Tài liệu Báo cáo khoa học: Identification of ATP-NADH kinase isozymes and their contribution to supply of NADP(H) in Saccharomyces cerevisiae docx

... RBD and the RGG domain of nucleolin, a DNA-binding protein contributing to the transcrip- tion of ribosomal RNA, bind to the G-quadruplex forming ribosomal DNA [26]. Moreover, nucleolin binds to ... the guanidini- um group of the Arg in the RGG domain in the C-terminal of EWS. Results and Discussion Several DNA-binding proteins that bind to G-quadru- plex DNA have been...

Ngày tải lên: 15/02/2014, 01:20

... all-alpha domain, STAT protein DNA-binding domain and SH2 domain of stat6, and the zinc-finger domain, leucine-zipper domain and fork-head domain of foxp3. In addition, for stat6 and foxp3, there ... FOX proteins, a leucine zipper domain and a C2H2 zinc finger domain, both of which are thought to help mediate DNA binding and may be involved in the induction of dimeriza...

Ngày tải lên: 16/02/2014, 09:20

... from chromosomal DNA of O35E using primers b2E and b2B (Table 2, Fig. 1B), and cloned into pCR2.1 using a TOPO TA cloning kit to obtain pCRlpxL. The insertion was released by EcoRI–BamHI digestion, and then ... PCR identification using primers b1X and b1B, and the inactivated lpxX mutant was verified by sequencing. Cloning of lpxL and construction of the knockout mutant O3...

Ngày tải lên: 18/02/2014, 14:20

... representing the 5¢- and 3¢-UTRs are shown in white in the genomic gene and the cDNA. Intron size in bp, domain size in bp and domain size in amino acids are indicated at the bottom of each schematic ... group of structurally related, secreted cytokines, including TGFb, bone morphogenetic protein (BMP), and acti- vin [8–11]. The TGFb signaling cascade is stimulated throu...

Ngày tải lên: 18/02/2014, 16:20

... CA protein sequences. Iden- tical and similar amino acids shared by at least 50% of the isoforms are shown in black and grey, respectively. Histidine residues involved in zinc binding in the catalytic ... reference to posi- tions 94, 96 and 119 in CAII from Homo sapiens) which are directly involved in binding the zinc cofac- tor, are conserved in the two R. pachyptila se...

Ngày tải lên: 18/02/2014, 16:20

... number of genes encoding structural components of the cytoskeleton and proteins involved in contractile functions (including actin, tubulin myosin and profilin) were differentially expressed in C. ... Bhat- tacharyya NP (2007) HYPK, a Huntingtin interacting protein, reduces aggregates and apoptosis induced by N-terminal Huntingtin with 40 glutamines in Neuro2A cells and exhib...

Ngày tải lên: 18/02/2014, 16:20

... determine the calcium-binding domain of the protein. (A) Schematic drawing of the recombinant proteins. Six GST-fused recombinant proteins containing the three distinctive domains of tandem repeat 1 ... to the beads. By contrast, incubation with otolith extract resulted in binding of a complex of the HMW aggregate containing OMM-64 and otolin-1 to the beads. H. Tohse et al....

Ngày tải lên: 18/02/2014, 17:20

... cultures of G. max. (data not shown). The genes obtained were ligated into expres- sion vector pYES2 (Invitrogen) and expressed in S. cerevisiae strain INVSC2 (Invitrogen). However, in vitro assay of ... biosynthesis of triterpene saponins is required, including the enzymes catalyzing the sequence of reactions and the genes encoding these enzymes. The biosynthesis of trite...

Ngày tải lên: 19/02/2014, 07:20

... (ISGs). Upon IFN binding to IFNAR, the receptor-associated kinases tyrosine kinase 2 (Tyk2) and Jak1 phos- phorylate key tyrosine residues within the intra- cellular domains of the receptor subunits ... Characterization of the recombinant extracellular domains of human interleukin-20 receptors and their complexes with inter- leukin-19 and interleukin-20. Biochemistry 42, 126...

Ngày tải lên: 19/02/2014, 07:20

... follows: aprotinin, leu- peptin, pepstatin A, AEBSF and proteinase inhibitor mixture that contains AEBSF, aprotinin, bestatin, E-64, leupeptin and pepstatin A from Wako Pure Chemical Industries (Osaka, ... commercially available proteinase inhibitor mixture containing AEBSF, aprotinin and some other inhibitors inhibited the processing to the same extent as AEBSF alone. As trypsin-type...

Ngày tải lên: 19/02/2014, 07:20