Tài liệu Báo cáo khoa học: Biochemical characterization of human 3-methylglutaconyl-CoA hydratase and its role in leucine metabolism docx

... 2006 FEBS Biochemical characterization of human 3-methylglutaconyl-CoA hydratase and its role in leucine metabolism Matthias Mack 1 , Ute Schniegler-Mattox 2 , Verena Peters 3 , Georg F. Hoffmann 3 , ... 3-MG-CoA hydratase reaction of leucine catabolism at the protein and DNA levels and developed a novel assay for enzyme analysis in a diagnostic setting. Th...

Ngày tải lên: 19/02/2014, 07:20

... under aerobic and anaerobic condi- tions, in the range of 0.6 lmolÆmg )1 Æmin )1 . No evidence was obtained for redox cycling of FMN. The amino acid residues involved in the FMN binding site are ... M15-pQEidi2. Preparation of the recombinant Idi-2 protein The recombinant E. coli strain M15-pQEidi2 was grown in Luria-Bertani broth containing ampicillin (180 mgÆL )1 )and kanamy...

Ngày tải lên: 19/02/2014, 13:20

... characterization of HpSDH demonstrates its activity with k cat of 7.7 s )1 and K m of 0.148 mm toward shikimate, k cat of 7.1 s )1 and K m of 0.182 mm toward NADP, k cat of 5.2 s )1 and K m of ... com- prising two domains that are responsible for binding substrate and NADP cofactor. The detailed structural information might expedite the discovery of novel SDH inhib...

Ngày tải lên: 19/02/2014, 05:20

... proteins are a large family of RNA-binding proteins found in all eukaryotes. These proteins reduce the expression of mRNA targets by binding in 3¢ UTR regulatory elements, thus controlling translation ... cis-acting elements usually located in the 3¢ UTR. In recent years, and as a result of great efforts aiming to understand the mechanism of RNA control in animals, the fun...

Ngày tải lên: 18/02/2014, 06:20

... ligand binding, and the role of storage proteins, the cupins constitute one of the most diverse superfamilies of proteins known. They have been described from all three domains of life [1,2], and usually ... Straganz and Bernd Nidetzky Institute of Biotechnology and Biochemical Engineering, Graz University of Technology, Austria Introduction In terms of their physi...

Ngày tải lên: 18/02/2014, 06:20

... earlier, a value of 2.8 · 10 7 Æm )1 Æmin )1 was obtained for the second order rate constant k 2 ⁄ K M . Using this value of k 2 ⁄ K M and the values of k 2 and k )1 (4.1Æmin )1 and 0.9Æmin )1 respectively), ... 3¢-terminal guanosine of the ribozyme as the first-step nucleophile, whereas self-splicing utilizes an exog- enous guanosine. To further aid in our understanding of r...

Ngày tải lên: 18/02/2014, 18:20

... band appears at around 660–675 nm; this increases in inten- sity with time, indicating the formation of biliverdin. In this case, neither bands of the oxy form (at 541 and 578 nm) nor bands of ... con- verts combined heme into biliverdin IX a , retaining a-regiospecificity, and releasing CO and free iron, in the presence of oxygen and NADPH ⁄ FNR ⁄ Fd, with- out requiring...

Ngày tải lên: 19/02/2014, 05:20

... mechanism of ligand–receptor interaction has been proposed for all chemokines. Binding involves interac- tions between the chemokine ligand N-loop and the receptor N-domain, and ligand N-terminal and ... major role in determining binding affinity and not in ligand selectiv- ity [22–24]. Design and characterization of IL-8(1–66) monomer Previous studies using a ‘trapped’ m...

Ngày tải lên: 19/02/2014, 05:20

... consisting of four copies of four subunits, a, b, c and d. The a and b subunits are regu- latory and are the sites of phosphorylation and meta- bolite binding and are also regulated by the binding of ... extension to the protein kinase folds back on the kinase domain and interferes with the substrate binding sites. In the case of the titin and twitchin kinases, the a...

Ngày tải lên: 19/02/2014, 17:20

... Dashed lines indicate van der Waals contacts with interatomic distances in A ˚ . Dotted lines indicate C-HÆÆÆO interactions. Black lines indicate interac- tions in SQV complexes. Red lines indicate interactions ... different inhibitors will give insight into the mechanisms of resistance and help in the design of new inhibitors. Results APV inhibition of PR and mutants The kine...

Ngày tải lên: 18/02/2014, 04:20