Tài liệu Báo cáo khoa học: Mixed-type noncompetitive inhibition of anthrax lethal factor protease by aminoglycosides ppt

... (Houston, TX, USA). Protease assays The lethal factor protease was assayed according to the FRET method, first described for lethal factor protease by Cummings et al. [16]. Lethal factor protease (10 ... from the softmax pro software (Molecular Devices) and analyzed by using the software batchki (BioKin Ltd, Pullman, WA, USA). P. Kuzmic et al. Inhibition of lethal...

Ngày tải lên: 19/02/2014, 06:20

... (2003) Functions of propeptide parts in cysteine proteases. Curr Protein Pept Sci 4, 309–326. 3 Cygler M & Mort JS (1997) Proregion structure of members of the papain superfamily. Mode of inhibition of ... the N-terminus of the protease acts as an intramolecular chaperone to mediate correct folding of the protease [2]. The mature catalytic domain of the enzyme of t...

Ngày tải lên: 14/02/2014, 14:20

... achieving inhibition of tumour cell proliferation. Indeed, its inhibition results in cell death. We previously observed that inhibition of the transcription factor nuclear factor- jB, a key regulator of cell ... induction of cell death. Several therapeutic approaches have been developed to inhibit STAT3, including inhibition of its expression [14,15], inhibition of it...

Ngày tải lên: 18/02/2014, 08:20

... properties of M b through functionalization of the pigment by c hemical modification of its prosthetic heme unit [299]. Thus, studies on the NADH-driven deformylation of 2-phenyl- propionaldehyde by rMb, ... 2004 model constructed by alignment of the CYP19 sequence with the known crystal structures of bacterial P450s, E302 was postulated t o be essential to activation of th...

Ngày tải lên: 19/02/2014, 16:20

... k train (w) denote the number of occurrences of w in the training corpus, and k test (w) denote the number of occurrences of w in the test corpus. We define the empirical discount of w to be d(w) = k train (w) ... one of 110M words of NYT from 2000 and 2001 (NYT00+01), and one of 110M words of AFP from 2002, 2005, and 2006 (AFP02+05+06). In both cases, we compute ¯ d(i) and t...

Ngày tải lên: 20/02/2014, 04:20

... trees for x. By relating the sum of the scores of all possible trees to counting the number of spanning trees in a graph, it can be shown that Z x is the determinant of the Kirchoff matrix K, ... marginal probability of a particular edge k → i (i.e. y i =k), the score of any edge k  → i such that k  = k is set to 0. The determinant of the resulting modi- fied Kirchoff matrix...

Ngày tải lên: 20/02/2014, 07:20

... 2 NADPH þ CO 2 followed by a reversible limb comprising many of the reactions of the regenerative limb of Calvin cycle with the addition of transaldolase. The function of this pathway is less clearly ... some general property of the model or to some inauspicious choice of parameter values: the large number of parameters in any realistic model obviates the possibility of a...

Ngày tải lên: 20/02/2014, 23:20

... Discussion Inhibition of proteases by MA It has been previously proposed that MA may inhibit the activity of proteases of T. gondii [14] and HIV [12,31]. To ascertain the inhibition range of MA on the ... Non-specific inhibition of metalloprotease activity can readily be achieved by chelating agents that bind to metal cations required in the active site of the enzyme. The...

Ngày tải lên: 14/02/2014, 14:20

... Composition of nucleosomes. The assembly of the histone octamer on DNA is represented by this model which depicts the incorporation of two H3 ⁄ H4 dimers with an inner core of  60 bp of DNA, followed by ... deacetyla- tion, and transient recruitment of remodellers and transcription factors. A cycle of transcription commences with the recruit- ment of transcription factors...

Ngày tải lên: 14/02/2014, 18:20

... types of organisms, including the vast major- ity of eukaryotes and many prokaryotes. It is well established that Sco pro- teins in eukaryotes are involved in the assembly of the Cu A cofactor of mitochondrial ... basis of genetic data [17], that the molecular basis for the mitochondrial signal might be generated by Sco2-dependent modulation of the redox state of the cysteines...

Ngày tải lên: 14/02/2014, 18:20