Tài liệu Báo cáo khoa học: Endogenous expression and protein kinase A-dependent phosphorylation of the guanine nucleotide exchange factor Ras-GRF1 in human embryonic kidney 293 cells pptx

... 2304–2316 ª 2005 FEBS Endogenous expression and protein kinase A-dependent phosphorylation of the guanine nucleotide exchange factor Ras-GRF1 in human embryonic kidney 293 cells Jens Henrik Norum 1 , ... report the endogenous expres- sion of Ras-GRF1 in HEK293 cells. Serotonin stimulation of HEK293 cells transiently expressing G s -couple...

Ngày tải lên: 19/02/2014, 18:20

... restored the bacterial protein [21,33]. Interestingly, the same difficulty in stripping off the metallocofactor from the protein has been described for murine adenosine deaminase, a member of the amidohydrolase ... structures of human and bacterial ACMSD, demonstrating that the region surrounding residues involved in catalysis is more hydrophobic in the human,...

Ngày tải lên: 19/02/2014, 02:20

... substitution of glycine residues by alanine or proline and the exchange of proline by glycine. Glycine residues are frequently found at the C-terminal ends of a helices providing the conforma- tional ... activity The enzymatic activity of the recombinant protein was examined in FLS incubations employing dihydroquercetin or dihydrokaempferol as a substrate. Both these...

Ngày tải lên: 21/02/2014, 03:20

... protein (CBP) [23], a peptide from the Win motif of MLL1 bound to the WD40 repeat protein, WDR5 [24,25] and the C-terminal SET domain in the presence and absence of histone peptides and the cofactor ... coordinates two zinc ions using the two conserved CGXCXXC motifs (Fig. 2A). The zinc ions are required for the structural integrity of the protein, as mutat...

Ngày tải lên: 16/02/2014, 14:20

... was examined on K562 human erythroleukemic cells and A431 human epidermoid carcinoma cells by measuring the incorporation of [ 35 S]methionine into newly synthesized proteins after 96 h of incubation ... simultaneously interacts with the main phosphate of the substrate [32]. The closer positioning of the N-terminus to the main protein body is essential for the...

Ngày tải lên: 19/02/2014, 12:20

... 18.7 A ˚ on binding of cAMP to the anti-cAMP-binding sites of the protein. The distance between the sulfur atom of Cys178 and the C9–C10 bond of the indole ring of Trp85, derived from the crystal ... absence of cAMP in apo-CRP, some b strands of the N-terminal domain of the protein may collapse into the cAMP-binding pocket, causing reorientation of...

Ngày tải lên: 20/02/2014, 23:20

... of the input language and the primitives of the data base, which makes it possible to translate input questions into query expressions in a rather straightforward fashion. In designing the ... independently of each other (one on the basis of the possible Eng- lish questions about the subject-domain, the other on the basis of the structure of the d...

Ngày tải lên: 21/02/2014, 20:20

... [35] may not be considered in the light of the results obtained in the present study. Rather, the involvement of manganese in the in vivo assembly of the metallocofactor of C. ammoniagenes R2F is ... 37 °C and then plated for selection of transformants. Protein techniques Protein was determined by protein- dye binding with BSA as a standard [47]. Whole cel...

Ngày tải lên: 15/02/2014, 01:20

... located in the sub- unit contact area of the tetramer, whereas the remaining two mismatches sur- round the contact area; this is expected to manifest in the kinetic and thermodynamic properties of the ... different kinetic and thermodynamic properties of the tetrameric enzyme may result in fine-tuning of the properties of skeletal LDH-A, which is in line w...

Ngày tải lên: 19/02/2014, 02:20

... coordinated changes in the expression of the transferrin receptor and ferritin; (b) developed iron accumulation and (c) increased the prooxidant state and the level of oxidatively modified proteins. ... in the cytoplasm of human epithelial cells of the gastrointestinal tract and liver, two central organs in the regulation of iron metabolism [20,21]....

Ngày tải lên: 19/02/2014, 16:20