Tài liệu Báo cáo khoa học: Compartmentalization and in vivo insulin-induced translocation of the insulin-signaling inhibitor Grb14 in rat liver pptx

... domain of the IR in complex with the PIR–BPS of Grb14 and of the Grb14 SH2 domain have allowed us to propose a model for the Grb14 IR interaction. The PIR–BPS binds as a pseudosubstrate inhibitor ... translocation of Grb14 in rat liver FEBS Journal 275 (2008) 4363–4377 ª 2008 The Authors Journal compilation ª 2008 FEBS 4377 Compartmentalization and...

Ngày tải lên: 18/02/2014, 18:20

... coupled assay. In the presence of the reduced Fd1 and acetyl- CoA, the HE-TPP radical intermediate was formed (Figs 3F and 4B), indicating the intermediacy of the HE-TPP radical in both the oxidative and ... under- standing of the metabolism of H. thermophilus. Indeed, this would enable the kinetic analysis of the POR reac- tions in both directions. In...

Ngày tải lên: 16/02/2014, 09:20

... the numbering of the amino- acid sequences of immunoglobulins and the definition of their CDR loops, as described from 1997 [13]. The number- ing and definition of the L-CDR3 and H-CDR3 loops of mAb4E11 ... on anti- bodies and their genes, and performed a systematic scanning of the CDR3 loops of mAb4E11 by mutagen- esis of their residues into alanine (Ala s...

Ngày tải lên: 19/02/2014, 07:20

... containing minichromosomes Treatment of oocytes with deacetylase inhibitors such as TSA may change the bulk acetylation pattern of histones, and in this way alter the structure of chromatin incorpor- ating ... with the topology changes resulting from the loss of 1.5 superhelical turns of the DNA over the whole construct, suggest that the chromatin is indeed more re...

Ngày tải lên: 19/02/2014, 12:20

... acid, arginine catabolism to NO and polyamines NG, aorta endothelial cells Low affinity transporter and arginase share the control of the fluxes through the pathways involving arginine as a precurser of nitric oxide and polyamines ODE ... computational modeling. Here, computational modeling does not refer to statistical models or models of data mining but rather to a mathemati...

Ngày tải lên: 14/02/2014, 14:20

... [238–240]. Even in the absence of pro- tein–protein interactions, factors can assist each other’s binding to DNA within nucleosomes. This principle is illustrated in the model shown in Fig. 7A. In this ... varia- tion and complexity in the number of nucleosomes dis- rupted, the histone composition of the remaining nucleosomes, and the number and nature of p...

Ngày tải lên: 14/02/2014, 18:20

... repressing the indicated targets and presumably hundreds of other as yet unidentified targets. (B) miR-34a and the miR-34a-binding site in the 3¢ UTR of genes shown in (A). miRNAs and cell signaling ... fac- tor induced the expression of miR-221 and miR-222 in PC12 cells, and that this induction is dependent on sustained activation of the ERK1 ⁄ 2 pathway [20]...

Ngày tải lên: 14/02/2014, 19:20

... (RISC)-loading complex (RLC) in an ATP-dependent manner [20]. Next, one strand (the passenger strand) of the miRNA is removed from the RLC, whereas the other strand (the guide strand) remains in the ... both the promoter of the host gene and the promoter in the latter CpG island. The methyla- tion levels of the CpG island are inversely correlated with matur...

Ngày tải lên: 14/02/2014, 19:20

... proteins involved in fibrosis, including multiple collagens, fibrillins and elastin [75]. miR-29 is dramatically repressed in the border zone flanking the infracted area in the mouse model of MI. ... novel insight into the molecular understanding of cardiac excitability. However, considering the inherent capacity of miR- NAs to target a broad range of proteins, the li...

Ngày tải lên: 14/02/2014, 19:20

... [46]. The similarity in the K m values is attributable to the uracil moiety binding in the same fashion [38], which could trigger the initial binding of both substrates. In UpG, the binding of the guanine ... studies of the structure and binding sites of enzymes. 8. Inhibitor binding to ribonucleases. J Mol Biol 45, 491–511. 33 Ui N (1971) Isoelectric points...

Ngày tải lên: 14/02/2014, 22:20