Tài liệu Báo cáo khoa học: Extended half-life upon binding of destabilized intrabodies allows specific detection of antigen in mammalian cells pdf

... Specific antigen detection with destabilized intrabody Extended half-life upon binding of destabilized intrabodies allows specific detection of antigen in mammalian cells Annie-Paule Sibler 1 ,Je ´ ro ˆ me ... the 7A-cotransfected cells, thus demonstrating that strong in vivo binding of the destabilized 13R4-d1-EGFP pro- teins allows them to be m...

Ngày tải lên: 19/02/2014, 18:20

... and of the kanamycin insertion mutant nirF strain (SBN03) in the upper right panel. Growth of SBN11 (DnirF) is shown in the bottom left panel and of SBN13 (DnirF) containing pEG276-nirF in the ... d 1 heme. Replacement of an equivalent His, His41, in NirF by Ala abolished binding of the heme to the protein. Known distal heme -binding residues, such as Met, Lys or Cys [18,...

Ngày tải lên: 15/02/2014, 01:20

... were of two types, either with the two-domain architecture of the RibG protein of Bacil- lus subtilis or the RibD protein of E. coli, respectively [18–20], or the single-domain type of 2,5-diamino-6- ribitylamino-4(3H)-pyrimidinone ... reductase domain is located at the N-terminus and the predicted deaminase at the C-terminus of the bifunctional protein. In plants, orthologues...

Ngày tải lên: 18/02/2014, 18:20

... distribution of DNA repair factors in situ in living cells has been providing us with striking information. For instance, even following exposure to ionizing radiation (IR), the DNA end -binding factor ... AT-rich S ⁄ MAR DNA of an anchor of chro- matin loops that tend to transform into ssDNA by unwinding [38] and are potential sites for aggregation of S ⁄ MAR -binding protein...

Ngày tải lên: 19/02/2014, 06:20

... small change in the isoelectric point, likely caused by the modification of a few amino acid residues upon binding of aminopentynal. This binding seems to be nonspecific, as the existence of some micro- heterogeneity ... (1971) The measurement of amino groups in proteins and peptides. Biochem. J. 124, 581–590. 28. Habeeb, A.F.S.A. (1966) Determination of free amino groups i...

Ngày tải lên: 19/02/2014, 16:20

... obtained by changing the Not1-Age 1 fragment of the pCV-UTR4 with the corresponding fragment deleted from all nucleotides of SLB1 T. Astier-Gin et al. Binding and replication of 3¢-end of HCV minus ... Secondary struc- ture of a fragment spanning from nt 229–341 in domain II (predicted by RNA DRAW soft- ware). Binding and replication of 3¢-end of HCV minus RNA T. Astier-Gi...

Ngày tải lên: 20/02/2014, 01:20

... ribozyme, binding of SG9 generates a duplex of only 5 bp due to the terminal G-A mis- match. Thus, the nonpaired adenosine residue at the hinge point can be integrated into the linker leading to further ... enhanced coaxial stacking of the two domains in the relevant variant of the hinged conventional hairpin ribozyme leading to enrichment of an extended inactive confor- matio...

Ngày tải lên: 20/02/2014, 01:20

... C-terminal tail of TAP2 induces a conformation in NBD2 that is nonpermissive for ATP binding. The ADP -binding state of TAP2 allows binding of ATP to TAP1. We hypothesize that peptide binding to ... nonpermissive for ATP binding. The resulting ADP -binding state of TAP2 and the C-terminal tail of NBD1 allow binding of ATP to TAP1. Peptide binding leads to a change...

Ngày tải lên: 20/02/2014, 02:21

... Peter, 3). We begin by creating a document S from the N-first web snippets so that each line of S con- tains a complete snippet. Each textline of S is then tagged with Part of Speech using the SVM- Tagger ... chun- ked in the next step. The chunker recognizes two types of word chains. Each chain consists of longest matching sequences of words with the same PoS class, namely noun ch...

Ngày tải lên: 20/02/2014, 05:20

... medium containing arabinose (lanes 1–3). For depletion of SecE (lanes 4–6), cells were grown in the absence of arabinose for 8 h. The cells were then induced with 1 m M IPTG for 10 min. Cells were ... with [ 35 S]methionine for 5 min and chased for 5 min either in the presence of arabinose to induce expression of SecE (lanes 1–3) or in the absence of arabinose to deplete...

Ngày tải lên: 20/02/2014, 11:20