Tài liệu Báo cáo khóa học: Numerical calculations of the pH of maximal protein stability pptx

... free energy of folding was modeled numerically as a function of pH as well as the net charge of the protein. The optimum pH was determined in the numerical calculations as the pH of the minimum ... a single number for the optimum pH. If given protein is reported to be stable in a range of pHs, then the optimum pH is taken to be the middle of the...

Ngày tải lên: 19/02/2014, 12:20

... confirmed the active role of miR-23a in the growth promotion of malignant cells. The fundamental function of miRNAs is to regulate their targets by direct cleavage of mRNA or by inhibi- tion of protein ... with the first potential binding site of the IL6R mRNA 3¢ UTR. (A) The three EGFP reporter vectors bear- ing mutations of the miR-23a seed region binding site ar...

Ngày tải lên: 18/02/2014, 04:20

... AD research to increase the degree of phosphorylation of various proteins, such as tau. One of the hallmarks of AD and tauopathies is the appearance of highly phos- phorylated tau isoforms in paired ... blocks the dephosphorylation of pro- teins that are substrates for several protein kinases [8,9]. Recent advances in the analysis of the molecular interac- tions o...

Ngày tải lên: 18/02/2014, 14:20

... cleavage both in the presence and absence of Pi at pH 6.5 and pH 8.0. At pH 8.0 the digestion in the presence of cofilin was equally fast with and without Pi, while at pH 6.5 the acceleration of the cleavage ... dependence of the Pi inhibition of cofilin binding may contribute to the pH regulation of the actin depolymerizing activity of cofilin, which is i...

Ngày tải lên: 19/02/2014, 07:20

... may enter the citric acid cycle completing the catabolism of the pyrrolidine moiety of nicotine. Expression of the genes of these enzymes was dependent on the presence of nicotine in the growth ... expression of these genes depends on the presence of nicotine in the growth medium and we have determined the enzyme activities of the proteins. Our results demo...

Ngày tải lên: 19/02/2014, 07:20

... disappearance of the intermediate form of the Rieske protein. At the same time, the levels of subunits 7, 8 and 9 significantly decreased in this mutant strain. However, the amounts of core protein ... core protein 2 were relatively unaffected. Therefore, the absence of subunit 6 appeared to alter the rates of processing of two of the redox subunits and cau...

Ngày tải lên: 19/02/2014, 12:20

... on the stability of the B1 domain of Streptococcal protein G (GB1). One aspect of GB1 stability that we have addressed is the tolerance for mutations in the core of the protein. These studies provide a ... permit the rapid analysis of libraries of protein variants. Phage- display has proved to be a powerful tool for analyzing protein stability due to the...

Ngày tải lên: 19/02/2014, 12:20

... encodes the first two structural motifs, and hence the N-ter- minal domain of the protein, just as in the genes for cS and cA–F. However, the second half of the gene has the structure of a b-crystallin ... with the familiar pattern of expression of the principally embryonic cA–F genes in the rodent lens [41,47]. The absence of expression in outer layers of...

Ngày tải lên: 19/02/2014, 17:20

... also the substrate of PHD-2 and PHD-3. Thus, PHD-2, PHD-3 and P4h expression appear to be subject to a common control by oxygen, whilst the expression of PHD-1 is not. The physiological meaning of ... the question arises concerning the mechanisms underlying the orchestrated expression of these enzymes by the oxygen tension. The findings that the effect of hypoxia on...

Ngày tải lên: 20/02/2014, 02:21

... or maturation of the protein. An increase was also observed in the rate of degradation of the remaining mutant protein (20% left of the protein) vs. the wild type (40% left of the protein) at 3 h of chase (Fig. ... activation of Ga 16 and Ga q subunits. Therefore the lack of change in the rate of degradation cannot be due to lack of receptor-activa- ti...

Ngày tải lên: 20/02/2014, 03:20