Tài liệu Báo cáo khoa học: Tissue expression and biochemical characterization of human 2-amino 3-carboxymuconate 6-semialdehyde decarboxylase, a key enzyme in tryptophan catabolism pptx

... primer and probe 1 ⁄ 3fw TGGCCAGATCTAAAAAAGAGGT 2fw ATCCCAGGAAACACCAGTAGA 10rev ATTGTTTTCTCTCAAGACCCAA TaqMan probe T1 ACACCACAGCAAGGGAGAAGCAAAG 18Sfw CGCCGCTAGAGGTGAAATTC 18Srev TCTTGGCAAATGCTTTCGCT TaqMan ... kit (Stratagene, La Jolla, CA, USA). Mutagenic primers were: 5¢-CGCTCGAGA TGAAAATTGACATC GCTAGTCATATTCTACC-3¢ and its complement for His6Ala; 5¢-GACATCCATAGT GCT ATTCTACCAAAAGAATGG...

Ngày tải lên: 19/02/2014, 02:20

... majus, Bromhaedia finlaysonia, Arabidopsis thaliana, Persea americana, Ipomea purpurea, Ipomea nil and Medicago sativa), three anthocyanidin synthases (Zea mays, Anthirrhinum majus and Oryza sativa), ... doi:10.1046/j.1432-1033.2002.03108.x Several intermolecular dioxygenases, particularly those of microbial or human origin, catalyze reactions of medicinal and industrial relevance,...

Ngày tải lên: 21/02/2014, 03:20

... 275–283. 10 Kuwada M, Teramoto T, Kumagaye KY, Nakajima K, Watanabe T, Kawai T, Kawakami Y, Niidome T, Sawada K, Nishizawa Y et al. (1994) Omega-agatoxin- TK containing D-serine at position 46, but ... 2008) doi:10.1111/j.1742-4658.2008.06352.x Cone snails, a group of gastropod animals that inhabit tropical seas, are capable of producing a mixture of peptide neurotoxins, namely cono...

Ngày tải lên: 18/02/2014, 17:20

... shortest functional domain from a crenarchaeal plasmid endowed with DNA and RNA synthesis and terminal transferase activity. Abbreviations AEP, archaeo-eukaryotic replicative primases; dNTP, deoxyribonucleotide; ... replicative primases (AEPs) [11]. Primase–polymerases (prim–pols) are a novel family of AEPs which are sporadically found in both bacterio- phages and crenarchaeal...

Ngày tải lên: 18/02/2014, 18:20

... as substrate in PCR with the following Ras-GRF1 gene-speci- fic primers: ON357, 5¢-TGAAACATCACCAACTAAATC TCCAA-3¢; ON358, 5¢-GACGACTCCATTGTTATAGG AAAAGAGT-3¢; ON359, 5 ¢-GCCGCTGGAGAAACAG CAT-3¢; ON360, ... can induce the activation of intracellular cascades such as the mitogen-activated protein (MAP) kinase – also called extracellular signal-regulated kinase (ERK) – cascade. The serine ⁄ t...

Ngày tải lên: 19/02/2014, 18:20

... D not containing glycine and FTHF. Such an enzyme was once again incubated with glycine (10 m M) and differ- ent concentrations of FTHF as in (j) and the absorbance was mea- sured at 500 nm. ... is at a distance of 2.95 A ˚ from the a- carbon atom of Gly and appears to be suitable for this proton transfer. As the internal and external aldimines of SHMTs have simi- l...

Ngày tải lên: 18/02/2014, 16:20

... overlap affecting the corresponding signals. Correlations were calculated by means of MATHEMATICA 5.2 software, using the relaxation dataset given in supplementary Table S2. Relaxation data obtained ... Universita ` di Udine, Italy Homeodomains (HDs) comprise a very well-known class of DNA-binding domains occurring in a large family of transcription activators involved in the...

Ngày tải lên: 18/02/2014, 16:20

... N-terminal domain and is comparable to that of human defensins and human lysozyme. Elafin and trappin-2 are both antimicrobial against S. aureus and P. aeruginosa [14,15] but not against E. coli ... analysis of elafin and trappin-2 fractionated on heparin-Sepharose. The heparin-binding capacities of elafin and trappin-2 were evaluated by affinity chromatography using heparin...

Ngày tải lên: 18/02/2014, 17:20

... phosphatase 2A interacts with the 70-kDa S6 kinase and is activated by inhibition of FKBP12-rapamycinassociated protein. Proc Natl Acad Sci USA 96, 4438–4442. 11 Petritsch C, Beug H, Balmain A & ... stimulated for various times, fixed and stained with an antibody against the C-terminus of S6K1. Using an fluoroscein isothiocyanate (FITC)-labeled secondary anti-rabbit IgG and pha...

Ngày tải lên: 19/02/2014, 07:20

... Helena Yusuf-Makagiansar 3 , Vincent T. K. Chow 2 , Teruna J. Siahaan 3 and Seetharama D. S. Jois 1 1 Department of Pharmacy and 2 Department of Microbiology, National University of Singapore, Singapore; 3 Department ... T-leukemia and the human colon adenocarcinoma (Caco-2) cell lines were obtained from the American Type Culture Collection (Rockville, MD, USA). Jurkat and M O...

Ngày tải lên: 19/02/2014, 13:20