Tài liệu Báo cáo khoa học: The phosphatase activity of the i

Tài liệu Báo cáo khoa học: The phosphatase activity of the isolated H4-H5 loop of Na+/K+ ATPase resides outside its ATP binding site docx

... at the ATP and pNPP sites on the N-domain of the H 4 -H 5 loop of Na + /K + ATPase. The models u sed for (A–D ) are the same a s in Fig. 5. (A) and (C) This comparison of pNPP binding to the ATP ... revealed that the H 4 -H 5 loop may contain separate binding sites for ATP and pNPP (Figs 5 and 6). Within the isolated H 4 -H 5 loop, the ATP site o...

Ngày tải lên : 19/02/2014, 16:20

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Tài liệu Báo cáo khoa học: An alternative isomerohydrolase in the retinal Muller cells of a cone-dominant species doc

... that the enzymatic activity of RPE65c is iron-dependent. Characterization of the kinetic parameters of the enzymatic activity of zebraﬁsh RPE65c To determine the steady-state kinetics of the ... thetase to esterify the 13cROL generated in the reaction. Therefore, 13cROL is accumulated in the absence of LRAT in the reaction (Fig. S3B). Under the in vitro...

Ngày tải lên : 14/02/2014, 14:20

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Tài liệu Báo cáo khoa học: TEC family kinases in health and disease – loss-of-function of BTK and ITK and the gain-of-function fusions ITK–SYK and BTK–SYK pptx

... posi- tioned in the connecting loops. Approximately one- third of all mutations in the BTK gene are missense and some of these reduce the stability of the protein. This is exempliﬁed by mutations in the ... identify individuals with mild disease, thereby providing the missing data. The phenotype of XLA and the potential of BTK and ITK inhibitors The outcome of de...

Ngày tải lên : 14/02/2014, 18:20

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Tài liệu Báo cáo khoa học: Interaction between very-KIND Ras guanine exchange factor and microtubule-associated protein 2, and its role in dendrite growth – structure and function of the second kinase noncatalytic C-lobe domain docx

... that the core sequence critical for the speciﬁc MAP2 binding resides within the 32 residues (amino acids 456–487) of the v-KIND KIND2 domain. To examine whether the MAP2 binding core site of v-KIND ... lysates. Taken together, these data indicate that the MAP2- binding core site of v-KIND is sufﬁcient for the v-KIND-MAP2 interaction within cells. The MAP2 -bi...

Ngày tải lên : 14/02/2014, 19:20

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Tài liệu Báo cáo khoa học: Steﬁn A displaces the occluding loop of cathepsin B only by as much as required to bind to the active site cleft doc

... termed the ‘occluding loop , occupies the part of the active site cleft on the primed side and blocks access to the active site cleft beyond the S2¢ substrate binding site [11,12]. The occluding loop ... form of cathepsin B clearly exposed the relevance of the occluding loop for the exopeptidase activity [11], they do not explain the mechanisms of...

Ngày tải lên : 18/02/2014, 04:20

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Tài liệu Báo cáo khoa học: Toggle switches, pulses and oscillations are intrinsic properties of the Src activation/deactivation cycle doc

... presenting the pulse of Src activity in the plane of the inactive and active fractions, s i and s 1 . If the duration of the stimulus exceeds the critical value, the trajectory in the (s i , ... can bind the Src SH2 domain, facilitating dephosphorylation of the inhibitory tyrosine pY i . The phosphatases that dephosphorylate the activating site pY a include...

Ngày tải lên : 18/02/2014, 11:20

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Tài liệu Báo cáo khoa học: A facile method for expression and puriﬁcation of the Alzheimer’s disease-associated amyloid b-peptide pdf

... understanding of the structure of both the Ab monomer and its various assemblies could help in the design of new therapeutic strategies targeted at preventing the formation or ameliorating the activity of ... concentrated manner at the top of the column, or, if bound to the resin prior to packing the column, the salt gradient concen- trates the peptide on...

Ngày tải lên : 18/02/2014, 13:20

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Tài liệu Báo cáo khoa học: Verprolin function in endocytosis and actin organization Roles of the Las17p (yeast WASP)-binding domain and a novel C-terminal actin-binding domain doc

... 5A,B). Loss of the LBD abolished the ability of C-Vrp1p 364)817 to restore growth of vrp1D cells at elevated temperature. We next assessed the importance of the LBD for the ability of C-Vrp1p 364)817 to ... mutations in the N-terminal actin -binding WH2 domain (WH2-1) or even deletion of the entire N-terminal module of Vrp1p did not signiﬁcantly perturb the act...

Ngày tải lên : 18/02/2014, 16:20

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Tài liệu Báo cáo khoa học: Protein kinase CK2 activates the atypical Rio1p kinase and promotes its cell-cycle phase-dependent degradation in yeast pdf

... suggesting the absence of CK2 sites N-terminal of position 402 in the catalytic domain and the presence of several phosphorylation sites for CK2 in the C-terminal portion of Rio1p, one (or more) of them ... depletion experiments or the use of the weak active site mutant of Rio1 (D244E) in which inhibition of the entrance of ana- phase was the most signiﬁcan...

Ngày tải lên : 18/02/2014, 16:20

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Tài liệu Báo cáo khoa học: Metabolic gene switching in the murine female heart parallels enhanced mitochondrial respiratory function in response to oxidative stress pdf

... reaction of the luciferin ⁄ luciferase mixture, energy donor (ATP) and oxygen results in the emission of light. The intensity of the bioluminescence was detected using a luminometer, and the mitochondrial ... were approved by the University of Cape Town’s Animal Research Ethics Committee, and the investigation conforms to the Guide for the Care and Use of Laborator...

Ngày tải lên : 18/02/2014, 16:20

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