Tài liệu Báo cáo khoa học: Calcium signalling by nicotinic acid adenine dinucleotide phosphate (NAADP) doc

... elicited by the NADP metabolite nicotinic acid adenine dinucleo- tide phosphate. Proc Natl Acad Sci USA 97, 8693–8698. 17 Johnson JD & Misler S (2002) Nicotinic acid- adenine dinucleotide phosphate- sensitive ... MINIREVIEW Calcium signalling by nicotinic acid adenine dinucleotide phosphate (NAADP) Michiko Yamasaki, Grant C. Churchill and Antony Galion...

Ngày tải lên: 20/02/2014, 01:20

... should not bind calcium. In order to probe whether b-crystallins can bind calcium, we selected one basic (bB2) and one acidic (bA3) b-crystallin for calcium- binding studies. Using calcium- binding ... bind calcium with varying affinity, thus suggesting that all b-crystallins would bind calcium. Calcium- binding does not influence protein conformation, a property exhibited by some of...

Ngày tải lên: 18/02/2014, 16:20

... paracrine stimulation of arachidonic acid mobilization by secreted phospholi- pase A 2 is regulated by cytosolic phospholipase A 2 - derived hydroperoxyeicosatetraenoic acid. J Immunol 171, 989–994. 55 ... were quenched by adding 3.75 volumes of chloroform ⁄ methanol (1 : 2). After lipid extraction, free [ 3 H]palmitic acid was separated by TLC using n-hexane ⁄ ethyl ether ⁄ acet...

Ngày tải lên: 18/02/2014, 17:20

... gene expression and synaptic t ransmission d epend on calcium concentrations [1]. Calcium chelators are widely used to probe the role of calcium signalling in cell functions [ 2,3]. Such chelators principally ... propose that D-BAPTA s hould b e systematically introduced in calcium signalling experiments, as controls for the known and unknown calcium independent effects of BAPTA....

Ngày tải lên: 19/02/2014, 16:20

... supported by programs P1-0140 (prote- olysis and its regulation, led by B. Turk) and P1-0048 (Structural Biology, led by D. Turk) and by the project J3-2258 (V. Stoka), all financed by the Slovenian Research ... Proteins and lipids become damaged by oxidative stress and by excessive metal interactions, which, in turn, could both promote pro- tein aggregation [7,8]. Aging by its...

Ngày tải lên: 14/02/2014, 18:20

... abolished by replacing Thr5 with Ala5 [15,18]. This contradiction raises the question of why the bioactivity of sGIF is unaffected by replacement of Thr5 with Ala5. After comparison of the amino acid sequence ... unique acid basic catalysis motif in the b-domain: KCE(21–23) [16]. To understand the role of the acid basic catalysis motif in S-nitrosylation, we constructed an E23K mutan...

Ngày tải lên: 16/02/2014, 15:20

Ngày tải lên: 17/02/2014, 05:20

... hydrolyzed to glucose by the action of two types of trehalase: ‘neutral treha- lases’ encoded by NTH1 and NTH2 [3,7], which are optimally active at pH 7, and acid trehalases’ encoded by ATH1, which ... suc2-D9 Gift of H. Bussey BY4 741 ath1D MAT a his3-D1 leu2-D0 ura3-D0 met15-D0 ath1D::KanMX4 This study BY4 741 vps4D MAT a his3-D1 leu2-D0 ura3-D0 met15-D vps4D::KanMX4 Euroscarf BY4...

Ngày tải lên: 18/02/2014, 06:20

... have been inspired by natural varia- tions in mammalian PrP amino acid sequences. Human PrP fragments of variable lengths have been used to study the minimal length of the amino acid sequence that ... purified by cation exchange chromatography in the presence of urea, and oxidized at low concentrations by air oxygen in the presence of 1 lm CuSO 4 . The oxidized protein was purified b...

Ngày tải lên: 18/02/2014, 08:20