Tài liệu Báo cáo khóa học: Cloning and characterization of two distinct isoforms of rainbow trout heat shock factor 1 ppt

... upregulation of transcripts of either of the genes (Fig. 5N and O, second lane). PsCBL B D F H J L O 18 S 1. 0 kb 18 S 1. 0 kb 18 S 1. 0 kb 18 S 1. 0 kb 18 S 1. 0 kb 18 S 1. 0 kb 18 S 1. 0 kb PsCIPK A C E G I K N 18 ... S 1. 0 kb PsCIPK A C E G I K N 18 S 1. 8 kb 18 S 1. 8 kb 18 S 1. 8 kb 18 S 1. 8 kb 18 S 1. 8 kb 18 S 1. 8 kb 18 S 1. 8 kb CaCl 2 SALICYLI...

Ngày tải lên: 19/02/2014, 07:20

... for existence of two distinct HSF1 isoforms in rainbow trout and the formation of heterotrimers of these isoforms in vitro. Materials and methods Cell culture and animals Rainbow trout gonadal ... encode distinct isoforms of HSF. Clone C1 was a partial cDNA containing an insert of 983 nucleotides Ó FEBS 2004 Rainbow trout HSF1 (Eur. J. Biochem. 2 71) 70...

Ngày tải lên: 19/02/2014, 12:20

... lectin (LD 50 5 10 lgÆkg )1 ) [19 ], ricin (IC 50 0.0 01 nm and LD 50 2.6 lgÆkg )1 ) [20] and abrin (IC 50 0.0037 nm and LD 50 0.56 lgÆkg )1 ) [ 21] . Agglutination and carbohydrate-binding of the B-chains It ... well, 10 0 lL of each sugar solution (0 .1 m) was placed and 50 lL was taken and serially two- fold diluted in wells con- taining 50 lL of NaCl ⁄ P i . Ne...

Ngày tải lên: 18/02/2014, 16:20

... ins number: Na + K + ATPase EEA1 Calnexin 12 345678 910 111 213 14 40 60 80 10 0 12 0 Grb14 control i2i *** * * 10 0 12 0 0 20 1 2 3 4 5 6 7 8 9 10 11 12 13 14 ins 2 min ins 5 min Fraction number * 0 20 40 60 80 12 34 6 8 910 111 213 14 * *** * * *** *** 12 345678 910 111 213 14 Fig. ... receptor and Grb7. A role for two of its binding domains, PIR and SH2. Oncogene 1...

Ngày tải lên: 18/02/2014, 18:20

... 10 50 91 139 75 Kojic acid 10 99 10 0 19 5 98 b-Mercaptoethanol 10 10 0 10 0 1 100 10 0 SDS 10 68 73 13 9 44 Benzaldehyde 10 64 42 13 0 13 Glutathione 10 18 10 0 1 17 10 0 NaCl 10 0 15 49 10 1 0 EDTA 10 ... Ichisima E (19 95) Molecular clon- ing and nucleotide sequence of the protyrosine gene, melO from Aspergillus oryzae and expression of the gene in yeas...

Ngày tải lên: 19/02/2014, 06:20

... (mU) 5 °C 0 19 .6 ± 0.37 2 .13 ± 0.22 9.25 ± 0.37 5 17 .7 ± 0.23 a 4.70 ± 0.50 a 4.72 ± 0 .13 a 10 18 .9 ± 0.37 10 .7 ± 0.64 a,b 1. 92 ± 0. 31 a,b 37 °C 0 12 7.0 ± 6.6 1. 29 ± 0 .19 7.23 ± 0. 51 10 53.7 ± ... range of the plots were 10 3.2 ± 0.2 and 80.0 ± 1. 2 kJ mol )1 in the absence and presence of phosphate. For the 15 –45 °C temperature range, the Ea values were 2...

Ngày tải lên: 19/02/2014, 16:20

... )25 .1 51. 7 ± 0.2 8 17 7 ± 5 76.2 ± 0.5 )25.6 60.7 ± 0.2 9 17 4 ± 6 87 .1 ± 0.9 )26.0 69.6 ± 0.2 10 17 2 ± 6 11 0 ± 5 )29.6 78.5 ± 0.3 a Expressed per mol of monomer. b k 1 units are mol –(n )1) Æmin )1 . Ó ... ANS, 8-anilino -1- naphthalenesulfonic acid. (Received 21 January 2002, revised 14 June 2002, accepted 11 July 2002) Eur. J. Biochem. 269, 412 1– 413 3 (2002) Ó FEBS...

Ngày tải lên: 21/02/2014, 03:20

... members IL -19 AAG16755 18 .3 16 .0 IL-20 NP_0 611 94 21. 9 19 .0 IL-22 AAK62468 14 .0 13 .8 IL-24 AAG 414 01 12.9 14 .3 IL-26 NP_060872 16 .9 15 .0 4650 R. Savan et al. (Eur. J. Biochem. 270) Ó FEBS 2003 and 3 min ... chemokines, IL-1b [11 ], tumor necrosis factor- a [12 ], transforming growth factor [13 ,14 ], IL-8 [15 ] andIFN [16 ],haveallbeenclonedinfish. Recently, IL -10 ho...

Ngày tải lên: 20/02/2014, 02:21

... ursula.kummer@bioquant. uni-heidelberg.de (Received 10 January 2 011 , revised 31 May 2 011 , accepted 15 June 2 011 ) doi :10 .11 11/ j .17 42-4658.2 011 .08 217 .x Systems biology has received an ever increasing ... (2003) Biotechnol Prog 19 , 11 36 11 41. 44 Carbohydrate, xylose catabolism Candida mogii Xylitol production is optimal for a substrate of 10 % glucose and 90% xylito...

Ngày tải lên: 14/02/2014, 14:20

... 8639 E-mail: p.n.cockerill@leeds.ac.uk (Received 18 December 2 010 , revised 10 February 2 011 , accepted 5 April 2 011 ) doi :10 .11 11/ j .17 42-4658.2 011 .0 812 8.x Chromatin is by its very nature a repressive ... Nature 466, 11 29 11 33. 214 Cannon SV, Cummings A & Teebor GW (19 88) 5-Hydroxymethylcytosine DNA glycosylase activity in mammalian tissue. Biochem Biophys Res Commun...

Ngày tải lên: 14/02/2014, 18:20