Tài liệu Báo cáo khóa học: The effect of mutations surrounding and within the active site on the catalytic activity of ricin A chain pptx

... activation and plasma membrane localization of the fusion construct are dependent on phosphatidylinositol 3-kinase signal- ing, and that ITK–SYK phosphorylates the adapter proteins SLP-76 and ... capacity, membrane localization and phosphorylation of key residues? Comparison between the activation of ITK–SYK and BTK–SYK To determine its activation capacity, we construc...

Ngày tải lên: 14/02/2014, 18:20

... that differ substantially in their biochemical properties and intracellular localization. DAP-kinases (DAPk), Ca 2+ ⁄ calmodulin-regulated and Ser ⁄Thr kinases, activate signaling pathways that lead ... (mi)RNAs is still expanding, but only few have been functionally annotated. Agami and colleagues developed a library of vectors expressing the majority of cloned human miRNAs, a...

Ngày tải lên: 19/02/2014, 02:20

... with an octasaccharide of compo- sition Hex 5 HexNAc 3 . The approximate relative proportions of partially methylated alditol acetates (PMAAs) from methylation analysis (Table 1) are in agreement ... function. One of the early demonstrations of the role of carbo- hydrates in recognition was binding of the influenza virus to red blood cells via sialic acid [1], and later b...

Ngày tải lên: 19/02/2014, 12:20

... of these sequences are derived from the Mcp [11], and the Fab-7 [12] regions. Both contain a boundary and at least one PRE, and are able to medi- ate long-distance regulatory interactions via the ... abdominal segments A6 and A7 . Although the iab-5 cis-regulatory region is also in an active conformation in A6 , only iab-6 is presumed to contact the Abd-B promoter r...

Ngày tải lên: 20/02/2014, 01:20

... washed once with NaCl ⁄ P i , and then incubated in medium with 1 · 10 )8 m PA and various con- centrations of LF. The viability of RAW 264.7 cells was assessed after 4 h and 24 h, and that of ... inducing vascular leakage that leads to shock and multiorgan failure [3–6]. The role of LeTx in anthrax pathogenesis is complex, however, and probably involves the impairme...

Ngày tải lên: 16/02/2014, 09:20

... absence (one-way analysis of variance and Tukey multiple comparison test). One-way analysis of variance also showed that all values of k, with the exception of that for a 1 -PI at the lowest concentration, ... combinations of concentrations. In the case of the sulfated polysaccharides, the effector molecules are constituents of the same sample, and their effect...

Ngày tải lên: 16/02/2014, 14:20

... N-terminal hexahistidine tag was obtained by PCR using the pET2 1a ⁄ PNT- H6 plasmid [30] as the template. The forward primer (5¢-TACCGTTAACATCGATATGCATCATCATC ATCATCATGC-3¢) was designed to insert a ... as regulation and signalling in eukaryotes, prokaryotes and viruses. The func- tion of many IDPs relies upon interactions with partner proteins, often accompanied by conforma...

Ngày tải lên: 18/02/2014, 04:20

... they are hydrophobic and negatively charged. A comparison of rhodamine uptake, in the presence and the absence of uncouplers, allows the estimation of the effect of intracellular binding on the ... centrifugation through an oil cushion and dissolved prior to determination of the amount of dyes associated with them. The advantages of such an assay are th...

Ngày tải lên: 18/02/2014, 13:20

... N1C-13, LA1, LA2 (Mốc LA1, LA2 là 02 điểm Laze định hướng). Từ đó kiểm tra toạ độ, độ cao vị trí tim đường hầm tại gương đào c a mũi số 1. Tại mũi thi công số 2, kiểm tra độ ổn định c a 03 mốc ... theo thiết kế cả về mặt bằng và độ cao. Bài báo này trình bày phương pháp kiểm tra, từ đó đ a ra nhận xét về khả năng thông hướng c a đường hầm và đ a ra các kiến nghị kịp thời giúp chủ đ...

Ngày tải lên: 18/02/2014, 13:20

... activation of the Ras-signaling pathway would trigger the methylation aberrations and histone modifications that are a hallmark of cancer: regional DNA hypermethylation and global hypomethylation. An attractive ... configuration. DNA demethylation is also tightly associated with chromatin structure; histone acetylation of H3 and H4 histone tails is a hallmark of active c...

Ngày tải lên: 18/02/2014, 14:20