Tài liệu Báo cáo khoa học: Mg2+-modulated KMnO4 reactivity of thymines in the open transcription complex reflects variation in the negative electrostatic potential along the separated DNA strands ppt
Tài liệu Báo cáo khoa học: Mg2+-modulated KMnO4 reactivity of thymines in the open transcription complex reflects variation in the negative electrostatic potential along the separated DNA strands ppt
... 2839
Mg
2+
-modulated KMnO
4
reactivity of thymines in the open
transcription complex reflects variation in the negative
electrostatic potential along the separated DNA strands
Footprinting of Escherichia coli ... (c) the observed thymine
reactivity and the magnitude of the Mg effect reflect variation in the
strength of the electros...
Tài liệu Báo cáo khoa học: DNA strand exchange activity of rice recombinase OsDmc1 monitored by fluorescence resonance energy transfer and the role of ATP hydrolysis pptx
... the latter. Interestingly, it appears that protein
binding to ssDNA in the absence of any nucleotide
cofactor results in attenuation of spontaneous anneal-
ing (compare line 1, Fig. 3 with line ... 2A). The
results are best rationalized by invoking better binding
of protein to ssDNA compared to dsDNA under these
conditions, thereby leading to suppression of ssDNA
renaturatio...
Tài liệu Báo cáo khoa học: Affinity and kinetics of proprotein convertase subtilisin ⁄ kexin type 9 binding to low-density lipoprotein receptors on HepG2 cells docx
... with tyrosines of the protein.
However, analysis in single-point binding assays showed
only 50–60% of the cell-associated [
125
I]PCSK9 could be
inhibited in the presence of a large excess of unlabeled
PCSK9, ... to
HepG2 cells. First, the number of LDLRs on HepG2
cells increased following growth in the absence of lipo-
proteins, and there was a corresponding incre...
Tài liệu Báo cáo khoa học: A DExD⁄ H box RNA helicase is important for K+ deprivation responses and tolerance in Arabidopsis thaliana docx
... conditions, the
greater induection of AKT1 expression at the transcrip-
tional level might have resulted in an increase in K
+
uptake or net K
+
-induced in ux. Taking the findings
together, this ... the helps mutant and OE lines of AtHELPS. (A) The conserved
motifs of DExD ⁄ H-box RNA helicases in AtHELPS. Numbers represent the amino acid position of the AtHELPS p...
Tài liệu Báo cáo khoa học: Functional and structural analyses of N-acylsulfonamidelinked dinucleoside inhibitors of RNase A ppt
... [46]. The similarity in the
K
m
values is attributable to the uracil moiety binding
in the same fashion [38], which could trigger the initial
binding of both substrates. In UpG, the binding of the
guanine ... contrast, the exocyclic
N
6
-amino group of adenine forms a hydrogen bond
with the side chain of Asn71, increasing the affinity of
RNase A for UpA. Thi...
Tài liệu Báo cáo khoa học: Active and regulatory sites of cytosolic 5¢-nucleotidase doc
... 2
Study of cN-II crystallized in the presence of adeno-
sine indicates that this nucleoside binds to this site,
even though only the amino acid residues involved in
the binding of the purine base ... [1]. The transfer of
phosphate can lead to phosphorylation of inosine,
guanosine and a number of their analogues [2]. There-
fore, in addition to being involved in r...
Tài liệu Báo cáo khoa học: Structural and functional studies of the human phosphoribosyltransferase domain containing protein 1 docx
... side chain of Lys173 interacts with both
O6 and N7 (Fig. 1C). The 2¢OH of the ribose is hydro-
gen bonded to the main chain and the side chain of
Asp142 (Fig. 1C). Both hydroxyl groups of the ribose
are ... for
the quantitation of microgram quantities of protein
utilizing the principle of protein-dye binding. Anal
Biochem 72, 248–254.
28 Page R & Stevens RC (20...
Tài liệu Báo cáo khoa học: A strategy for discovery of cancer glyco-biomarkers in serum using newly developed technologies for glycoproteomics ppt
... glycoproteins from the culture media and HCC patient
sera. Of these, 60 proteins were discarded, as they
were also identified from the sera of HVs. To estimate
the abundance of the remaining candidates ... robust clinical
endpoints, owing to their lack of specificity. In contrast,
the glycoprotein biomarkers identified by our strategy
may have the potential to be incorp...
Tài liệu Báo cáo khoa học: Identification and characterization of the transcription factors involved in T-cell development, t-bet, stat6 and foxp3, within the zebrafish, Danio rerio docx
... proteins, a leucine zipper domain
and a C2H2 zinc finger domain, both of which are
thought to help mediate DNA binding and may be
involved in the induction of dimerization [43]. Each of
these ... protein DNA- binding domain and an SH2
domain, which binds phosphorylated tyrosine residues
in the context of a longer peptide motif within a target
protein [38,39]. Within zebrafish...
Tài liệu Báo cáo khoa học: Post-translational modification of the deubiquitinating enzyme otubain 1 modulates active RhoA levels and susceptibility to Yersinia invasion pptx
... findings reveal a new aspect of the
complex interplay in host–pathogen interactions and
demonstrate a physiological role of the deubiquitinat-
ing enzyme OTUB1 in Yersinia invasion. OTUB1 as a
potential ... reports of
emerging multidrug resistant strains [3]. Pathogenic
Yersiniae are typically taken up through ingestion and
first reach the intestine. The Yersinia surface p...
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