Tài liệu Báo cáo khoa học: Quantitative analysis of the experimental O–J–I–P chlorophyll ﬂuorescence induction kinetics Apparent activation energy and origin of each kinetic step Steve Boisvert, David Joly and Robert Carpentier doc

... Quantitative analysis of the experimental O–J–I–P chlorophyll ﬂuorescence induction kinetics Apparent activation energy and origin of each kinetic step Steve Boisvert, David Joly and Robert Carpentier Groupe ... membranes can be modeled by the superposition of the exponential rise to analyzequantitatively the contribution of each phase. The amplitude and rate constant of each of the threephases can be ... related to the reduction of the PQ pool.ResultsAs reported in the literature, the I step of the O–J–I–P ﬂuorescence transient cannot be clearly distinguishedby visual analysis of the FI traces...

Tài liệu Báo cáo khoa học: Functional analysis of the basic helix-loop-helix transcription factor DEC1 in circadian regulation ppt

... DEC1 with BMAL1 and the bin ding to the E-box.Substitution of Arg65 for Ala abolished the interaction of DEC1 with BMAL1 and the binding of DEC1 to the E-box.However, substitution of His57 for ... increase in the mutantmice, the disruption of DEC1 might be compensated byDEC2. Further investigation is required to clarify the functions of DEC1 and DEC2. The suppressive activities of DEC1 and DEC2 ... levels of expression. The transcriptional activity of FLAG-BMAL1, and the suppressive activity of FLAG-DEC1, were s imilar to those of intact BMAL1 and DEC1, respectively (Fig. 1A,C). These ﬁndings...

Tài liệu Báo cáo khoa học: Fluorescence analysis of the Hansenula polymorpha peroxisomal targeting signal-1 receptor, Pex5p pdf

... calculation of the fraction (fa) and the effectivequenching constant (KQ) for the most accessible class of Trp chromophores [16]. The pH dependence of the Trp ﬂuorescence was studiedin the pH ... spectroscopy. The intensity of Trp ﬂuorescence of puriﬁed HpPex5p increased by 25%upon shifting the pH from pH 6.0 to pH 7.2. Together with the results of ﬂuorescence quenching by acrylamide, thesedata ... 348.8 nm. Analysis of the ﬂuorescence intensity decay and anisotropy decay wasperformed with a model of discrete exponentials using the TRFA Data Processing Package of the Scientiﬁc SoftwareTechnologies...

Tài liệu Báo cáo khóa học: Quantitative analysis, using MALDI-TOF mass spectrometry, of the N-terminal hydrolysis and cyclization reactions of the activation 2 process of onconase pdf

... (Met1)-ONC(M23L) by the addition of AAP, as described in the Materials and methods. Completion of the hydrolysis wasconﬁrmed by the disappearance of the (Met1)-ONC(M23L) signal in the MALDI-TOF mass spectra ... analyzed by MALDI-TOF MS. The addition of the protease is considered the zero time point. The relative intensities of the peaks from the mass spectrawere used to calculate the fraction of Pyr-ONC (M23L), ... using other methods for the production and puriﬁcation of the enzyme [13]. The molecular mass of each puriﬁed product, as measured by MALDI-TOF MS, was11 947.87±2 for (Met1)-ONC (M23L) and 11...

Tài liệu Báo cáo khoa học: Proteomic analysis of dopamine and a-synuclein interplay in a cellular model of Parkinson’s disease pathogenesis docx

... gels of each experimental condition were built and then compared; spots were quanti-ﬁed on the basis of their relative volume (spot volume nor-malized to the sum of the volumes of all the representativespots) ... apoptosis and induces the synthesis of antioxidants [22].Alterations in mitochondrial proteins, on the otherhand, are speciﬁcally linked to one of the major patho-genetic mechanisms of PD [5]. ... stress at the IKK complex: regulation of the IKK complex byHSP70 and HSP90. Immunol Lett 117, 9–15.18 Brand IA & Heinickel A (1991) Key enzymes of carbo-hydrate metabolism as targets of the...

Tài liệu Báo cáo khoa học: Kinetic analysis of effector modulation of butyrylcholinesterase-catalysed hydrolysis of acetanilides and homologous esters pdf

... the titrant. The active siteconcentration of wild-type BuChE was 0.16 ± 0.01 lm, and that of the D70G mutant was 0.196 ± 0.02 lm.Effects of ligands on steady-state kinetics The effects of the ... free of albumin and any other AAAs. It wasfound that there was no fundamental difference in the mechanisms of inhibition and activation for either the AAA or esterase activities by these ligands. ... ester).Results and DiscussionAction of reversible effectors on AAA activity of BuChE The investigation of the effects of the ligands(tyramine, serotonin and benzalkonium) on the AAA and esterase...

Tài liệu Báo cáo khoa học: Thermodynamic analysis of porphyrin binding to Momordica charantia (bitter gourd) lectin pptx

... of MCL and the effect of ligand binding on it, the far-UV CD spectra of MCL in the native state as well asin the presence of CuTCPP and CuTMPyP were analysedby the CDSSTRprogram using the routines ... investigated the interaction of several water-soluble porphyrins with MCL. The thermodynamic forces governing the interaction of some of the porphyrins have been delineated from an analysis of the temperature ... spectra and difference spectra in the Soret band region (not shown). Analysis of association constants and thermodynamicparametersA binding curve depicting progress of the titration of CuTCPP...

Tài liệu Báo cáo khoa học: Subproteomics analysis of Ca2+-binding proteins demonstrates decreased calsequestrin expression in dystrophic mouse skeletal muscle pdf

... ÔStains-AllÕ analysis of Fig. 3 and the 1D i mmunoblotting of Fig. 4.As the full-length Dp427 isoform of dystrophin does notenter the second dimension of conventional 2D gels, the expression level of ... CSQ), and calmodulin (CAM), respectively. The pH values of the ﬁ rst dimensiongel system and molecular mass standards (in kDa) of the seconddimension are indicated at the top and on the left ... withantibodies to the Dp427 isoform of dystroph in (A), the a-subunit of the d ystroglycan complex ( a-DG; B), the N a+/K+ATPase (N KA, C), the fast SERCA1 i soform of the sarcoplasmic r...

Tài liệu Báo cáo khoa học: Quantitative estimation of channeling from early glycolytic intermediates to CO2 in intact Escherichia coli pdf

... made in the pathway dissociate from PGI and equilibrate with the pool of Fru6P in the aqueous cytoplasm, then therewould be no channeling. When there is no channeling, the result of the competition ... Fru6P, but the signa-ture of that channeling would be lost because Fru6Pwas apparently not channeled. The data in Table 3 show the method for and the results of calculating the fraction of the total ... FEBS the product of the ﬁrst enzyme to have an advantagein competition for the active site of the second enzymecompared with the same molecular species within the aqueous cytoplasm of the cell....

Tài liệu Báo cáo khoa học: Mutational analysis of plasminogen activator inhibitor-1 Interactions of a-helix F and its neighbouring structural elements regulates the activity and the rate of latency transition pdf

... bond, linkage of the activesite Ser of the protease to the carboxyl group of P1by anester bond and insertion of the N-terminal part of the RCLas strand 4 in b-sheet A (s4A) of the serpin. ... behaviour of the serpin.Complex formation between serpins and their cognateproteases is fuelled by the thermodynamic properties of the serpin. Accordingly, insertion of the RCL as s4A and the ensuing ... that the aliphatic moiety of the introduced K in PAI-1(M149K)allows the side chain to adapt the same orientation as the original M and therefore the stabilizing effect of the M149Ksubstitution...

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