Tài liệu Báo cáo khoa học: Poly(silicate)-metabolizing silicatein in siliceous spicules and silicasomes of demosponges comprises dual enzymatic activities (silica polymerase and silica esterase) doc

... Poly(silicate)-metabolizing silicatein in siliceous spicules and silicasomes of demosponges comprises dual enzymatic activities (silica polymerase and silica esterase) Werner ... tetrahedral silica units in poly(silicate) is an ester-like bond. In order to test whether silicatein – in addition to being a poly(silicate)-forming enzyme (silica...

Ngày tải lên: 18/02/2014, 16:20

... (2010) Solution structure of the C-terminal X domain of the measles virus phosphoprotein and interaction with the intrinsically disordered C-terminal domain of the nucleoprotein. J Mol Recognit 23, ... C-terminal domain of the measles virus nucleoprotein is intrinsically disordered and folds upon binding to the C-terminal moiety of the phosphopro- tein. J Biol Chem 278, 18638–18...

Ngày tải lên: 18/02/2014, 04:20

... transport and sen- sory system and, in this function, is one of the key players of catabolite repression. Mathematical modelling of signal transduction and gene expres- sion of the enzymes involved in ... uptake and concomitant phosphory- lation of glucose and a number of other carbohydrates (Fig. 1). It consists of two common cytoplasmic pro- teins, enzyme I (EI)...

Ngày tải lên: 18/02/2014, 13:20

... of Bax and tBid [10]. Concluding remarks In summary, peptides corresponding to natural sequences of Bax and encompassing individual helices of the characteristic a5–a6 hairpin domain can inde- pendently, ... hairpin found in the core of water-soluble folds of these proteins has been reported to be the pore- forming domain. Here we show that peptides including any of the tw...

Ngày tải lên: 19/02/2014, 07:20

... dependence of the rate of aggregation on protein concentration, implying that ArgEE prevents intermolecular interactions. The rates of inactivation and aggregation of lysozyme in the presence of ArgEE ... obtained in the presence of NaCl suggest that the molecular mechanism of ArgEE in preventing thermal inactivation and aggregation is different from that of Arg. The...

Ngày tải lên: 19/02/2014, 16:20

... (gradient of 15–150 mm NaCl contained in 1 L buffer A) to obtain pure protein. Redox potentiometry, ligand binding and stopped-flow kinetic analyses Redox titrations and the determination of redox ... unusual in having two histidine residues (rather than the His-Asn pair seen in OYE, MR and some of the other member proteins; Fig. 1B) in the active site for ligand and subs...

Ngày tải lên: 20/02/2014, 02:21

... number of intracellular roles: it can alter gene expression, in uence the rate of cell proliferation and differ- entiation, and inhibit apoptosis in a manner independent of urokinase inhi- bition. ... pattern of PAI-2 has also been shown for a related serpin known as maspin [13] and this feature remains one of the most intriguing aspects of PAI-2 (and maspin) biology. Be...

Ngày tải lên: 20/02/2014, 02:21

... includ- ing fibrin, extracellular matrix components (laminin, fibronectin) and proteins involved in extracellular matrix degradation (matrix metalloproteinases, such as MMP3) [47–50]. Binding of ... threonine and one serine in embryonic kid- ney, three serines and two threonines in leukemia; whereas two tyrosine residues were found in both leu- kemia and lung cancer and one...

Ngày tải lên: 14/02/2014, 19:20

... total binding from non-specific binding, cells were incubated for 10 min in either PBSCM alone or PBSCM containing 200 lm cocaine for inhibition of DAT or 200 lm S-citalopram for inhibition of SERT. ... After incubation with biotin, the cells were washed twice in PBSCM supplied with 100 mm glycine, and incubated for 20 min to quench further cross-linking. The quenching buffer was rem...

Ngày tải lên: 14/02/2014, 21:20

... activity of vInv in Rsch increased continuously during cold exposure, and became significantly higher than in C24 after 7 days at 4 °C. As compared with that of vInv, the activities of nInv and eInv ... metabolic engineering of freezing tolerance in plants should include such an analysis of the dynamics of metabolism to gain com- prehensive information about the effects...

Ngày tải lên: 14/02/2014, 22:20