Tài liệu Báo cáo khoa học: Identification of critical active-site residues in angiotensin-converting enzyme-2 (ACE2) by site-directed mutagenesis docx

... Identification of critical active-site residues in angiotensin-converting enzyme-2 (ACE2) by site-directed mutagenesis Jodie L. Guy, Richard M. Jackson, ... CL1 by 20.7 A ˚ in tACE and is coordinated by residues in subdomain I), it may be important for stabilizing complex formation for those residues in subdomain II involved directly in substrate bindi...

Ngày tải lên: 20/02/2014, 01:20

... the guanidini- um group of the Arg in the RGG domain in the C-terminal of EWS. Results and Discussion Several DNA-binding proteins that bind to G-quadru- plex DNA have been investigated in vitro ... the RGG domain of nucleolin, a DNA-binding protein contributing to the transcrip- tion of ribosomal RNA, bind to the G-quadruplex forming ribosomal DNA [26]. Moreover, nucleolin bind...

Ngày tải lên: 15/02/2014, 01:20

... two mutants in bacterial clearance might reflect differences in the integrity of their outer membrane, binding activity and sensitivity of the murine comple- ment-mediated killing. In conclusion, ... After 24 h of incubation, the resulting Zeo r colonies were selected for PCR identification using primers b1X and b1B, and the inactivated lpxX mutant was verified by sequencing. Clo...

Ngày tải lên: 18/02/2014, 14:20

... Raychaudhuri S, Sinha M, Mukhopadhyay D & Bhat- tacharyya NP (2007) HYPK, a Huntingtin interacting protein, reduces aggregates and apoptosis induced by N-terminal Huntingtin with 40 glutamines in Neuro2A cells ... number of genes encoding structural components of the cytoskeleton and proteins involved in contractile functions (including actin, tubulin myosin and profilin) were di...

Ngày tải lên: 18/02/2014, 16:20

... different domains of the protein, to determine the calcium-binding domain of the protein. (A) Schematic drawing of the recombinant proteins. Six GST-fused recombinant proteins containing the three distinctive domains ... P i , and specific binding of the antibody was detected by incubating the sections in a solution of 10 mm diaminobenzidine in 10 mm NaCl ⁄ P i . H. Tohse et al...

Ngày tải lên: 18/02/2014, 17:20

... out by heterologous expression in yeast. Results and Discussion P450 genes induced by elicitors in the cell culture of G. max Accumulation isoflavones phytoalexin glyceollins, in the seedling infected ... understanding of the biosynthesis of triterpene saponins is required, including the enzymes catalyzing the sequence of reactions and the genes encoding these enzymes. The bi...

Ngày tải lên: 19/02/2014, 07:20

... in the absence of the STAT2 DNA binding domain as revealed by real-time PCR To more quantitatively examine the expression of IFN- regulated genes in the absence of the STAT2 DNA binding domain, ... DNA binding, and compared the cDNAs of IFN-treated cells over- expressing intact STAT2 to those of IFN-treated cells overexpressing mutated STAT2 lacking the DNA binding domain. The...

Ngày tải lên: 19/02/2014, 07:20

... FEBS Analysis of serine proteinases secreted by various cell lines Trypsin-type serine proteinases present in the condi- tioned media of the nine cell lines were analyzed by gelatin zymography ... 2006 FEBS 627 Identification of membrane-bound serine proteinase matriptase as processing enzyme of insulin-like growth factor binding protein-related protein-1 (IGFBP-rP1/angiomoduli...

Ngày tải lên: 19/02/2014, 07:20

... 4.9 a This strain was grown in media lacking leucine. The other strains were grown in media containing leucine. Fig. 5. Complementation of pos5 cell. Indi- cated pos5 and WT cells carrying each gene on ... biosynthesis (nicotinic acid mononucleotide, nicotinic acid adenine dinucleotide, nicotinic acid and quinolinic acid) did not inhibit Yef1p (Table 2). HgCl 2 inhibited enzyme activity...

Ngày tải lên: 20/02/2014, 01:20

... the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem. 72, 248–254. 20. Laemmli, U.K. (1970) Cleavage of structural proteins during the assembly of ... in the amino acid sequence, increasing the number of basic residues of lysine, arginine and histidine, could occur. However it seemed probable that the eEF1A isoform...

Ngày tải lên: 21/02/2014, 00:20