Tài liệu Báo cáo khoa học: Protein kinase CK2 activates the atypical Rio1p kinase and promotes its cell-cycle phase-dependent degradation in yeast pdf

... is under the direct control of kinases and phosphatases, and the indirect control of phosphorylation through the regula- tion of other apoptotic proteins. Furthermore, many kinases and phosphatases ... and increases the release of cytochrome c from mitochon- dria into the cytosol through inactivation with Bcl-xL and Bcl-2 [69]. Inactivated Bax and Bad bind 14-3-3, the...

Ngày tải lên: 14/02/2014, 22:20

... character- ized by an increase in cell size and protein content in the absence of cell proliferation. In the CNS, indirect evidence exist that UII also activates ERK and Rho -kinase pathways. In rat, cen- trally ... activation of the channels via proteinase kinase A dependent phosphorylation and no involvement of PKC in the process. While the UII receptor was...

Ngày tải lên: 19/02/2014, 07:20

... the protein protein interaction between v-KIND and MAP2 (i.e. the MAP2-binding core module in v-KIND and the v-KIND-binding core module in MAP2) across a range of amino acid resi- dues, and provided ... (RasGEF) very- KIND (v-KIND, or kinase noncatalytic C-lobe domain containing 1) [9]. The KIND domain in these proteins is localized to the N-terminal region, and th...

Ngày tải lên: 14/02/2014, 19:20

... aprotinin, leupeptin and pepstatin, twice in kinase buffer, and used for in vitro kinase assays. In vitro kinase assays Recombinant human protein kinase CK2 holoenzyme (0.5 U, corresponding to ... progression through the G 1 phase [11]. Keywords atypical protein kinase; casein kinase 2 substrate; cell-cycle phase-dependent degradation; protein protein int...

Ngày tải lên: 18/02/2014, 16:20

... protein kinase 1 (DAPK-1) is a Ca 2+ ⁄ calmodulin-regulated serine ⁄ threonine kinase composed of multiple functional domains, including a kinase domain, a calmodulin-binding domain, eight ankyrin ... Furthermore, microtubule-associated protein 1B interaction with the kinase domain of DAPK-1 stimulates membrane bleb- bing and autophagy [12]. The roles of its other func- ti...

Ngày tải lên: 18/02/2014, 17:20

... understanding of the folding mechanism(s) and the factors affecting protein stabilization, in this article we extend our knowledge on anion protein interactions by determining the effect on the protein ... and LS stand for high- and low-spin, and I HH and I HM indicate the bis-histidine and the His-Met coordinated intermediates) we observe that the sulfate ind...

Ngày tải lên: 19/02/2014, 05:20

... proposed to inject the acyl chain into the sub- strate-binding pocket [32]. If a similar mechanism is involved in the KAS I reaction then an intermediate ACP-acyl–KAS I crystal complex remains to ... mutants all point to the orientation of H333 and its hydrogen bond donor properties as being the cornerstone of obtaining and ⁄ or retaining residual decarboxylase activity. Th...

Ngày tải lên: 19/02/2014, 08:20

... dRp assays, and examined the in uence of d ifferent cis-acting elements on binding of NS5B protein and RNA synthesis in vitro. Materials and methods Expression and purification of NS5B proteins The recombinant ... plus-RNA synthesis might be present in the minus-RNA, as well as i n the plus-RNA. To investigate the binding activity of the CSFV NS5B protein on the...

Ngày tải lên: 19/02/2014, 16:20

... 94%, and the former shares 98% and 85% identities with the N-terminal and C-terminal domains of RD3, respectively (for RD2, the corresponding identities are 94% and 77%, respectively). For RD1 and ... fragments of the peak cleaved by TPCK tryp- sin (T1–T5). By comparing the cleaved sequences with each other, the N1 and T1 fragments were assigned to the N-terminal seq...

Ngày tải lên: 19/02/2014, 16:20

... l M chaetomellic acid A (2) and not at all by 180 l M compound 4. In both cases, lengthening the hydrophobic surface of the analog added additional interactions, which increased the binding affinity of the inhibitor ... for the initiator-binding site. The difference in the inhibition of H. brasiliensis and P.argent- atum rubber transferases by chaetomellic acid A indicate...

Ngày tải lên: 21/02/2014, 00:20