... [40], a truncated form of human CPR [41], and ratBVR [42].Heme binding and preparation of heme–GmHO-1 To determine the stoichiometry of heme binding to GmHO-1, 24 lL of a 200 lm protein solution ... heme degradation rate of GmHO-1, indi-cated in Table 4, is comparable to that of SynHO-1 inthe presence of NADPH, FNR, and Fd, and also to that of rHO-1 in the presence of NADPH and CPR.Here, ... sequences for ligation to the 5¢-ends of Oligo IV, VI, VIII and X. The 3¢-end of Oligo X had asequence designed to ligate to the HindIII site. To completethe GmHO-1 expression vector pMWGmHO-1, double-stranded...