Tài liệu Báo cáo khoa học: Functional studies of active-site mutants from Drosophila melanogaster deoxyribonucleoside kinase Investigations of the putative catalytic glutamate–arginine pair and of residues responsible for substrate specificity docx

... from Drosophila melanogaster deoxyribonucleoside kinase Investigations of the putative catalytic glutamate–arginine pair and of residues responsible for substrate specificity Louise Egeblad-Welin 1,2,* , ... (BVDU). Mutants of catalytic residues: E52 and R105 It is evident from the kinetic results that mutation of E52 changes only the cat...

Ngày tải lên: 19/02/2014, 02:20

... In the present study, we compared the functional roles of the N- and C-terminal regions of molluskan and vertebrate TnI and revealed for the first time that (a) the alternative binding of the ... Lanes a and d, in the absence of both TnC and Ca 2+ ; lanes b and e, in the presence of TnC and the absence of Ca 2+ ; lanes c and f, in the presenc...

Ngày tải lên: 20/02/2014, 01:20

... probes are useful for both studying and controlling the functions of enzymes and other proteins. The most useful probes have high affinity for their target, along with small size and resistance to ... 123, 2937–2945. 50 Burlingham BT (2002) Design and synthesis of chemical probes for the investigation of enzyme recognition of anionic substrates. PhD Thesis, Departme...

Ngày tải lên: 14/02/2014, 22:20

... is assigned to the mixing of the 4p orbital with the 3d orbital of the metal cofactor. Two important pieces of information can be gleaned from the pre-edge peak. First, occurrence of this tran- sition ... with one of the initial models considered (fit 1). See Table S1 for further details of EXAFS data analysis. N is the number of ligands, r is the distance to...

Ngày tải lên: 18/02/2014, 06:20

... that the rate of irreversible unfolding for VsEndA is higher than for Vc EndA, with a half-life of % 13 and 33 min, respectively. Substrate specificity analysis An analysis of the substrate specificity ... specificity for DNA of the enzymes shows that they both cleave plasmid DNA, dsDNA and ssDNA as shown in Fig. 8. To test the RNA specificity of the enzy...

Ngày tải lên: 19/02/2014, 05:20

... deletion of 9 3 residues (DEC1: 94–412) or more than 93 residues ( DEC1:119–412) abolished the suppression (Fig. 1A). On the other h and, deletion of up to 273 residues from the C-terminal region of ... binding to the E-box (lanes 6 and 16). On t he other hand, deletion of up to 273 residues from the C-terminal region of DEC1 (VP16-DEC1:4–232 and VP16-DEC...

Ngày tải lên: 19/02/2014, 16:20

... inhibit the LBS function of either K1 [34] or K4 [35] and have used them to analyse, in functional isolation, the role of these LBS on plas- min(ogen) binding and activation. The effect of these mAbs ... (inset) in the presence of varying concentrations of mAb A10.2 or mAb 34D3 (0–1 l M for cells and 0–0.25 lM for fibrin) and fixed concentrations of Glu-Pg (200...

Ngày tải lên: 20/02/2014, 01:20

... component of 60S ribosome large subunit. The ATPase activity of Gua is important for this interaction and is also necessary for the func- tion of Gua in the production of both 18S and 28S rRNAs. ... The observed difference in the sensitivity of the two experiments (Fig. 2A,B) might be attributed to difference in the levels of expression of FLAG-Gua and FL...

Ngày tải lên: 20/02/2014, 01:20

... crossover – and the unpairing of four base-pairs in the centre of the junctions [19]. Detailed understanding of the functional role of these interactions would require the crystal structure of the Ydc2–junction complex. In ... Binding of CCE1 results in the complete unpairing of the four base-pairs in the centre of the junctions with a lesser disruption...

Ngày tải lên: 20/02/2014, 11:20

... C of the core domain and contains the protruding antiparallel coiled-coil motif (helices 10 and 11, residues 187–203 and 208–226) responsible for the dimerization of EF-Ts in the crystal. In the ... the present results indicate that the maximal rate of the mutant EF-Ts in protein synthesis in vivo is  70%ofthatofthe wild-type EF-Ts. The studies of the...

Ngày tải lên: 21/02/2014, 00:20