Tài liệu Báo cáo khoa học: Calcium signalling by nicotinic acid adenine dinucleotide phosphate (NAADP) doc

... elicited by the NADP metabolite nicotinic acid adenine dinucleo-tide phosphate. Proc Natl Acad Sci USA 97, 8693–8698.17 Johnson JD & Misler S (2002) Nicotinic acid- adenine dinucleotide phosphate- sensitive ... MINIREVIEW Calcium signalling by nicotinic acid adenine dinucleotide phosphate (NAADP) Michiko Yamasaki, Grant C. Churchill and Antony GalioneDepartment ... messenger, nicotinic acid adenine di-nucleotide phosphate. J Biol Chem 277, 43717–43723.31 Patel S, Churchill GC & Galione A (2000) Uniquekinetics of nicotinic acid adenine dinucleotide...

Tài liệu Báo cáo khoa học: Calcium-binding to lens bB2- and bA3-crystallins suggests that all b-crystallins are calcium-binding proteins pptx

... should not bind calcium. In order to probewhether b-crystallins can bind calcium, we selected one basic (bB2) andone acidic (bA3) b-crystallin for calcium- binding studies. Using calcium- binding ... bind calcium with varying afﬁnity,thus suggesting that all b-crystallins would bind calcium. Calcium- binding does not inﬂuence proteinconformation, a property exhibited by some of the calcium- binding ... using isothermal titrationcalorimetry.Probing calcium- binding by Stains-All assay Calcium- binding to bB2- and bA3-crystallins was eval-uated by calcium probe Stains-All, a carbocyanine dye[29]....

Tài liệu Báo cáo khoa học: Calcium-independent phospholipase A2 mediates proliferation of human promonocytic U937 cells pptx

... paracrine stimulation ofarachidonic acid mobilization by secreted phospholi-pase A2is regulated by cytosolic phospholipase A2-derived hydroperoxyeicosatetraenoic acid. J Immunol171, 989–994.55 ... werequenched by adding 3.75 volumes of chloroform ⁄ methanol(1 : 2). After lipid extraction, free [3H]palmitic acid wasseparated by TLC using n-hexane ⁄ ethyl ether ⁄ acetic acid (70 : 30 ... PLA2activities [30,49–51]. After lipidextraction, free [3H]arachidonic acid was separated by TLCusing n-hexane ⁄ ethyl ether ⁄ acetic acid (70 : 30 : 1) as themobile phase [52,53]. For Ca2+-independent...

Tài liệu Báo cáo khoa học: Calcium-independent cytoskeleton disassembly induced by BAPTA pdf

... gene expression and synaptic t ransmission d ependon calcium concentrations [1]. Calcium chelators are widely used to probe the role of calcium signalling in cell functions [ 2,3]. Such chelatorsprincipally ... propose thatD-BAPTA s hould b e systematically introduced in calcium signalling experiments, as controls for the known andunknown calcium independent eﬀects of BAPTA. Addi-tionally, the concomitant ... Ca2+ signalling is central to cell physi-ology. The Ca2+chelator 1,2-bis(2-aminophenoxy)ethane-N,N,N¢,N¢-tetraacetic acid (BAPTA) has been widely used asa probe to test the role of calcium...

Tài liệu Báo cáo khoa học: Mechanisms of amyloid ﬁbril formation – focus on domain-swapping doc

... supported by programs P1-0140 (prote-olysis and its regulation, led by B. Turk) and P1-0048(Structural Biology, led by D. Turk) and by the projectJ3-2258 (V. Stoka), all ﬁnanced by the SlovenianResearch ... Proteins and lipids becomedamaged by oxidative stress and by excessive metalinteractions, which, in turn, could both promote pro-tein aggregation [7,8]. Aging by itself inﬂuences theperformance ... ﬁbrils has been deter-mined in a limited number of cases by either solid stateNMR [25] or by H ⁄ D exchange quenched ﬂow fol-lowed by heteronuclear NMR [26]. However, the struc-ture of the...

Tài liệu Báo cáo khoa học: Neuronal growth-inhibitory factor (metallothionein-3): structure–function relationships docx

... abolished by replacing Thr5 with Ala5[15,18]. This contradiction raises the question of whythe bioactivity of sGIF is unaffected by replacement ofThr5 with Ala5. After comparison of the amino acid sequence ... unique acid basic catalysis motif in the b-domain:KCE(21–23) [16]. To understand the role of the acid basic catalysis motif in S-nitrosylation, we constructedan E23K mutant protein of hGIF by ... biological function of the unique TCPCP motif. By contrast, the biochemical properties and biologicalfunctions of the EAAEAE insert of GIF are poorlydocumented. NMR data showed that the backboneconformation...

Tài liệu Báo cáo khoa học : Cần gì làm khi chọn làm quan thời nay doc

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Tài liệu Báo cáo khoa học: The Saccharomyces cerevisiae vacuolar acid trehalase is targeted at the cell surface for its physiological function docx

... hydrolyzed to glucose by the action of two types of trehalase: ‘neutral treha-lases’ encoded by NTH1 and NTH2 [3,7], which areoptimally active at pH 7, and acid trehalases’ encoded by ATH1, which ... suc2-D9 Gift of H. Bussey BY4 741 ath1D MAT a his3-D1 leu2-D0 ura3-D0 met15-D0 ath1D::KanMX4 This study BY4 741 vps4D MAT a his3-D1 leu2-D0 ura3-D0 met15-D vps4D::KanMX4 Euroscarf BY4 741 ATH1_GFP MAT ... the wild-type strain BY4 741 or vps4D mutant by homolo-gous recombination, for C-terminal tagging of Ath1 withGFP or mCherry. The path1DN–mCherry vector wasconstructed by in vivo homologous...

Tài liệu Báo cáo khoa học: Prion protein library of recombinant constructs for structural biology docx

... have been inspired by natural varia-tions in mammalian PrP amino acid sequences. HumanPrP fragments of variable lengths have been used tostudy the minimal length of the amino acid sequencethat ... puriﬁed by cation exchange chromatography in thepresence of urea, and oxidized at low concentrations by airoxygen in the presence of 1 lm CuSO4. The oxidized proteinwas puriﬁed by cation ... and refolded by afﬁnitychromatography using Ni2+–nitrilotriacetic acid agaroseresin. The N-terminal histidine tail was then cleaved usingthrombin, and the thrombin was removed by ion exchangechromatography....

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