Tài liệu Báo cáo khoa học: Domain deletions and substitutions in the modular protein evolution doc

... triphosphate hydrolase domain, the epider- mal growth factor (EGF) domain, the SH3 domain, the P-kinase domain and the domains involved in the blood clotting cascade [1,10]. The phenomenon of differential ... at either of the protein termini, and that their occurrence is slightly higher at one of the termini, depending on the number of domains in the protei...

Ngày tải lên: 19/02/2014, 07:20

... lower curve). The calculated kinetic parameters of the interaction between cubilin and IFs from different sources are presented in Table 1. Both the natural proteins and the recombinant product ... trans- porting proteins and several receptors which together ensure its efficient uptake [2–4]. Intrinsic factor (IF) is responsible for intestinal absorption of vitamin B 12 facilit...

Ngày tải lên: 21/02/2014, 00:20

... Tyr304 in the cytoplasmic tail of ephrin B2 confers high-affinity and bifunctional binding to both the SH2 domain of Grb4 and the PDZ domain of the PDZ-RGS3 protein Zhengding Su, Ping Xu and Feng ... RGS3 PDZ domains The DNA sequences encoding the Grb4 SH2 and the RGS3 PDZ domains were individually deduced from the amino acid sequences of murine Grb4 protein...

Ngày tải lên: 19/02/2014, 12:20

... divided in two phases according to the intensity and the duration of the stress. An initial adaptive response culminates in the temporary inhibition of protein synthesis, providing cells with the ... kinase C, and this effect was attributed to the formation of gel-like domains (instead of liquid- crystalline domains) in the membranes, making these molecules unavailab...

Ngày tải lên: 14/02/2014, 22:20

... all-alpha domain, STAT protein DNA-binding domain and SH2 domain of stat6, and the zinc-finger domain, leucine-zipper domain and fork-head domain of foxp3. In addition, for stat6 and foxp3, there ... FOX proteins, a leucine zipper domain and a C2H2 zinc finger domain, both of which are thought to help mediate DNA binding and may be involved in the induction of...

Ngày tải lên: 16/02/2014, 09:20

... Y2, Y4, Y5 and y6) that vary in their binding profiles and in their distribution in the central nervous system and periphery. Y1, Y2 and Y5 are the best characterized NPY receptors, and the majority ... FEBS 3665 (ruling out the possibility of an autocrine, paracrine or endocrine mechanism of regulation in the ob ⁄ ob model), and given that the majority of lepti...

Ngày tải lên: 18/02/2014, 04:20

... activation of the sterol regulatory element-binding protein cleavage activating protein ⁄ sterol regulatory element-binding protein pathway. Mol Endocrinol 15, 1817–1828. 13 Yang YA, Morin PJ, Han ... molecule in the liver and pancreatic b-cells [8,26]. Suppression of IRS-2 by SREBP-1c in the liver inhibits processes regulating insulin signaling, such as glycogen synthesis,...

Ngày tải lên: 18/02/2014, 13:20

... higher than for the THF:A ligand. The presence of Mg 2+ had little effect on the binding of Fpg to the THF:C ligand and slightly improved binding to the THF:A ligand, making it comparable with binding to ... Kinetic and binding parameters for the processing of 8-oxoG:C and 8-oxoG:A by Fpg and OGG1 were deter- mined under several different conditions. Polyamines, crowding a...

Ngày tải lên: 18/02/2014, 18:20

... product, and an XhoI site in the 3¢-end. The amplification product was cloned into the TA cloning vector PCR4-TOPO (Invitrogen, Carlsbad, CA) for sequencing and then subcloned into vector pCDNA II (In- vitrogen) ... and averaged, each containing 1000 data points. The absorbance changes accompanying enzyme reduction with benzylamine were monophasic, and observed rates were ob...

Ngày tải lên: 19/02/2014, 07:20

... preceded by an increase in the recording’s baseline, suggesting that toxin incorporation induced an increase in the membrane conductance. Single-channel current vs. voltage (I ⁄ V) plots of the activity ... TMA-DPH incorporated into spores of F. solani in the absence of VtA 3 (n) and in the presence of 1.5 l M VtA 3 (s )and3 lM VtA 3 (n). Insert in (B) shows the evolu...

Ngày tải lên: 19/02/2014, 07:20