Tài liệu Báo cáo khoa học: Mammalian mitotic centromere-associated kinesin (MCAK) A new molecular target of sulfoquinovosylacylglycerols novel antitumor and immunosuppressive agents pptx

... Mammalian mitotic centromere-associated kinesin (MCAK) A new molecular target of sulfoquinovosylacylglycerols novel antitumor and immunosuppressive agents Satoko Aoki 1,2 , Keisuke Ohta 1 , ... membrane was stained with anti-His 6 Ig and alkaline phosphatase. A single band was present that corresponded to the molecular mass of MCAK184 (49 kDa). S. Aok...

Ngày tải lên: 19/02/2014, 17:20

... following apoptotic stimuli results in the release of cytochrome c and subsequent activation of caspase-9 and caspase-3, which are cystein proteases that cleave vital cellular targets and cause apoptosis. ... (Iressa). Mol Cancer Ther 2, 345–351. 61 Adam L, Vadlamudi R, Mandal M, Chernoff J & Kumar R (2000) Regulation of microfilament reorgani- zation and invasiveness of br...

Ngày tải lên: 16/02/2014, 09:20

... MINIREVIEW Marine toxins and the cytoskeleton: a new view of palytoxin toxicity M. Carmen Louzao, Isabel R. Ares and Eva Cagide Departamento de Farmacologia, Facultad de Veterinaria, Universidad de Santiago ... Ishida S, Inoue A, Kan Y & Yasumoto T (1995) Palytoxin analogs from the dino- flagellate Ostreopsis siamensis. J Am Chem Soc 117, 5389–5390. 5 Taniyama S, Arakawa O, Tera...

Ngày tải lên: 18/02/2014, 14:20

... Taq DNA polymerase (Promega, Madison, WT, USA) using the primers 5¢-CACACTACACTGGGAAGCAGAG ACTCCAGC-3¢ and 5¢-AGCTGGCCCAGCAGCCATACACAGTTAAAG- 3¢. The cDNA was subcloned into the EcoRV site of ... Tomohiro Chiba for preparation of embryonic day 9.5 mouse embryos; Dr Dovie Wylie and Ms Tak- ako Hiraki for expert assistance; and all members of the Departments of Pharmacology and...

Ngày tải lên: 18/02/2014, 16:20

... of the Y chromosome of the medaka, Oryzias latipes. Proc Natl Acad Sci USA 99, 11778–11783. 9 Matsuda M, Nagahama Y, Shinomiya A, Sato T, Matsuda C, Kobayashi T, Morrey CE, Shibata N, Asakawa ... Suzuki A, Kobayashi T, Paul-Prasanth B, Lau EL, Hamaguchi S, Sakaizumi M & Nagahama Y (2007) DMY gene induces male development in genetically female (XX) medaka fish. Proc Natl Acad Sci USA...

Ngày tải lên: 14/02/2014, 19:20

... K, Hieda N, Yamanishi M, Shibata N & Toraya T (2005) Crystallization and preliminary X-ray analysis of molecular chaperone-like diol dehydratase- reactivating factor in ADP-bound and nucleotide-free forms. ... or apoenzyme was used (data not shown). If the a, b and c subunits of the enzyme are abbreviated as a D , b D , and c D , respectively, and the a and b subuni...

Ngày tải lên: 15/02/2014, 01:20

... IV) antibody to normalize for loading. Statistical analysis All values are expressed as mean ± standard deviation. Statistical significance was determined using a one-way HSS and mitochondrial ... substance. Am J Physiol 242, G289–G295. 4 Tanigawa K, Sakaida I, Masuhara M, Hagiya M & Okita K (2000) Augmenter of liver regeneration (ALR) may promote liver regeneration by reducing natur...

Ngày tải lên: 16/02/2014, 09:20

... 5¢-GGAGATCTCCAGTGA TATCGACCA-3¢ and 5¢-ACGGCTTCTACGGATCGAA ACT-3¢ for PPARc ,5¢-AAGACAGCTCCTCCTCGAAGG TT-3¢ and 5¢-TGACCAAATCCCCATTTACGC-3¢ for aP2, 5¢-ATCCATGGATGGACGGTAACG-3¢ and 5¢-CTGGA TCCCAATACTTCGACCA-3¢ ... 5¢-GCGTCGGGTAGATCCAGTT-3¢ and 5¢-CTC AGTGGGGCTTAGCTCTG-3¢ for ACC, and 5¢-AACAC CCCAGCCATGTACGTAG-3¢ and 5¢-TGTCAAAGAAA GGGTGTAAAACGC-3¢ for b-actin. Expression levels...

Ngày tải lên: 16/02/2014, 09:20

... are characterized by a conserved array of 20 cysteines and the absence of His and aromatic amino acids. MT3 contains 68 amino acids with 70% sequence identity to the MT1 and MT2 (MT1 ⁄ 2) isoforms. ... kinetically labile, allowing rapid intra- molecular and intermolecular metal transfer. This is a direct consequence of the relatively high structural dynamics and flexibili...

Ngày tải lên: 16/02/2014, 15:20

... eliminate several state variables). Such an elimination yields a system of non- linear algebraic equations; analytic solutions of such systems are typically unattainable. Hence, high values of m and/ or ... same order of magnitude. In all of these cases, the accuracy of Michaelis–Menten kinetics is unsatisfactory as a result of small timescale separation. Particular exam- p...

Ngày tải lên: 18/02/2014, 06:20