Tài liệu Báo cáo khoa học: Diversity and junction residues as hotspots of binding energy in an antibody neutralizing the dengue virus doc

... within and outside the CDRs [12–16]. The CDR3 loops of V H and V L contain the residues of diversity and junction, encoded by the D segment, and the V H -D, D-J H , and V L -J L junctions [17]. They ... 273 (2006) 34–46 ª 2005 The Authors Journal compilation ª 2005 FEBS Diversity and junction residues as hotspots of binding energy in an a...

Ngày tải lên: 19/02/2014, 07:20

... syn- thesis in vivo. To investigate the reaction mechanism of H. thermo- philus POR, we measured the EPR spectra of the enzyme in the presence of various combinations of sub- strates. Intra- and intermolecular ... coupled assay and also with respect to obtaining a deeper under- standing of the metabolism of H. thermophilus. Indeed, this would enable the kinetic...

Ngày tải lên: 16/02/2014, 09:20

... and arginase share the control of the fluxes through the pathways involving arginine as a precurser of nitric oxide and polyamines ODE ss, sim, MCA Own in PERL NG Refers to single time point of ... succeeded in describing and interpreting the effects of ethanol stress. In particular, the ratio between the kinetic constants associated with ethanol production a...

Ngày tải lên: 14/02/2014, 14:20

... H3, meaning that it can direct recruitment of HBO1 both in advance of and in the wake of a transcribing polymerase. The other most likely candidates driving histone acetylation during transcription ... major role in assisting the binding of AP-1 via cooperative binding, and creates an accessi- ble environment that also enables Sp1 and Runx1 binding [85,86,233]...

Ngày tải lên: 14/02/2014, 18:20

... repressing the indicated targets and presumably hundreds of other as yet unidentified targets. (B) miR-34a and the miR-34a -binding site in the 3¢ UTR of genes shown in (A). miRNAs and cell signaling ... Involvement of miRNAs in various signaling cascades. Many miRNAs are under the control of various conserved signaling pathways and in turn regulate component...

Ngày tải lên: 14/02/2014, 19:20

... Epigenetically-regulated miRNAs. The numbers in the binding sites’ column represent the distance (bp) between the stop codon and binding sites of seed sequences in the miRNAs. The letters ‘c’ and ‘p’ with respect ... stem-loop-shaped RNAs are called pre-miRNAs, and the protein complex of RNase, Drosha and DGCR8 is known as the micro- processor complex. Pre...

Ngày tải lên: 14/02/2014, 19:20

... repolarization and the induction of PVCs and arrhythmias. On the other hand, blocking miR-1 function with antisense oligoribonucleotides was found to normalize the expression of Cx43 and Kir2.1, prevent ... novel insight into the molecular understanding of cardiac excitability. However, considering the inherent capacity of miR- NAs to target a broad range of proteins...

Ngày tải lên: 14/02/2014, 19:20

... interesting homologs [2–4]. In humans, angiogenin (RNase 5) is an inducer of neovascularization, and plays an important role in tumor growth [5]. Eosinophil-derived neurotoxin (RNase 2) and eosinophil ... development and use of N-acylsulfonamides and sulfonimides as antagonists of nucleic acid -binding proteins. Database Structural data for the two RNase A complexes...

Ngày tải lên: 14/02/2014, 22:20

... dramatic increase in the ratio of nucleotidase to phosphotrans- ferase activity. The affinity for the substrates inosine and IMP remains unaltered, and the effect of the enzyme activators ATP and Mg 2+ is ... transfer of phosphate can lead to phosphorylation of inosine, guanosine and a number of their analogues [2]. There- fore, in addition to being involved in...

Ngày tải lên: 15/02/2014, 01:20

... and hypoxanthine. (B) HPRT and hypoxanthine. (C) PRTFDC1 and guanine. (D) HPRT and guanine. Table 2. K m and V max values for Hx and G in the presence of 1 mM PRPP, determined using the DE-81 ... functions as a catalytic base [12]. However, a tight binding of N7 of the purine base and Asp137 was observed in HPRT in complex with ImmGP, indicating a role in...

Ngày tải lên: 15/02/2014, 01:20