Tài liệu Báo cáo khoa học: Guanidinium chloride- and urea-induced unfolding of FprA, a mycobacterium NADPH-ferredoxin reductase Stabilization of an apo-protein by GdmCl docx

... Guanidinium chloride- and urea-induced unfolding of FprA, a mycobacterium NADPH-ferredoxin reductase Stabilization of an apo-protein by GdmCl Nidhi Shukla 1 , Anant Narayan Bhatt 1 , Alessandro ... unfolding of FprA in urea and GdmCl suggests dramatically different pathways and mechanism for the two denaturants as summarized in Fig. 6. The urea-ind...

Ngày tải lên: 19/02/2014, 16:20

... surface. Data analysis All binding and kinetic data were fitted by nonlinear regres- sion with prism 5 (GraphPad Software, CA, USA). For inhibition-binding curves, the raw data were analyzed according ... two-exponential association model. k obs(rapid) and k obs(slow) are the observed association rate constants for the rapid and slow association phases, respectively. The values for the...

Ngày tải lên: 14/02/2014, 14:20

... 213–222. 66 Hamaguchi M, Hamada D, Suzuki KN, Sakata I & Yanagihara I (2008) Molecular basis of actin reorgani- zation promoted by binding of enterohaemorrhagic Escherichia coli EspB to alpha-catenin. ... Y, Sagara H, Kabe Y, Azuma M, Kume K, Ogawa M, Nagai T, Gillespie PG, Sasakawa C & Handa H (2007) The enteropathogenic E. coli effector EspB facilitates microvillus effacing a...

Ngày tải lên: 14/02/2014, 22:20

... CCATGTCTGCAGATGGTCGAGG 5¢-RACE Zf5¢stat6-R2 GGACTGACATTGCTCCAGAGC 5¢-RACE Zf3¢stat6-F3 GCTTCAGTGACTCAGAAATTGG 3¢-RACE Zf3¢stat6-F4 GTCCAGAATATTCAGCCTTTCACC 3¢-RACE Zftbet-F1 CTCCCTCAAACAAACCAGAGTC Initial PCR Zftbet-R1 ... CGAGCAGGAGATGGGAACC Real-time PCR Zfbactin-R CAACGGAAACGCTCATTGC Real-time PCR Zfgapdh-F CGCTGGCATCTCCCTCAA Real-time PCR Zfgapdh-R TCAGCAACACGATGGCTGTAG Real-time PCR ZFil...

Ngày tải lên: 16/02/2014, 09:20

... 5¢-GGAGATCTCCAGTGA TATCGACCA-3¢ and 5¢-ACGGCTTCTACGGATCGAA ACT-3¢ for PPARc ,5¢-AAGACAGCTCCTCCTCGAAGG TT-3¢ and 5¢-TGACCAAATCCCCATTTACGC-3¢ for aP2, 5¢-ATCCATGGATGGACGGTAACG-3¢ and 5¢-CTGGA TCCCAATACTTCGACCA-3¢ ... 5¢-GCGTCGGGTAGATCCAGTT-3¢ and 5¢-CTC AGTGGGGCTTAGCTCTG-3¢ for ACC, and 5¢-AACAC CCCAGCCATGTACGTAG-3¢ and 5¢-TGTCAAAGAAA GGGTGTAAAACGC-3¢ for b-actin. Expression levels...

Ngày tải lên: 16/02/2014, 09:20

... adrenergic and cholinergic in nature, and originate from paravertebral sympathetic ganglia [16]. Experimental nerve deletion and immunohisto- chemistry analysis have shown that both myelinated and unmyelinated ... Baldock PA & Sainsbury A (2010) Neuropeptide Y and sex hormone interactions in humoral and neuronal regulation of bone and fat. Trends Endocrinol Metab 21, 411...

Ngày tải lên: 18/02/2014, 04:20

... and wash fractions was quantitated by scintillation counting. For MSR-catalysed assays (used for measuring the K m of NADPH and the K act of MSR), AqCbl and dithiothreitol were replaced by varying ... enzyme-bound FAD to the FMN centre, and ultimately to an acceptor redox pro- tein or domain. Although the bacterial FNR ⁄ Fld and mammalian MSR are not interchangeable in rea...

Ngày tải lên: 18/02/2014, 08:20

... (TaKaRa Bio Inc.). Forward primers 5¢-CG TTTGAAGGGTGAGGAGGAAAA[FAM]G-3¢ and 5¢-CA CAAGAGAGTTCTGCGATAACCTTG[FAM]G-3¢ (Invi- trogen Corporation) and reverse primers 5¢-AAGTAGGCA ACAAAACAACG-3¢ and ... 77–99. 16 Kamauchi S, Wadahama H, Iwasaki K, Nakamoto Y, Nishizawa K, Ishimoto M, Kawada T & Urade R (2008) Molecular cloning and characterization of two soybean protein disulfide iso...

Ngày tải lên: 18/02/2014, 11:20

... SREBP-1c by saturated fatty acids and PU- FAs. Saturated fatty acids activate, and PUFAs suppress, SREBP- 1c, leading to and protecting from hepatic insulin resistance and pancreatic b-cell insulin ... defined. SREBP and parasympathetic function in heart Parasympathetic stimulation of the heart involves activation of GIRK1 ⁄ 4, a G-protein-coupled inward- rectifying potassium...

Ngày tải lên: 18/02/2014, 13:20

... 926–934. 19 Yano Y, Sakon M, Kambayashi J, Kawasaki T, Senda T, Tanaka K, Yamada F & Shibata N (1995) Cytoskel- etal reorganization of human platelets induced by the protein phosphatase 1 ⁄ 2 A inhibitors ... of stabilization of focal adhesions and a consequent loss of cytoskeletal organization due to an alteration in the tyrosine-phosphory- lated state of the focal...

Ngày tải lên: 18/02/2014, 14:20