... In the present study, we compared the functional roles of the N- and C-terminal regions of molluskan and vertebrate TnI and revealed for the first time that (a) the alternative binding of the ... Lanes a and d, in the absence of both TnC and Ca2+; lanes b and e, in the presence of TnC and the absence of Ca2+; lanes c and f, in the presence of bothTnC and Ca2+. Ac, actin; Tm, tropomyosin; ... ATnI130)252, and ATnI232)292did not migrate into the gels, while free ATnI-52K and ATnI1)128exhibited a band near the origin and at the middle of the gel, respectively. The bands corresponding to the...