Tài liệu Báo cáo khoa học: Kinetic characterization of the first step of the ribozyme-catalyzed trans excision-splicing reaction docx

... isolate the first reaction step (substrate cleavage) by preventing the second reaction step (exon ligation). In addition, the product of the first reaction step is actually the intermediate in the ... 2008 The Authors Journal compilation ª 2008 FEBS Kinetic characterization of the first step of the ribozyme-catalyzed trans excision-splic...

Ngày tải lên: 18/02/2014, 18:20

... Scheme of the three-hybrid strategy used in the screen. (B) Number of colonies identified in each step of the screen. (C) Distribution of the 63 distinct sequences in relation of their position in the ... similar to that of other well- characterized members of PUF family. These proteins must bind to transcripts in the plant and regulate their translation and ⁄ or tu...

Ngày tải lên: 18/02/2014, 06:20

... superoxide in the course of the reaction. The principle of the assay is that the presence of a SOD inhibits the reduction of cytochrome c. This was vali- dated by adding a total of 2.5 U of commercial ... (1971) The kinetics and mechanisms of the reactions of iron(III) with b-dik- etones. The formation of monoacetylacetoatoiron(III) and the effect of c...

Ngày tải lên: 18/02/2014, 06:20

... the titrant. The active site concentration of wild-type BuChE was 0.16 ± 0.01 lm, and that of the D70G mutant was 0.196 ± 0.02 lm. Effects of ligands on steady-state kinetics The effects of the ... 1) and therefore b ⁄ a >3. The difference in the extent of activation can be explained by differences in the rate-limiting steps. Because the rate-limiting step for...

Ngày tải lên: 18/02/2014, 17:20

... con- centration of 4 mm, HpSDH displayed no activity, either in the presence of NADP or NAD. In compar- ison with the kinetic parameters of SDH enzymes from the other bacteria shown in Table 1 [23,26,27], the ... K m values of HpSDH are similar to those of A. fulgidus SDH (AfSDH), but the k cat value of HpSDH is the lowest, thus the catalytic efficiency of HpSDH i...

Ngày tải lên: 19/02/2014, 05:20

... information on the heme pocket structure. As shown in Table 2, the hyperfine splitting constants of the 15 N nucleus of the distal NO and of the 14 N nucleus of the proximal His of the GmHO-1 complex ... characteristics of the heme pocket of GmHO-1 might be associated with the smaller values of K azide for GmHO-1 and SynHO-1, and with the ferric character o...

Ngày tải lên: 19/02/2014, 05:20

... interactions of apolar resi- dues in the complex, and further propose that the ther- modynamic basis of the binding of all chemokine ligands to their receptor N-domains is similar to that observed for the ... to the receptor CXCR1 N-domain. The binding isotherm of IL-8(1–66) and the trapped L25NMe monomers to the CXCR1 N-domain are shown in Fig. 2. The upper panels s...

Ngày tải lên: 19/02/2014, 05:20

... 4 °C. The supernatant was transferred to an HPLC vial. The products of the reaction were detected at 260 nm using the HPLC system described above for the synthesis of the CoA- esters. The assay ... deficiencies of each of the six dif- ferent steps within the leucine degradation pathway (Fig. 1) cause their own characteristic disease [1]. The enzymes of this path...

Ngày tải lên: 19/02/2014, 07:20

... Myco- plasma penetrans and Spiroplasma kunkelii, the genomes of the members of the mollicutes group (total number, 7 out of 10) are devoid of genes for the biosynthesis of IPP and DMAPP. The same is ... of the enzyme for that cofactor and the apparent absence of a redox process as part of the catalytic cycle. The substrate binding site of the type II enzy...

Ngày tải lên: 19/02/2014, 13:20

... symmetry axis. The labe- led specialized large and elongated cells of the anter- ior part of the floor-plate were positioned at the base of the commissure separating the two lobes of the mesencephalic ... between them, in the central portion of the fin, or in the epithelial cell layer surrounding the fin (Fig. 6C, insert). Lateral line neuromasts of the head r...

Ngày tải lên: 19/02/2014, 16:20