Tài liệu Báo cáo khóa học: Numerical calculations of the pH of maximal protein stability pptx

... free energy of folding wasmodeled numerically as a function of pH as well as the netcharge of the protein. The optimum pH was determined in the numerical calculations as the pH of the minimum ... asingle number for the optimum pH. If given protein isreported to be stable in a range of pHs, then the optimum pH is taken to be the middle of the pH range. The optimum pH in the numerical calculation ... indetermining the optimum pH. The points below the diagonal show proteins with pH optimum lower than the pH of maximum of the free energy of unfolded state. The points offset from the diagonal manifest the...

Tài liệu Báo cáo khoa học: MicroRNA-23a promotes the growth of gastric adenocarcinoma cell line MGC803 and downregulates interleukin-6 receptor pdf

... conﬁrmed the active role of miR-23a in the growth promotion of malignant cells. The fundamental function of miRNAs is to regulatetheir targets by direct cleavage of mRNA or by inhibi-tion of protein ... with the ﬁrst potential binding site of the IL6R mRNA 3¢ UTR. (A) The three EGFP reporter vectors bear-ing mutations of the miR-23a seed region binding site are shown. The arrows indicate the ... with the 3¢ UTR of target genes. Further-more, the 2–8 nucleotides of miRNA, known as the ‘seed region’, have been suggested to be the mostimportant region for target recognition [16]. Therefore,we...

Tài liệu Báo cáo khoa học: Marine toxins and the cytoskeleton: okadaic acid and dinophysistoxins pptx

... ADresearch to increase the degree of phosphorylation of various proteins, such as tau. One of the hallmarks of AD and tauopathies is the appearance of highly phos-phorylated tau isoforms in paired ... blocks the dephosphorylation of pro-teins that are substrates for several protein kinases [8,9].Recent advances in the analysis of the molecular interac-tions of OA with Ser ⁄ Thr protein phosphatases ... classes of Ser ⁄ Thr protein phosphatases, termed PP1, PP2A,PP2B (calcineurin) and PP2C, have been identiﬁed. The number of physiological processes in which the Ser ⁄ Thr protein phosphatases...

Tài liệu Báo cáo khoa học: Inorganic phosphate regulates the binding of coﬁlin to actin ﬁlaments pdf

... cleavageboth in the presence and absence of Pi at pH 6.5and pH 8.0. At pH 8.0 the digestion in the presence of coﬁlin was equally fast with and without Pi, whileat pH 6.5 the acceleration of the cleavage ... dependence of the Pi inhibition of coﬁlin binding may contribute to the pH regulation of the actin depolymerizing activity of coﬁlin, which is important in view of the localized pH ﬂuctuations in the ... in the cleft of actin protomers locatedin the vicinity of the barbed end of the ﬁlament, pro-ducing an ATP or ADP–Pi cap at this end. Because of the presence of this cap at the barbed end the...

Tài liệu Báo cáo khoa học: Final steps in the catabolism of nicotine Deamination versus demethylation of c-N-methylaminobutyrate doc

... may enter the citric acidcycle completing the catabolism of the pyrrolidine moiety of nicotine.Expression of the genes of these enzymes was dependent on the presence of nicotine in the growth ... expression of these genesdepends on the presence of nicotine in the growthmedium and we have determined the enzyme activities of the proteins. Our results demonstrate the presence of two pathways of ... in NADPH and formaldehydemay be assimilated by the Embden–Meyerhof fructose-bisphosphate aldose ⁄ transaldolase variant of the ribu-lose monophosphate cycle [14,15]. The amino group of 4-aminobutyrate,...

Tài liệu Báo cáo khóa học: Further insights into the assembly of the yeast cytochrome bc1 complex based on analysis of single and double deletion mutants lacking supernumerary subunits and cytochrome b pdf

... disappearance of the intermediate form of the Rieske protein. At the same time, the levels of subunits 7, 8 and 9 signiﬁcantly decreased in this mutantstrain. However, the amounts of core protein ... core protein 2 were relatively unaffected. Therefore, the absence of subunit 6 appeared to alter the rates of processing of two of the redox subunits and caused minor changes in amounts of the ... Robinson, B.H. (2001) The N-terminus of the Qcr7 protein of the cytochrome bc1complex inS. cerevisiae may be involved in facilitating stability of the sub-complex with the Qcr8 protein and cytochrome...

Tài liệu Báo cáo khoa học: Phage-display as a tool for quantifying protein stability determinants pptx

... on the stability of the B1 domain of Streptococcal protein G (GB1). One aspect of GB1 stability that we have addressed is the tolerance formutations in the core of the protein. These studies providea ... permit the rapid analysis of libraries of protein variants. Phage-display has proved to be a powerful tool for analyzing protein stability due to the large library size and the robustness of the phage ... simultaneous characterization of a relatively large number of mutants. In addition, the high in vitro stability of the phage particle [6] permits the use of a wide range of selection conditions. For...

Tài liệu Báo cáo khoa học: cN-crystallin and the evolution of the bc-crystallin superfamily in vertebrates pdf

... encodes the ﬁrst two structural motifs, and hence the N-ter-minal domain of the protein, just as in the genes forcS and cA–F. However, the second half of the genehas the structure of a b-crystallin ... with the familiar pattern of expression of the principally embryonic cA–F genes in the rodentlens [41,47]. The absence of expression in outer layers of the lens contrasts with the expression of ... the b and c crystallins together account for the majority of the soluble proteins (the other majorfamily being the a-crystallins, members of the smallheat-shock protein superfamily [10,11])....

Tài liệu Báo cáo khoa học: Oxygen tension regulates the expression of a group of procollagen hydroxylases docx

... also the substrate of PHD-2 and PHD-3. Thus, PHD-2, PHD-3 andP4h expression appear to be subject to a common control byoxygen, whilst the expression of PHD-1 is not. The physiological meaning of ... the question arisesconcerning the mechanisms underlying the orchestratedexpression of these enzymes by the oxygen tension. The ﬁndings that the effect of hypoxia on the geneexpression of the ... diﬀerential regulation of PHD-gene expression remains to be clariﬁed.In spite of the similar regulation of PHD-2, PHD-3 andP4h expression by oxygen, not only the protein target butalso the intracellular...

Tài liệu Báo cáo khoa học: Proteasome involvement in the degradation of the Gq family of Ga subunits pptx

... ormaturation of the protein. An increase was alsoobserved in the rate of degradation of the remainingmutant protein (20% left of the protein) vs. the wildtype (40% left of the protein) at 3 h of chase(Fig. ... activation of Ga16and Gaqsubunits. Therefore the lack of change in the rate of degradation cannot be due to lack of receptor-activa-tion of the alpha subunits.Given that the activation of G proteins ... Shows the autoradiographies of the two mutant G proteinscompared with the wild-type protein at 0 and 3 h. (B) The relativeamounts of the [35S]Gaqsubunits were determined using a phos-phoimager...

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