Tài liệu Báo cáo khóa học: The unusual methanogenic seryl-tRNA synthetase recognizes tRNASer species from all three kingdoms of life pptx

... The unusual methanogenic seryl-tRNA synthetase recognizes tRNASer species from all three kingdoms of life Silvija Bilokapic1,2, Dragana Korencic2,3, ... unpublisheddata). One of these enzymes is bacteria-like SerRSs, while the other is atypical archaeal SerRS [16], which is onlymarginally related to the homologues in nonmethanogenic species and outside the archaeal ... while the number of unpaired nucleotides at the base of variable arm reﬂects the similarity to eukaryotictRNAs, due to the presence of at least one unpairednucleotide between the possible stem of...

Tài liệu Báo cáo khoa học: The Arabidopsis protein kinase Pto-interacting 1-4 is a common target of the oxidative signal-inducible 1 and mitogen-activated protein kinases docx

... forms of the MAPKs were produced as GST-lofMPK3 and GST-lofMPK6. However, when lofMPK3 and lofMPK6proteins were tested for phosphorylation with OXI1,no phosphorylation of lofMPK3 or lofMPK6 ... [17]. On the otherhand, expression of the tomato SlPti1 cDNA in the rice Ospti1a mutant suppressed the mutant phenotype.These results indicate that PTI1 acts as a negative reg-ulator of the HR ... micrograms of the input were used as a loadingcontrol. The bottom panel shows the level of HA-OXI1 in the anti-HA immunoprecipitates. The co-immunoprecipitation experimentswere repeated three times,...

Tài liệu Báo cáo khoa học: The tandemly repeated domains of a b-propeller phytase act synergistically to increase catalytic efﬁciency doc

... We focuson the function and relationship of the two singledomains. Additionally, the possibility that fusion of the PhyH N-terminal domain to other single-domain BPPswould improve the catalytic ... they can increase the amount of available phosphate by interacting together. Additionally,fusing PhyH-DI to a single-domain phytase appears to be an efﬁcient wayto improve the activity of the ... toincrease the catalytic efﬁciency of PhyH-DII. Further-more, the catalytic efﬁciency of PhyH was much greaterthan that of PhyH-DI and PhyH-DII combined (5.73fold) or twice the amount of PhyH-DII...

Tài liệu Báo cáo khoa học: The central role of CDE/CHR promoter elements in the regulation of cell cycle-dependent gene transcription pdf

... turns of the DNA double helix yields binding of the two or three NF-Y complexes on the same side of the DNA.Conservation of spacing is required for optimal pro-moter activity because changing the ... together with the E2F site [20,21]. The DRS ⁄ CHR in the B-Myb pro-moter deviates most from other CHR sequences withits two-nucleotide exchange from the CHR consensus(Fig. 2). Changing the ... However, as in most other CDE ⁄ CHRpromoters, mutation of the CHR results in a smallerremaining cell cycle-regulation than alteration of the CDE [37]. Interestingly, the core of the human Tome-1promoter...

Tài liệu Báo cáo khoa học: The ubiquitin ligase Itch mediates the antiapoptotic activity of epidermal growth factor by promoting the ubiquitylation and degradation of the truncated C-terminal portion of Bid ppt

... initiation of apoptosis through the mitochondrial pathway. The key event in the mito-chondrial pathway is the release of proapoptotic fac-tors from the mitochondrial intermembrane space into the cytosol, ... release of cytochrome c and second mitochondria-derivedactivator of caspase (Smac ⁄ DIABLO) allows for the formation of the apoptosome, a complex that enables the activation of caspases within the ... of its target, endophilin, augments follow-ing the treatment of cells with EGF [4]. We have sinceshown that this effect is speciﬁcally due to the activa-tion by the EGF receptor of a signalling...

Tài liệu Báo cáo khoa học: The plasminogen activator inhibitor 2 transcript is destabilized via a multi-component 3¢ UTR localized adenylate and uridylate-rich instability element in an analogous manner to cytokines and oncogenes pdf

... within the context of the full-length PAI-2 3¢ UTR, and the inﬂuence of these mutations on the mRNA decay characteristics wasdetermined. As shown in Fig. 5, the estimated half-lives of these ... diagrammatic representation of the PAI-2 3¢ UTR showing the location and sequence of the AU-rich regions of interest within the ‘extended ARE’, and the sequences of the various ‘extended ARE’mutants ... the experiment (based on the composite curve of three separate experiments presented in Fig. 1), demon-strating the high stability of this transcript. The half -life of the b-globinPAI)2transcript...

Tài liệu Báo cáo khoa học: The splicing factor ASF/SF2 is associated with TIA-1-related/ TIA-1-containing ribonucleoproteic complexes and contributes to post-transcriptional repression of gene expression doc

... of the presence of ribosomal proteins in the TIAR-TAP pellet is difﬁcultto address because ribosomal proteins are commoncontaminants of TAP assays [43,44]. On the otherhand, the presence of ... tethering of TTP to the same reportermRNA. These results indicate that ASF ⁄ SF2 is apotent repressing factor in the absence of competingfactors. It is worth noting that the deletion of the ... thoseobtained in the present study might reﬂect the impor-tance of the nuclear origin of the target mRNA for the functional outcome of ASF ⁄ SF2 recruitment tomRNA 3¢ UTR.In conclusion, the present...

Tài liệu Báo cáo khoa học: The two Caenorhabditis elegans metallothioneins (CeMT-1 and CeMT-2) discriminate between essential zinc and toxic cadmium docx

... well as a very small amount of Cd6Zn1-CeMT-1 me-talloforms, were present as minor species (Fig. 1B). The contribution of these over-metallated species is also apparent in the stoichiometry ... Cd6-CeMT-2 and other MTs (Table 2), evenwhen the effect of the over-metallated species is takeninto account. To date, the overall stability of cadmiumbinding to CeMT-1 is the lowest value ... to the total EXAFSspectra much smaller than the Cd–S contribution.Nevertheless, this conﬁrms that the cadmium coordina-tion environments of the CeMT-1 KO and doubleKO differ from those of the...

Tài liệu Báo cáo khoa học: The multicopper oxidase from the archaeon Pyrobaculum aerophilum shows nitrous oxide reductase activity docx

... reﬁnement, the ﬁnal model presented 89.1% of the residues in the mostfavored regions of the Ramachandran plot, 10.9% in the additional allowed regions, and no residues in the gener-ously allowed ... described astheir the sole oxidant [1,2,9,40,51]. The main electrontransfer steps in the reaction mechanism of MCOs are:(a) the reduction of the T1 site by the substrates; (b) the electron ... have focused our attention on the study of prokaryotic MCOs, the CotA laccase from Bacillus subtilis and the metallo-oxidase McoA from Aquifex aeolicus, because of their potential for biotech-nological...

Tài liệu Báo cáo khoa học: The alr2505 (osiS) gene from Anabaena sp. strain PCC7120 encodes a cysteine desulfurase induced by oxidative stress docx

... the nifB, nifS and nifUgenes, in view of their similarity with the correspond-ing genes from other diazotrophs, may be involved in the maturation of nitrogenase [28].Group 1 all1 457nifS all1 516fdxN all1 517nifB all1 456nifUalr2505asr2504alr2495alr2505Group ... the NifS proteinsthemselves and other subsets of cysteine desulfurases thatare not restricted to diaztrophic organisms, namely the ISC and NFS proteins. All the members of this grouphave the ... Expression of the rnpB gene wasused as the control assay. All the primers used in the experiment were initially checked in PCR reactions with Anabaena genomic DNA as the template, at the same...

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