIP, followed by identification of genes by genome-wide DNA-microarray hybridization [87]. However, the limitation of ChIP analyses ... regulator of CDE ⁄ CHR cell cycle genes, to which promoter sites does it bind during any par- ticular phase of the cell cycle? What is the exact mechanism of p53-dependen...

Ngày tải lên: 16/02/2014, 09:20

... indicated by a burst of mouse 3T6 fibroblasts transfected with wt or mutated MPyV genomes, was followed by LDH release. Values of LDH release are presented relative to those obtained by treatment of ... levels of apoptotic markers during the late stages of MPyV infection. However, comparison of the levels of apoptotic markers and cytotoxicity during the replication of wt...

Ngày tải lên: 16/02/2014, 09:20