Tài liệu Báo cáo khoa học: Thermodynamic characterization of interleukin-8 monomer binding to CXCR1 receptor N-terminal domain ppt

... trapped L25NMe monomer to the CXCR1 N -domain peptide. We used ITC to determine the enthalpy (DH), entropy (DS), and the free energy (DG) of binding of monomeric IL-8 to the receptor CXCR1 N -domain. The binding ... elicit their function by binding receptors of the G-protein-cou- pled receptor class, and the N-terminal domain (N -domain) of the receptor i...

Ngày tải lên: 19/02/2014, 05:20

... 5459 Binding of APUM to NRE To test the predictions regarding the RNA -binding specificities of the putative APUM proteins, we investi- gated the capacity of APUM to bind to the NRE sequence of hunchback ... NRE(A ) B + ), Table 2. The 12 Arabidopsis PUF domains most similar to the Dro- sophila PUF domain. Gene ID Similarity to Drosophila PUF domain (%) Identity to...

Ngày tải lên: 18/02/2014, 06:20

... that, according to prior studies of nonheme Fe 2+ centers [29–31], is assigned to the mixing of the 4p orbital with the 3d orbital of the metal cofactor. Two important pieces of information can ... (2008) Characterization of metal binding in the active sites of acireductone dioxygenase isoforms from Klebsiella ATCC 8724. Biochemistry 47, 2428–2438. 38 Harding MM (2006) Sma...

Ngày tải lên: 18/02/2014, 06:20

... allowed to proceed to completion and then an excess of unlabeled 5¢ exon mimic was added (Fig. 7A). Addition of a large excess of unlabeled 5¢ exon mimic is expected to prevent rebinding of any ... cooled to 44 °C, 2.5 lL of a stock of 0.5 nm radiolabeled 5¢ exon mimic in H10Mg buffer at 44 °C was added. The mixture was incubated at 44 °C for at least 90 min. To maintain...

Ngày tải lên: 18/02/2014, 18:20

... [40], a truncated form of human CPR [41], and rat BVR [42]. Heme binding and preparation of heme–GmHO-1 To determine the stoichiometry of heme binding to GmHO-1, 24 lL of a 200 lm protein solution ... heme degradation rate of GmHO-1, indi- cated in Table 4, is comparable to that of SynHO-1 in the presence of NADPH, FNR, and Fd, and also to that of rHO-1 in the pres...

Ngày tải lên: 19/02/2014, 05:20

... well as crotonyl-CoA, 3-hydroxybutyryl-CoA and 3-methylcrotonyl-CoA were used for the kinetic characterization of AUH. The data are summarized in Table 2. Overexpression of a mutant form of AUH and its ... (AUH). According to this scheme (E)-3-MG-5-CoA (right) bound to Gct isomerizes to give (E)-3-MG-1-CoA. (B) The production of (Z)-3-MG-5-CoA by Gct is probably due to the p...

Ngày tải lên: 19/02/2014, 07:20

... of the enzyme for that cofactor and the apparent absence of a redox process as part of the catalytic cycle. The substrate binding site of the type II enzyme remains veiled. However, a patch of ... with a subunit mass of 39 kDa was purified to apparent homogeneity by column chromatog- raphy. The protein was shown to catalyse the conversion of dimethylallyl diphosphate into i...

Ngày tải lên: 19/02/2014, 13:20

... enthalpy of binding (DH°) a nd entropy of binding (DS°) a ssociated with the interaction of CuTCPP, H 2 TPPS, CuTMPyP and H 2 TMPyP were obtained by means of van’t Hoff plots (Fig. 6) according to the ... a-helix and 8% d istorted a-helix which a dds up to 13% of a-helical structures. Regular b-sheet structure was 23% and distorted b-sheet was 13%, yielding a total of 36%...

Ngày tải lên: 19/02/2014, 16:20

... conforma- tional change occurring on binding of the peptide and could indicate that the binding of PhK5 to Ca 2+ ⁄ CaM is similar to that observed for Ca 2+ ⁄ CaM binding to isolated peptides from other ... consisting of four copies of four subunits, a, b, c and d. The a and b subunits are regu- latory and are the sites of phosphorylation and meta- bolite binding and a...

Ngày tải lên: 19/02/2014, 17:20

... help- ing to facilitate the release of the cargo within the nucleus [10,11]. The removal of the autoinhibitory domain to create a truncated importin-a avoids possi- ble competition for the binding ... domain. The importin-b binding domain is known to have an autoinhibitory function, whereby an internal NLS-like sequence competes for the importin-a binding site, reducing...

Ngày tải lên: 14/02/2014, 19:20