Tài liệu Báo cáo khoa học: Modulation of the enzymatic efficiency of ferredoxin-NADP(H) reductase by the amino acid volume around the catalytic site pptx

... 1B shows the pattern seen after the development of the blot. With increase in time of incubation, the intensity of the band for the monomeric protein decreased, whereas the bands for the higher ... Figure 4A shows the kinetics of aggregation of a-synuclein in the presence of 100 lm MPTP and the effect of 50 lm dopamine on the aggregation process. Dopamine...

Ngày tải lên: 14/02/2014, 19:20

... setting, the ability of the F 0 F 1 -ATP synthase to pump protons out of the matrix represents the only component opposing the action of an uncoupler. On the basis of a recent study by Giorgio ... changes, as the percentage change in the steady-state rate of the pathway divided by the percentage change in the enzyme activity caus- ing the flux change. The...

Ngày tải lên: 14/02/2014, 19:20

... affect sterol biosynthesis dur- ing the formation of a mating projection. Nevertheless, the phenotypes of mutants defective in ergosterol synthesis clearly demonstrate the importance of sterols for ... cleave off the reporter RUra3, which is fused to the PAK. Released RUra3, a modified version of the enzyme Ura3 with an arginine at the extreme N-terminus, is targeted for...

Ngày tải lên: 18/02/2014, 13:20

... Authors Journal compilation ª 2008 FEBS Modulation of the enzymatic efficiency of ferredoxin-NADP(H) reductase by the amino acid volume around the catalytic site Matı ´ as A. Musumeci, Adria ´ n ... in the amino acid volume resulted in a decrease in the catalytic efficiency of the enzyme without altering the protein structure. Our results pr...

Ngày tải lên: 18/02/2014, 17:20

... parasites or cell extracts, gave negli- gible values [7,9,10]. These results could be attributed neither to a deficient uptake of the amino acids by the parasites [12] nor to the presence of ODC ... During the subsequent period, the integration of ‡ 2 units of the transfected plasmid into the parasite genome seemed to occur, as shown by the production of both DN...

Ngày tải lên: 19/02/2014, 07:20

... action of EPA and DHA (Eur. J. Biochem. 271) 4465 Modulation of cyclin D1 synthesis and hyperphosphorylation of Rb by n-3 and n-6 PUFAs Induction of cyclin D1 is one of the e arliest effects of mitogenic ... fatty acids (PUFAs) act in opposition to n-6 PUFAs by modulating various steps of the inflam- matory process. We have investigated the molecular mech- anisms by...

Ngày tải lên: 19/02/2014, 16:20

... [8,9]. One of the interesting features of the plant PSs is the presence of a conserved 24 residue insertion in the sequence [9]. This sequence of amino acids, which is missing in the sequence of bacterial ... [13]. Details of the structural basis for the biochemically observed inhibition of plant PS by pantoate, as well as of the alleviation of this inhib...

Ngày tải lên: 16/02/2014, 09:20

... withdrawal of the hypoxia mimetics. Importantly, knockdown of hypoxia inducible factor-1a by RNA interference restores the differentiation capabilities of the cells, even in the presence of cobalt ... malignance grading, in other words, higher levels of expression of HIF-1a suggest a greater degree of differentiation defects. Then, we sought to test the generality o...

Ngày tải lên: 18/02/2014, 13:20

... hetero- geneous group of enzymes with distinct roles in cell function [1–5]. The common feature of these enzymes is that they all selectively hydrolyze the fatty acid at the sn-2 position of glycerophospholipids. ... The iPLA 2 -VIA effects were found to be independent of the generation of free arachidonic acid or one of its oxygenated metabolites, and may work throu...

Ngày tải lên: 18/02/2014, 17:20

... oct-TKRPR. These results indicated that oct-TKRPR belongs to the class I GPCR family. The total amino acid sequence of the oct-TKRPR is 26.8–43.6% homologous to the sequences of TKR and the TKRPR ... and the importance of C-terminal Arg-NH 2 in the specific recognition of TKRPs by TKRPR is conserved through evolutionary lineages of Octopus. Southern blotting of...

Ngày tải lên: 19/02/2014, 00:20