Tài liệu Báo cáo khoa học: Identification of membrane-bound serine proteinase matriptase as processing enzyme of insulin-like growth factor binding protein-related protein-1 (IGFBP-rP1/angiomodulin/mac25) doc

... of DNAs and RNAs. Fig. S3. Binding affinity of EWS and RBD to Htelo. Fig. S4. In vitro arginine methylation of RGG3 by PRMT3. Fig. S5. Identification of significant residues at RGG3 for rHtelo binding ... the G-quadruplex DNA binding, but also to G-quadruplex RNA binding, we assayed the binding of RGG3 with a human telomere RNA (rHtelo) in the presence of K + , which exists...

Ngày tải lên: 15/02/2014, 01:20

... with other known sequences using the fasta [25] and blast [26] suite of pro- grams. Homology analysis of the amino acid sequences was performed using matgat software v2.02 [75], and multiple sequence ... IL-25 (also known as IL-17E), which are important in the generation of the correct class of antibodies by B-cells, and for the elimination of extracellular pathogens, such as he...

Ngày tải lên: 16/02/2014, 09:20

... endonucleases, T4 DNA ligase, E. coli DNA polymerase I Klenow fragment, and Taq DNA poly- merase were purchased from Fermentas (Hanover, MD, USA). Preparation of plasmids, and purification of PCR products ... organic phase by three-fold extraction with 50% NaCl ⁄ chloroform (1 : 1 v ⁄ v). A new volume of chloroform was used for each of the three extractions. Finally, the organic phase...

Ngày tải lên: 18/02/2014, 14:20

... coexpressed in yeast. As compared to the SmSmad1B–SmSmad4 interaction observed in the yeast two-hybrid assay, the inclusion of SmTbRI or SmTbRIQD resulted in decreased growth of cotransformed yeast on ... protein interactions Yeast two-hybrid and three-hybrid assays The yeast strain Y190, a HIS3 ⁄ LacZ reporter strain, was utilized in the yeast two-hybrid assays. Preparation of yeas...

Ngày tải lên: 18/02/2014, 16:20

... transcription was con- ducted with a random primer. Each reaction mixture was composed of 2 lL of Moloney murine leukemia virus reverse transcriptase buffer; 0.5 lL of BSA (10 mgÆmL )1 ), 1 lL of total ... detection of RpCAtr mRNA in both the epidermal cytoplasm of the branchial filaments and in the cytoplasm of the trophosomal bacteriocytes. We could only detect RpCAbr mRNA in t...

Ngày tải lên: 18/02/2014, 16:20

... two constitutive isoforms of HSP70 are synthesized in the gills and mantle of C. gigas [26]. The expression level of the constitutive forms increases after thermal stress, whereas the inducible ... also known as ribo- somal protein L10, is a transcription cofactor that inhibits activation of AP-1 transcription factors. QM protein is implicated in the conversion of a broad vari- et...

Ngày tải lên: 18/02/2014, 16:20

... surfaces, basement membranes and bone matrix, and are involved in bone formation through regulation of cell differentiation factors such as bone morphogenetic proteins and fibroblast growth factors ... After wash- ing the beads with 10 mm NaCl ⁄ P i (pH 7.4), complete binding of the antibody was confirmed by the detection of no bands in the supernatant SDS–PAGE. Subsequently, 100 lL...

Ngày tải lên: 18/02/2014, 17:20

... for soyasapogenol A is not as simple as that for soyasapogenol B, and the presence of additional hydroxylases must be considered. Fortunately, the aglycone of the major soyasaponins is soyasapogenol ... the hydroxylation of campestanol by two inde- pendent P450 (6-hydroxylase and 22-hydroxylase) and joining at the formation of castasterone by the same enzymes was proposed in brass...

Ngày tải lên: 19/02/2014, 07:20

... analysis of the contribution of the STAT2 DNA binding domain to IFN responses on a global basis, and shows that STAT2 is required for the IFN-inducible activation of the full spectrum of GAS target ... Characterization of the induction levels of a subset of ISGF3-independent STAT2-dependent ISGs identified by BSTVQ. The differential expression of four of the ISGs examined i...

Ngày tải lên: 19/02/2014, 07:20

... compilation ª 2006 FEBS 627 Identification of membrane-bound serine proteinase matriptase as processing enzyme of insulin-like growth factor binding protein-related protein-1 (IGFBP-rP1/angiomodulin/mac25) Sanjida ... membrane- bound serine proteinase matriptase as a processing enzyme of IGFBP-rP1 ⁄ AGM. Matriptase was initially Fig. 8. Cleav...

Ngày tải lên: 19/02/2014, 07:20