Tài liệu Báo cáo khoa học: Biochemical characterization of Bacillus subtilis type II isopentenyl diphosphate isomerase, and phylogenetic distribution of isoprenoid biosynthesis pathways doc

... of Bacillus subtilis type II isopentenyl diphosphate isomerase, and phylogenetic distribution of isoprenoid biosynthesis pathways Ralf Laupitz 1 , Stefan Hecht 1 , Sabine Amslinger 1 , Ferdinand Zepeck 1 , ... S1. Isoprenoid biosynthesis in completed and unfin- ished prokaryotic genomes. Ó FEBS 2004 B. subtilis type II IPP isomerase (Eur. J. Biochem. 2...

Ngày tải lên: 19/02/2014, 13:20

... Peak 3 produced small amounts of HMG-CoA and large amounts of free CoA. Peak 4 produced HMG-CoA and also large amounts of free CoA. Peak 5 produced large amounts of HMG-CoA, but also free CoA. Human ... 2006 FEBS Biochemical characterization of human 3-methylglutaconyl-CoA hydratase and its role in leucine metabolism Matthias Mack 1 , Ute Schniegler-Mattox 2 , Verena Peter...

Ngày tải lên: 19/02/2014, 07:20

... the NRE wild -type [36], NRE mutants [33], FAS-2 wild -type and CLV-1 were synthesized and annealed. Each annealed pair of oligonucleotides possess a 32 bp sequence of a 3¢ UTR region of transcripts ... that of other well- characterized members of PUF family. These proteins must bind to transcripts in the plant and regulate their translation and ⁄ or turnover. A large numb...

Ngày tải lên: 18/02/2014, 06:20

... expressed, and purified in Escherichia coli system. The enzymatic characterization of HpSDH demonstrates its activity with k cat of 7.7 s )1 and K m of 0.148 mm toward shikimate, k cat of 7.1 s )1 and ... catalytic proper- ties of HpSDH and the effects of pH and temperature on HpSDH. The results show that HpSDH has a k cat of 7.7 ± 0.9 s )1 , K m of 0.148 ± 0.028 mm...

Ngày tải lên: 19/02/2014, 05:20

... of monoacetylacetoatoiron(III) and the effect of copper (II) on the formation of monothenoyltrifluoroacetonatoiron (III). Inorg Chem 10, 2096–2101. 34 Straganz GD, Hofer H, Steiner W & Nidetzky ... I. Identification of zinc finger proteins. AIP Conf Proc 882, 322–324. 33 Fay DP, Nichols AR & Sutin N (1971) The kinetics and mechanisms of the reactions of iron(III) with b-d...

Ngày tải lên: 18/02/2014, 06:20

... FEBS Kinetic characterization of the first step of the ribozyme-catalyzed trans excision-splicing reaction P. Patrick Dotson II* , Joy Sinha* and Stephen M. Testa Department of Chemistry, University of Kentucky, ... constant of the 5¢ exon mimic, h is the fraction of 5¢ exon mimic bound to the ribozyme, and [ribozyme] u is the concentration of unbound ribozyme in the react...

Ngày tải lên: 18/02/2014, 18:20

... 3¢-end of Oligo II could be ligated to the BstEII site. Similarly, the 5¢-ends of Oligos III, V, VII and IX were designed to ligate to the BstEII, BglII, EagI and NcoI sites, respectively, and their ... form of rat HO-1 [37], H64L mutant of myoglobin [38], maize ferredoxin type III [39], maize FNR [40], a truncated form of human CPR [41], and rat BVR [42]. Heme binding...

Ngày tải lên: 19/02/2014, 05:20

... changes in ASA of the apolar and polar residues, respectively [39]. The structure-based calculations provide a DC p of )407 calÆmol )1 ÆK )1 and DASA apolar and DASA polar of )1354 and )777 A ˚ 2 , ... form and structured in the bound form. Knowledge of the dynamic characteris- tics of both IL-8 and N-domain before and after binding and whether binding is accompanied...

Ngày tải lên: 19/02/2014, 05:20

... consisting of four copies of four subunits, a, b, c and d. The a and b subunits are regu- latory and are the sites of phosphorylation and meta- bolite binding and are also regulated by the binding of ... (1992) High and intermediate affinity calmodulin binding domains of the alpha and beta subunits of phosphorylase kinase and their potential role in phosphorylation-...

Ngày tải lên: 19/02/2014, 17:20

... UK; 2 Department of Chemistry, University of Otago, Dunedin, New Zealand; 3 Department of Neurology, Medical School, University of Newcastle upon Tyne, UK The selective manipulation of the expression and ... mitochondria and cells. In addition to facilitating the development of antigenomic therapies, such a reagent might also be useful in investi- gating the rate of turnover...

Ngày tải lên: 20/02/2014, 11:20