Tài liệu Báo cáo khoa học: Identification of b-amyrin and sophoradiol 24-hydroxylase by expressed sequence tag mining and functional expression assay docx

... Affinity of RGG3 for binding to a G-quadru- plex DNA and RNA. Fig. S2. CD spectra of DNAs and RNAs. Fig. S3. Binding affinity of EWS and RBD to Htelo. Fig. S4. In vitro arginine methylation of RGG3 by PRMT3. Fig. ... (lanes 2 and 4) and 32 P-labeled Htelo (lanes 1 and 2) or rHtelo (lanes 3 and 4). (B) EMSA was performed with RGG3 (lanes 2 and 4) and 32 P-labeled rHte...

Ngày tải lên: 15/02/2014, 01:20

... identified by digestion with BamHI and SacI as well as by sequence analysis, and named pWlpxX and pWlpxL. pWlpxX and pWlpxL were used to transform O35ElpxX and O35ElpxL competent cells by electropora- tion, ... and structural analysis of OSs in LOSs of lpxX and lpxL mutants The OS portion of each LOS was obtained after mild acid hydrolysis and analyzed for its gly...

Ngày tải lên: 18/02/2014, 14:20

... short time-window at days 14 and ⁄ or 17. HYPK and QM protein were underexpressed at days 3, 7 and 24 and day 7, respectively. Discussion Of 322 genes identified in this study, 191 partial sequences had not ... HSPs (HSP70 and HSP23) were greatly and rapidly upregu- lated in gills but slightly less and later in mantle. One inducible and two constitutive isoforms of HSP70 ar...

Ngày tải lên: 18/02/2014, 16:20

... vitro,is expressed in the early stages of development of the inner ear (48 hpf) and is involved in the seeding and ⁄ or nucleation of the sagitta and lapillus [23]. On the other hand, omm-64 may be expressed ... found to have a molecular mass of 64 kDa, and to contain two tandem repeats and a Glu-rich region. The structure of the protein and that of its DNA are sim...

Ngày tải lên: 18/02/2014, 17:20

... Authors Journal compilation ª 2006 FEBS 959 Identification of b-amyrin and sophoradiol 24-hydroxylase by expressed sequence tag mining and functional expression assay Masaaki Shibuya 1 , Masaki Hoshino 1 , ... range of structural diversity produced by a sequence of biosynthetic reactions. Cyclization of oxidosqualene is the ini- tial origin of structur...

Ngày tải lên: 19/02/2014, 07:20

... similar levels of expression and assigns the average level of gene expression a color value. Regions in red represent highly expressed or present genes and those in blue represent unexpressed or ... number of genes in U6A and U6A-2 cells, IFN-treated U6A-VV-II cells showed the down-regulation of a substantially larger subset of genes. Identification and characterizati...

Ngày tải lên: 19/02/2014, 07:20

... discussions. This work was supported by Grants-in-Aid from the Minis- try of Education, Culture, Sports, Science and Tech- nology and from the Ministry of Welfare and Labor of Japan. References 1 Jones ... clearly showed doublet bands at approximately 75 kDa and two minor bands at 95 and 110 kDa. The conditioned media of HSC-4 and HEK293 cells also showed weak immunoreac...

Ngày tải lên: 19/02/2014, 07:20

... as required. Construction of YEF1 expression plasmid and strain YEF1 was amplified from genomic DNA of S. cerevisiae BY4 742 with PfuUltra high-fidelity DNA polymerase (Stratagene, La Jolla, CA, USA) by using PCR and was ... 40 nucleotides upstream and downstream of POS5 was amplified by PCR from plasmid pCgLEU2 by using primers pos5leu21.6f and pos5leu21.6r and was intr...

Ngày tải lên: 20/02/2014, 01:20

... view of the active site of ACE2 and tACE. Binding interactions of the inhibitor (A) MLN-4760 at the active site of ACE2 and (B) lisinopril at the active site of tACE. Hydrogen bonds to the ligand ... Mca- Fig. 3. Role of His505 and His345 in catalysis. Schematic of the proposed reaction intermediate of ACE2, showing the importance of His345 and His505. Hydrogen bonds...

Ngày tải lên: 20/02/2014, 01:20

... h of incubation, 7.5 l M of GT oligomer or control CT sequence were added to the cells. The percentage of viable cells was assayed after 72 h of incubation by determining the incorporation of ... (i.e. m/z 2201.3 and 1649.7) allowed internal sequence tags to be obtained, definitively demonstrating the nature of this species (Fig. 2B). The identity of P1 was also assayed...

Ngày tải lên: 21/02/2014, 00:20