Tài liệu Báo cáo khoa học: Template requirements and binding of hepatitis C virus NS5B polymerase during in vitro RNA synthesis from the 3¢-end of virus minus-strand RNA docx

... hp2bs CCCCTGATGGGGGCGTATTTCCACCATGAATCACTCCCC hp2b hp2br GTGATTCATGGTGGAAATACGCCCCCATCAGGGGGCTGG (–)IRES LDH2s TTTTGCGGCCGCGCCAGCCCCCTGATGGGGGCGCAGCCTCCAGGA LDH2 CCCCCCCTCCCGGGAGAGCCATAGTGGTCTGCGGAACCGGTTTT LDH2r ... 5’341T7 TAATACGACTCACTATAGGGTGCACGGTCTACGAGACCT (–)IRES DSLA1 GCCAGACACTCCACCATGAATCACTCCCCTGTGAGGAACTACTGTCTTCACG DSLA1 5’341T7 TAATACGACTCACTATAGGGTGCACGGTCTACGAGACCT (–)IRES D...

Ngày tải lên: 20/02/2014, 01:20

... [ 125 I]TC-PCSK9 binding increased as cell surface LDLR density increased. Unlabeled wild-type and two gain -of- function mutants of PCSK9 reduced binding of [ 125 I]TC-PCSK9. The Scatchard plot of the binding- inhibition ... HepG2 cells increased following growth in the absence of lipo- proteins, and there was a corresponding increase in the binding of [ 125...

Ngày tải lên: 14/02/2014, 14:20

... other bacteria. Its interaction with macrophages induces a decrease in the production of the pro -in ammatory cytokines tumour necrosis factor-a and interferon (IFN) -c and an over- production of ... to be the only one that is common to all bacteria. In Yer- sinia, the increased synthesis of LcrV triggered by the activation of the system leads to the titration...

Ngày tải lên: 14/02/2014, 22:20

... GTCCAGAATATTCAGCCTTTCACC 3¢-RACE Zftbet-F1 CTCCCTCAAACAAACCAGAGTC Initial PCR Zftbet-R1 CACTGGATGAGACAGGAAGTT Initial PCR Zf3¢tbet-F1 CTTCTCCAGGACAGTCCAAAGAGTC 3¢-RACE Zf3¢ tbet-F2 CTGGATTGAAGCGCCCTCGGTTAATC ... ATGCTCATCAGTAGATTCTGCTCAC Real-time PCR ZFil10-F ACGCTTCTTCTTTGCGACTG Real-time PCR ZFil10-R CACCATATCCCGCTTGAGTT Real-time PCR Zfgata3-F GCTTCTTCCTCCTCGCTGTC Real-time PCR Zfgata3-R...

Ngày tải lên: 16/02/2014, 09:20

... (Roche Diagnostics) and the following PCR primer sets: 5¢-GGAGATCTCCAGTGA TATCGACCA-3¢ and 5¢-ACGGCTTCTACGGATCGAA ACT-3¢ for PPARc ,5¢-AAGACAGCTCCTCCTCGAAGG TT-3¢ and 5¢-TGACCAAATCCCCATTTACGC-3¢ ... aP2, 5¢-ATCCATGGATGGACGGTAACG-3¢ and 5¢-CTGGA TCCCAATACTTCGACCA-3¢ for LPL, 5¢-TGGGTTGG CTGCTTGTG-3¢ and 5¢-GCGTGGGCAGGATGAAG-3¢ for SCD, 5¢-GATGTGGAACCCATAACTGGATTCAC-3¢ and 5¢-GGTC...

Ngày tải lên: 16/02/2014, 09:20

... most convincing evidence supporting this hypothesis was the rescue of the bone mass phenotype of the ob ⁄ ob mice by intracerebroventricular (ICV) infusion of leptin in the hypothalamic region, clearly ... demonstrating that the inhibitory action of leptin on bone formation is medi- ated by a central circuit [25]. Further supporting the importance of leptin in the...

Ngày tải lên: 18/02/2014, 04:20

... cob(II)alamin. Reduction of the Co centre promotes the dissociation of the lower dimethylbenz- imidazole base of the cofactor. Consistent with this, the crystal structure of the cobalamin -binding domain Homocysteine Methionine Co Methylcob(III)alamin Cob(I)alamin Primary turnover cycle H CH 3 H 4 folate AdoHyc Cob(I)alamin Cob(II)alamin H 4 folate Co NADPH A doMet e – FA...

Ngày tải lên: 18/02/2014, 08:20

... levels of both b-conglycinin and glycinin, as pro-b-conglycinin and proglycinin are transient pro- tein forms that are present in the ER prior to process- ing in the protein storage vacuoles. The synthesis ... third amino acids in classic redox- active CGHC motifs with methionine or isoleucine and asparagine in GmPDIL-3a and GmPDIL-3b may be the cause of the la...

Ngày tải lên: 18/02/2014, 11:20

... by SREBP- 1c in the liver inhibits processes regulating insulin signaling, such as glycogen synthesis, and con- tributes to the physiological switching from glycogen synthesis to fatty acid synthesis during ... hepatosteatosis and pancreatic b-cell dysfunction [25,26]. Insulin resistance in liver and impaired insulin secretion in b-cells Molecular dissection of th...

Ngày tải lên: 18/02/2014, 13:20

... employed these toxins (mainly OA) to examine cell–matrix interactions and cell–cell contacts, either directly by controlling struc- tural adhesion proteins, or indirectly by affecting pro- teins involved ... activity. Among the structures implicated in cell–cell interactions, tight junctions are specialized contact sites between the cell membranes of adjacent cells in which the...

Ngày tải lên: 18/02/2014, 14:20