Tài liệu Báo cáo khoa học: Mutational analysis of plasminogen activator inhibitor-1 Interactions of a-helix F and its neighbouring structural elements regulates the activity and the rate of latency transition pdf

... Mutational analysis of plasminogen activator inhibitor-1 Interactions of a-helix F and its neighbouring structural elements regulates the activity and the rate of latency transition Troels ... amino acid sequence of a-helix F (hF) and the connecting loop to s3A (hF/s3A-loop) for the rate of latency transition. We grafted regio...

Ngày tải lên: 20/02/2014, 11:20

... this work for the activation of ONC has no detrimental effect on the conformational stability of the enzyme. Cytotoxic activity The effect of (Met1) removal on the cytotoxic properties of ONC was ... of the hydrolysis was confirmed by the disappearance of the (Met1)-ONC (M23L) signal in the MALDI-TOF mass spectra 1 h after the addition of AAP. The pH was a...

Ngày tải lên: 19/02/2014, 12:20

... Lopiano 4 and Mauro Fasano 1 1 Department of Structural and Functional Biology, and Centre of Neuroscience, University of Insubria, Busto Arsizio, Italy 2 Department of Biotechnology, University of Verona, ... impairment of the antioxidant defense of the cell, as in the case of DJ-1 or PTEN-induced putative kinase 1 mutations. Mitochondrial and proteasome dysfu...

Ngày tải lên: 15/02/2014, 01:20

... regardless of the type of substrate (acetyl amide or acetyl ester). Results and Discussion Action of reversible effectors on AAA activity of BuChE The investigation of the effects of the ligands (tyramine, ... could reflect differences in the conformational plasticity of the active site gorge of these enzymes. Effects of benzalkonium on o-NPA and o-NAC hydro...

Ngày tải lên: 18/02/2014, 17:20

... demonstrating that the J–I rise is not directly related to the reduction of the PQ pool. A further analysis of the influence of PQ reduction on FI was performed after the addition of dPQ to the thylakoid ... raised. The effect of DCMU on the traces was to increase the amplitude of step J with the concurrent decline of step I, and to retard the rise to F...

Ngày tải lên: 19/02/2014, 05:20

... concentration of the porphyrin was 0.62 m M ), to investigate the effect of porphyrin b inding on the protein conformation. For these spectra, a spectrum of the buffer containing the same concentration of ... structures of MCL. To obtain more quantitative information on the secon- dary structure of MCL and the effect of ligand binding on it, the far-UV CD spect...

Ngày tải lên: 19/02/2014, 16:20

... ÔStains- AllÕ analysis of Fig. 3 and the 1D i mmunoblotting of Fig. 4. As the full-length Dp427 isoform of dystrophin does not enter the second dimension of conventional 2D gels, the expression level of ... viscosity of t he extract as a result of D NA, 2 lLof DNase I (200 units) was added per 100 lL of buffer [30]. Following filtration through two layers of mirac...

Ngày tải lên: 19/02/2014, 16:20

... with BMAL1 and the bin ding to the E-box. Substitution of Arg65 for Ala abolished the interaction of DEC1 with BMAL1 and the binding of DEC1 to the E-box. However, substitution of His57 for Ala ... expression vector and 50 ng of FLAG-BMAL1 expression vector resulted in adequate levels of expression. The transcriptional activity of FLAG-BMAL1, and the supp...

Ngày tải lên: 19/02/2014, 16:20

... combination of a hierarchical up-regulation [16] of the las operon at low PK activity and a polar effect of the pyk deletion on expression of ldh.An important question is whether this affects the conclu- sions ... higher than the flux control coefficients for the individual las enzymes at comparable levels. From the data for PFK given in Andersen et al. [9], the flux co...

Ngày tải lên: 19/02/2014, 17:20

... extension of this comparison to other parts of speech and to punctuation, we can pre- dict the correct equivalent of и in 90% of its occurrences. Other examples of structural clarification of this ... governs the genitive case. The task of deciding whether or not to retain the &apos ;of& apos; falls upon the reader. The problem results, of course, fro...

Ngày tải lên: 19/02/2014, 19:20