... con-centration of 4 mm, HpSDH displayed no activity,either in the presence of NADP or NAD. In compar-ison with the kinetic parameters of SDH enzymes from the other bacteria shown in Table 1 [23,26,27], the ... Kmvalues of HpSDH are similar to those of A. fulgidusSDH (AfSDH), but the kcatvalue of HpSDH is the lowest, thus the catalytic efficiency of HpSDH is lowerthan those of other SDHs. Notably, the ... system. The enzymatic characterization of HpSDH demonstrates its activity with kcat of 7.7 s)1and Km of 0.148 mmtoward shikimate, kcat of 7.1 s)1and Km of 0.182 mm toward NADP, kcat of 5.2...