Tài liệu Báo cáo khoa học: Purification and structural characterization of a D-amino acid-containing conopeptide, conomarphin, from Conus marmoreus docx

... The Authors Journal compilation ª 2008 FEBS 1987 Purification and structural characterization of a D-amino acid-containing conopeptide, conomarphin, from Conus marmoreus Yuhong Han 1,2, *, Feijuan ... D-amino acid in peptide link- age by an enzyme from frog skin secretions. Proc Natl Acad Sci USA 102, 4235–4239. 33 Shikata Y, Watanabe T, Teramoto T, Inoue A, Kaw...

Ngày tải lên: 18/02/2014, 17:20

... ATCCTCACGAACAAGCAG 5RACR GATCGCGATGCAGGCCTT FLF1 GGACGACTACAGCGTCTTCAGTAGA FLR1 TCCAAACAGTCAGTTTCTTAACCGT Table 1. Purification of cellulase from abalone Haliotis discus hannai. Oneunitofcellulasewasdefinedastheamountofenzymethatliberates reducing ... Double-stranded cDNA was syn- thesized from the mRNA with a cDNA synthesis kit (TaKaRa, Tokyo, Japan) and used as an abalone cDNA lib...

Ngày tải lên: 20/02/2014, 23:20

... shortest functional domain from a crenarchaeal plasmid endowed with DNA and RNA synthesis and terminal transferase activity. Abbreviations AEP, archaeo-eukaryotic replicative primases; dNTP, deoxyribonucleotide; ... replicative primases (AEPs) [11]. Primase–polymerases (prim–pols) are a novel family of AEPs which are sporadically found in both bacterio- phages and crenarchaeal...

Ngày tải lên: 18/02/2014, 18:20

... Purification and partial characterization of seven glutathione S -transferase isoforms from the clam Ruditapes decussatus Pascal Hoarau, Ginette Garello, Mauricette Gnassia-Barelli, Miche ` le ... ability to catalyze the conjugation of glutathione to a broad range of electrophilic substrates in animal organisms. These classes are named alpha, mu, pi, theta, sigma, kappa, zeta...

Ngày tải lên: 31/03/2014, 09:20

... sialic acid. Haemophilus influenzae remains an important cause of disease worldwide. Encapsulated strains can cause invasive, bacteraemic infections such as septicemia and strains lacking a capsule, ... of particular importance in strains that lack a capsular structure, which in itself provides serum resistance. Recent data from our laboratory indicate that the ability of acapsul...

Ngày tải lên: 23/03/2014, 21:21

... primers; CLGAT 5a (5¢-GGCATCAACAT- CACAAGCAACAAGTGCAAG-3¢), CLGAT5b (5¢-TCCT- CCATCAATCTGCTTCCATGGACCATC-3¢), CLGAT 3a (5¢-GCTGTGGCTACGAATGAGGCCGCC-3) and CLGAT3b (5¢-AAGGGAGAGAAACCTGACCTTGCACTTG-3¢). ... watermelon Kentaro Takahara, Kinya Akashi and Akiho Yokota Graduate School of Biological Sciences, Nara Institute of Science and Technology, Japan Drought in the presence of st...

Ngày tải lên: 20/02/2014, 03:20

... Purification and characterization of a sialic acid specific lectin from the hemolymph of the freshwater crab Paratelphusa jacquemontii Maghil Denis, P. D. Mercy Palatty, N. Renuka Bai and S. ... sialidasetypeX,protease enzymes and molecular mass standards were purchased from Sigma. Preparation of crab sera Freshwater field crabs, Paratelphusa jacquemontii were collected fr...

Ngày tải lên: 21/02/2014, 00:20

... with an apparent molecular mass of 53 kDa appears as a double band in unboiled samples (lanes A1 and B1). Table 1. N-Terminal sequences of the polypeptides of the purified enzyme. N-Terminal sequen ... of AF499 was identified as an archaeal promoter element by seque nce analysis. The sequ ence AAAGGTTAATATA shows a high le vel of identity with th e consensus se quence ()35 to )...

Ngày tải lên: 21/02/2014, 03:20

... primer and probe 1 ⁄ 3fw TGGCCAGATCTAAAAAAGAGGT 2fw ATCCCAGGAAACACCAGTAGA 10rev ATTGTTTTCTCTCAAGACCCAA TaqMan probe T1 ACACCACAGCAAGGGAGAAGCAAAG 18Sfw CGCCGCTAGAGGTGAAATTC 18Srev TCTTGGCAAATGCTTTCGCT TaqMan ... fw, forward; rev, reverse. Sequence ACMSD cloning: primer 1fw CGCTCGAGATGAAAATTGACATCCATA GTCAT 11rev AAAGCTGAGCTCCATTCAAATTGTTTT CTCTCAAG 4fw TTCTCGAGATGGGAAAGTCTTCAGAGT GGT ACMSD r...

Ngày tải lên: 19/02/2014, 02:20

... 216 73TTREEQPAAEAAASTAGGSQ ACC ACC AGG GAA GAA CAA CCG GCG GCA GAG GCC GCG GCG TCC ACC GCT GGC GGT AGC CAA 276 93QEGYGSTQLPSDEPLNGLND CAA GAA GGA TAT GGC AGC ACC CAA CTT CCA TCG GAC GAG CCA TTG AAC GGG ... 636 213WKDTTSVGCGRVLCDDKKDT TGG AAG GAC ACC ACC AGC GTC GGA TGC GGA CGC GTC CTC TGC GAC GAC AAG AAG GAC ACC 696 233MIMCSYWPPGNYENQKPY ATG ATC ATG TGC AGC TAC TGG CCG CCG GGC AAC TAT GAA AAC...

Ngày tải lên: 07/03/2014, 12:20