Tài liệu Báo cáo khoa học: Structure determination and biochemical studies on Bacillus stearothermophilus E53Q serine hydroxymethyltransferase and its complexes provide insights on function and enzyme memory doc

... determination and biochemical studies on Bacillus stearothermophilus E53Q serine hydroxymethyltransferase and its complexes provide insights on function and enzyme memory V. Rajaram 1 *, B. S. Bhavani 3 *, ... structure of E53QbsSHMT and its complexes V. Rajaram et al. 4160 FEBS Journal 274 (2007) 4148–4160 ª 2007 The Authors Journal compilation...

Ngày tải lên: 18/02/2014, 16:20

... histone acetylation and deacetyla- tion, and transient recruitment of remodellers and transcription factors. A cycle of transcription commences with the recruit- ment of transcription factors and ... assem- ble complexes containing repressive HMT and DNMT proteins that act in a concerted fashion to maintain both DNA methylation and H3-K9 methylation [202]. However, CG islands us...

Ngày tải lên: 14/02/2014, 18:20

... Sa2–3¢-GMP and RNase Sa2–2¢-GMP seems to be in the conformation of the Arg34 side chain, which appears to depend on whether the mononucleotide is in the syn-conformation or anti-conformation. In RNase ... can be a proton donor for the leaving O5¢ RNA strand. Taking into consideration the conformation of both arginine side chains in the RNase Sa2–mononucleotide structures, Arg67 and Arg7...

Ngày tải lên: 18/02/2014, 11:20

... compilation ª 2008 FEBS evolution and herbivore defense in Brassicaceae. Plant Mol Biol 42, 93–113. 5Lo ¨ nnerdal B & Janson JC (1973) Studies on myrosinas- es. II. Purification and characterization ... we optimized the conditions for E. coli cell-free production of At3g16450.1 with regard to reaction temperature, duration of incubation, and expression vector. For com- parison pu...

Ngày tải lên: 18/02/2014, 14:20

... in protein processing or degradation under different conditions and in different stages of plant development suggests some functional specialization. Based on the recent findings on the diversity of A1 family ... protein-processing enzymes responsible for the conversion because, in terms of primary structure organization, plant APs precursors are, in general, very similar. Like for monoc...

Ngày tải lên: 19/02/2014, 12:20

... three-dimensional structures of about half of the known neuronal specific a-conotoxins have now been determined and have a consensus fold containing a helical region braced by two conserved disulfide bonds. These ... structural information and the insights into structure- activity relation- ships of a-conotoxins is presented in this review. Structural features of a-conotoxins The three-d...

Ngày tải lên: 19/02/2014, 12:20

... fusion peptide on its structure, organization and membrane interactions. Biochim. Biophys. Ac ta 1612, 41–51. 21. Tamm, L.K. & Tatulian, S.A. (1993) Orientation of functional and nonfunctional ... membrane. Effects of pH on the secondary structure and membrane interaction of IFP–wt Based o n studies on the molecular clustering of EnvA fusion protein of ASLV i n response...

Ngày tải lên: 19/02/2014, 16:20

... similar conclusion based on extensive sequence comparisons between and within the CPA and CG2 families was recently reported [29]. In addition, one of the ligands for t he more weakly bound zinc ion ... hexameric LAP contains a second, N-terminal, domain involved in subunit contacts. CG2, on the other hand, is dimeric and has a 110 amino-acid insertion that forms a second domain tha...

Ngày tải lên: 21/02/2014, 03:20

... taking consultant as the head, and using the information on the lexical frame CONSULTANT- COMMON (which is inherited from the COMMON- NOUN class) that it requires a determiner as its only complement ... information once thought to be clearly the responsi- bility of phrase structure rules is in fact more simply represented as lexical information, once one has the power of a...

Ngày tải lên: 21/02/2014, 20:20

... unlabeled dopamine or serotonin, respectively. The final concentration of dopamine and serotonin ranged from 0–10 lm. For single V max determinations, only one concentration (5 lm)of[ 3 H]- dopamine ... the 0.1–2.5 mm DA concentration range, and a concentration of 0.5 mm was used for further survival studies. HEK- MSR-293 cells were transfected in a solution with a fixed concentration of v...

Ngày tải lên: 14/02/2014, 21:20