Tài liệu Báo cáo khoa học: Complete reconstitution of an ATP-binding cassette transporter LolCDE complex from separately isolated subunits docx

... Complete reconstitution of an ATP-binding cassette transporter LolCDE complex from separately isolated subunits Kyoko Kanamaru* , , Naohiro Taniguchi, Shigehiko Miyamoto, ... complete reconstitution system of the LolCDE complex from isolated subunits, it seemed important to examine in detail the stability of LolD and LolCDE in a DDM solution. The...

Ngày tải lên: 19/02/2014, 00:20

... Kruus 2 , Juha Rouvinen 1 and Nina Hakulinen 1 1 Department of Chemistry, University of Eastern Finland, Joensuu, Finland 2 VTT Technical Research Centre of Finland, Espoo, Finland Keywords ascomycete; ... surface of the molecule and is rather far away from the trinuclear site. Therefore, the C-terminal sequence of RlL might be more of an evolu- tionary relic than a functional...

Ngày tải lên: 14/02/2014, 18:20

... nitro- gen(oxygen) donor ligands at an average distance of 1.90 A ˚ and four nitrogen(oxygen) ligands at an average distance of 2.06 A ˚ . Three of the four ligands were reported to be consistent ... formation of the ARD–acireductone complex, the best fit of the EXAFS suggested three nitrogen(oxygen) donor ligands at an average distance of 1.92 A ˚ and three nitrogen(oxygen)...

Ngày tải lên: 18/02/2014, 06:20

... opposite sides of the b-sheet. From the topology diagram of BcZBP (Fig. 1A), an active site can be predicted in the crevice adjacent to the C-termini of strands b1 and b4. In this crevice of each monomer, ... polypeptide chain of 234 amino acids (UniProt accession number Q81FP2) and has three homologues in B. anthracis (str. A2012) (i.e. bant_01002171, bant_01004539 and bant_010...

Ngày tải lên: 19/02/2014, 00:20

... chain. A final set of restraints consisting of 580 non-redundant interproton distances and 24 / dihedral angles was derived from spectral analysis, Table 1. A plot showing the total number of NOE-derived ... dimerization of troponin C: mode of interaction and use of trifluoroethanol as a denaturant of quaternary structure. Biochemistry 34, 7365–7375. 45. Endo, T., Oya, M., Ozawa...

Ngày tải lên: 20/02/2014, 11:20

... in malignant tissues [28]. Elucidation of how the expression of Mcm protein changes relates to malignant transformation of cells and the pathophysiological signifi- cance of those changes awaits ... NaCl/P i for 30 min and incubated with each of the following primary antibodies at 4 °C overnight: rabbit anti-Mcm3, anti-Mcm4 and anti- Ki-67, and mouse anti-PCNA IgG. These antibodies w...

Ngày tải lên: 20/02/2014, 23:20

... Bayesian posterior probability (0.87) for an association of the myriapod hemocyanins with the crustacean and insect hemocyanins. All analyses support a pancrustacean taxon of the insect and crustacean ... Beintema, J.J. (1988) Panulirus interruptus hemocyanin: the amino acid sequence of subunit b and anomalous behaviour of subunits a and b on polyacrylamide gel electrophoresis in...

Ngày tải lên: 20/02/2014, 11:20

... most glyphosate-resistant biotypes. In the case of Conyza canadensis, glyphosate accumulates in vacuoles of resis- tant plants at a markedly faster rate than in sensitive plants [61]. Analysis of the transcriptome ... modification of an existing enzyme as guided by a structural model; and (c) modification of an exist- ing enzyme by gene shuffling and selection. Each approach has it...

Ngày tải lên: 14/02/2014, 14:20

... pervanadate induced the appearance of an  68-kDa band in wild-type, Y18F, Y29F, Y197F and Y310F tau, this band was not apparent on western blots of lysates of Y394F tau (Fig. 2A). The absence of ... revealed decreased electrophoretic mobility of tau, with the appearance of an  68-kDa tau species in wild-type and all of the mutant forms of tau except for YallF and Y394F t...

Ngày tải lên: 14/02/2014, 14:20

... was transformed into BL21 (DE3) cells and grown at 37 °C overnight in Luria–Bertani medium containing 30 lgÆmL )1 of kanamycin. Flasks of 400 mL of Luria– Bertani medium (30 lgÆmL )1 kanamycin) ... nuclear transport factor-2 path- way is normally used for the nuclear import of RanGDP [28], but nuclear transport factor-2 can also interact with cargo complexes [29]. The nuclear trans- p...

Ngày tải lên: 14/02/2014, 19:20