Tài liệu Báo cáo khóa học: Quantitative analysis, using MALDI-TOF mass spectrometry, of the N-terminal hydrolysis and cyclization reactions of the activation 2 process of onconase pdf

... the presence of 3 M guanidinium chloride. 1166 M. Ribo ´ et al. (Eur. J. Biochem. 27 1) Ó FEBS 20 04 Quantitative analysis, using MALDI-TOF mass spectrometry, of the N-terminal hydrolysis and cyclization ... (Met1)-ONC (M23L) by the addition of AAP, as described in the Materials and methods. Completion of the hydrolysis was confirmed by the disappe...

Ngày tải lên: 19/02/2014, 12:20

... raised. The effect of DCMU on the traces was to increase the amplitude of step J with the concurrent decline of step I, and to retard the rise to F m . With dPQ, the J step was lowered and the subsequent ... depends on the relative amplitude and rate constant of the J–I phase compared to the amplitudes and rate constants of the two other phases. The a...

Ngày tải lên: 19/02/2014, 05:20

... F b P b Observed change RPLP2 P05387 11.7 4. 42 4 26 8 53 8.41 0. 020 DA fl·3.9 Parathymosin P209 62 11.4 4.14 2 88 22 12. 14 0.008 DA fl 2. 3 eIF5A isoform B P6 324 1 16.8 5.08 2 70 7 12. 72 0.007 DA fl 2. 1 L7 ⁄ L 12, mitochondrial ... mitochondrial P 528 15 21 .4 5.37 3 140 11 9 .28 0.016 DA fl·1.6 Peroxiredoxin 1 Q06830 22 .1 8 .27 9 431 45 10.39 0.0 12 DA ›·1.5 Annexin A5 Q6FHB3...

Ngày tải lên: 15/02/2014, 01:20

... [S] cross . Modulation of butyrylcholinesterase catalytic activitiy P. Masson et al. 26 20 FEBS Journal 27 5 (20 08) 26 17 26 31 ª 20 08 The Authors Journal compilation ª 20 08 FEBS both the competitive inhibition of hydrolysis ... catalytic activitiy P. Masson et al. 26 28 FEBS Journal 27 5 (20 08) 26 17 26 31 ª 20 08 The Authors Journal compilation ª 20 08 FEBS terms...

Ngày tải lên: 18/02/2014, 17:20

... (kJÆmol )1 ) CuTMPyP 20 .0 1.01 6.36 ) 27 .40 H 2 TMPyP 20 .0 0.99 4.49 ) 26 .55 CuTCPP 32. 2 0.97 2. 97 ) 25 .53 H 2 TCPP 48 .2 1.03 2. 84 ) 25 . 42 ZnTPPS 65.6 1. 02 1.10 ) 23 .07 H 2 TPPS 34 .2 1.05 0.58 ) 21 .48 Table 2. ... 90.8 25 (6.80) 30 4.35 H 2 TMPyP 20 6.60 25 4.49 ) 59.5 ) 110.8 30 2. 67 35 2. 10 35 (2. 15) CuTCPP 10 25 . 32 15 10 .26 20 5.85 25 2...

Ngày tải lên: 19/02/2014, 16:20

... to the Dp 427 isoform of dystroph in (A), the a-subunit of the d ystroglycan complex ( a-DG; B), the N a + /K + ATPase (N KA, C), the fast SERCA1 i soform of the sarcoplasmic r et iculum Ca 2+ ATPase (D), ... t he 2D ÔStains- AllÕ analysis of Fig. 3 and the 1D i mmunoblotting of Fig. 4. As the full-length Dp 427 isoform of dystrophin does not enter the second...

Ngày tải lên: 19/02/2014, 16:20

... genes, including Dec2 and PPARc2,intheabsence of other transcription facto rs, such as CLOCK and BMAL1 [9,10 ,22 ,23 ]: an N-terminal region between amino acids 1 and 141, and another region between ... ility for the E-box (lane 7), and substitution of Arg65 for Ala(DEC1-R65A and DEC1:4– 23 32- R65A) abolished DEC1 binding to the E-box (lanes 6 and 16). On t he other...

Ngày tải lên: 19/02/2014, 16:20

... 1186–1191. 23 02 FEBS Journal 27 2 (20 05) 22 92 23 03 ª 20 05 FEBS Control analysis of the las operon B. Koebmann et al. on the TP901-1 attB locus on the chromosome of MG1363. The replication-thermosensitive ... Koebmann et al. 22 96 FEBS Journal 27 2 (20 05) 22 92 23 03 ª 20 05 FEBS AB Fig. 6. Modulation of the las operon. (A) Enzyme activities of PFK, PK and LD...

Ngày tải lên: 19/02/2014, 17:20

... mutant 2. 17 0.0 42 ± 0.001 Fru1,6P 2 RK9117 mutant 4.05 ± 0. 72 0.0 92 ± 0.001 Quantitation of channeling in intact E. coli G. Shearer et al. 326 2 FEBS Journal 27 2 (20 05) 326 0– 326 9 ª 20 05 FEBS the ... [ 14 C]Fru1,6P 2 to 14 CO 2 in the presence of [ 12 C]glucose. The data in Figs 2 and 3, considered separately, are insufficient to determine the predicted...

Ngày tải lên: 19/02/2014, 18:20

... of the "determining" words which will enable us to fix the semantic values of the "determineеs". The result of the aggregate of structural comparisons of this kind, and ... governs the genitive case. The task of deciding whether or not to retain the &apos ;of& apos; falls upon the reader. The problem results, of course, from the s...

Ngày tải lên: 19/02/2014, 19:20