Tài liệu Báo cáo khoa học: N-terminal extension of the yeast IA3 aspartic proteinase inhibitor relaxes the strict intrinsic selectivity ppt

... (2007) 3685–3694 ª 2007 The Authors Journal compilation ª 2007 FEBS N-terminal extension of the yeast IA 3 aspartic proteinase inhibitor relaxes the strict intrinsic selectivity Tim J. Winterburn 1 , ... of the active site of the target proteinase by the inhibitory segment. For ease of interpretation, residues in the inhibitors are denoted by single...

Ngày tải lên: 19/02/2014, 00:20

... groups. The one exception to this is the location of the amide group of Lys204 at P3, where there is a break in the main-chain density at the 2.8r map level. The corresponding position in the apo ... primarily occurs by the formation of a hydrogen bond between the side-chain hydroxyl group of Tyr205 and the importin-a main chain at the C-terminus of the ARM1...

Ngày tải lên: 14/02/2014, 19:20

... epi- demiological characterization of the virus. These results provide a demonstration of the high projected sensitivity of human-blind dengue primers and the operation of the primer cocktail strategy ... in the row associated with that serotype covered by the primers of the group associated with that column; the primers are categorized according to the strains tha...

Ngày tải lên: 14/02/2014, 19:20

... Kristjansson and Magnusson [62] reached the same conclusion from their study of the effects on lyotropic salts on the stability of Vibrio proteinase, proteinase K and the thermophilic homologue, aqua- lysin ... experimental results on the effects of lyotropic salts on the conformational stability of the Vibrio proteinase, proteinase K and the thermophilic relativ...

Ngày tải lên: 19/02/2014, 16:20

... under- standing of the molecular mode of action of glypho- sate and the molecular basis for glyphosate resistance. Structure and function of EPSPS EPSPS catalyzes the transfer of the enolpyruvyl moiety of ... however, was devoid of substrate or inhibitor, and consequently did not reveal the nature of the active site or the mode of action of glyphosate. A de...

Ngày tải lên: 14/02/2014, 14:20

... whether the tyrosine phosphorylation status of tau also affects the binding of tau to Fyn-SH3, CHO cells were cotransfected with Fyn together with either wild-type or the YF mutant forms of tau. ... models of AD [8], suggesting that disruption of the association of these proteins could represent a poten- tial therapeutic strategy for the treatment of AD. Here, we used...

Ngày tải lên: 14/02/2014, 14:20

... on the surface of the molecule and is rather far away from the trinuclear site. Therefore, the C-terminal sequence of RlL might be more of an evolu- tionary relic than a functional feature of the ... also rather thermostable as compared with many other laccases. The stabilization of both the N-termini and C-termini of TaLcc1 and MaL might be a reason for the higher...

Ngày tải lên: 14/02/2014, 18:20

... ligand, NE2 of His31, bound to the metal, in the company of a water oxygen, from the apical positions. The manga- nese was only 0.06 A ˚ out of the equatorial plane (Table 3). The angles around the metal ... counted the interactions, we excluded all residues involved in the binding of the metal cofactor. ApeSODs in different forms were superimposed with the least...

Ngày tải lên: 14/02/2014, 22:20

... to the catalytic site of the R1E subunit. As reported in the Results, upon completion of substrate conversion, the radical is then rapidly passed back from the R1E subunit to the tyrosyl of the ... RNR and that of other class 1 RNRs or, instead, is a result of the interaction of the R2F with the inactivated (oxidized) form of the R1E. We consider the first...

Ngày tải lên: 15/02/2014, 01:20

... of the G194P were lower than those of the WT enzyme (Table 2). As the structure of the G194P mutant become more stable than that of the WT enzyme, the rmsf values (calculated by the MDS) of the residues ... whereas the T 50 of G198P was lower than that of the WT enzyme. The T 50 of the double point mutant (G194P ⁄ G198P) was similar to that of the W...

Ngày tải lên: 15/02/2014, 01:20