Tài liệu Báo cáo khoa học " ĐÁNH GIÁ KHẢ NĂNG THÔNG HƯỚNG KHI THI CÔNG ĐƯỜNG HẦM BẰNG PHƯƠNG PHÁP ĐÀO HẦM ĐỐI HƯỚNG " pptx

... ĐÁNH GIÁ KHẢ NĂNG THÔNG HƯỚNG KHI THI CÔNG ĐƯỜNG HẦM BẰNG PHƯƠNG PHÁP ĐÀO HẦM ĐỐI HƯỚNG KS. DIÊM CÔNG HUY Viện KHCN Xây dựng Tóm tắt: Trong quy trình thi công đường hầm thì công ... phát hiện. Để đánh giá được khả năng thông hướng của các hướng khi thi công bằng phương pháp đào hầm đối hướng trước hết phải kiểm tra độc lập độ ổn định của các mốc khống chế mặt bằng và độ cao. ... giữa mũi thi công số 1 và số 2 có chiều dài 4 km. Hình 1. Sơ đồ bố trí các mũi thi công đường hầm áp lực b. Phương pháp kiểm tra Để đánh giá được khả năng thông hướng của mũi thi công số...

Tài liệu Báo cáo khoa học: P25a ⁄ TPPP expression increases plasma membrane presentation of the dopamine transporter and enhances cellular sensitivity to dopamine toxicity pptx

... four times inPBSCM before elution with 50 lLof20mm dithioerythrei-tol-containing SDS sample buffer, and incubated for 30 minat 37 °C. The dithioerythreitol reduces the disulﬁde bridgein the ... hypothesized to play a major role in thisselective loss of dopaminergic neurons, and the cyto-solic fraction of DA is considered particularly toxic[3,7,26]. This highlights the importance of ... in the saggital plane was obtainedfresh from a local abattoir and immediately cooled on ice.Within 2 h, the nucleus caudatus, i.e. the part of thep25a regulates dopamine transporter function...

Tài liệu Báo cáo khoa học: Autolytic activity of human calpain 7 is enhanced by ESCRT-III-related protein IST1 through MIT–MIM interaction pptx

... mutagenesisperformed in this study.This supplementary material can be found in theonline version of this article.Please note: As a service to our authors and readers,this journal provides supporting ... GST–IST1L326D,L353Awas induced with 0.5 mm isopropyl thio-b-d-galactoside for3 h at 30 °C, and the proteins were puriﬁed by binding toglutathione–Sepharose 4B beads according to the manufac-turer’s ... immobilized on glutathione–Sepharose beads andmixed with 10 lg of recombinant calpain 7 MIT domainsdiluted in buffer B (50 mm Tris ⁄ HCl, pH 8.0, 350 mm NaCl,0.2% NP-40, 1 mm dithiothreitol) for...

Tài liệu Báo cáo khoa học: The stereochemistry of benzo[a]pyrene-2¢-deoxyguanosine adducts affects DNA methylation by SssI and HhaI DNA methyltransferases pptx

... to changes inthe microenvironment when this adduct is ﬂanked by atarget cytosine that undergoes ﬂipping in the MTase–DNA complexes. In accordance with this, the depend-ence of the ﬂuorescence ... prokaryotic MTases M.HhaI and M.SssI does nothave any signiﬁcant effect on DNA methylation rates.This difference may be associated with the intercalativeconformation of the (+)-cis adduct and ... interactions of the catalytic loopsof the MTases and the minor groove of DNA. Inaccordance with this hypothesis, the ﬂuorescence prop-erties of the pyrenyl residues of the (+)-cis-B[a]P-N2-dG...

Tài liệu Báo cáo khoa học: Changes in purine speciﬁcity in tandem GAF chimeras from cyanobacterial cyaB1 adenylate cyclase and rat phosphodiesterase 2 pptx

... construct III (3.3 lm ). This indicated that theL259V mutation not only reduced cGMP potency butalso interfered with cNMP binding and signalling ingeneral. Apparently, this amino-acid side chain ... has an amino-acid sequence identi-cal with the mouse isoform in this region, and theGAF tandem domain from the cyaB1 AC because thisdomain, despite its sequence identity with that ofcyaB2 (45% ... in coding purine spe-ciﬁcity. The expectation of this hypothesis was fulﬁlledby construct I. A cyaB1 tandem GAF domain withreplacement of this segment by the rPDE2 region,which comprised...

Tài liệu Báo cáo khoa học: Rac upregulates tissue inhibitor of metalloproteinase-1 expression by redox-dependent activation of extracellular signal-regulated kinase signaling pptx

... TIMP-1 pro-moter in HepG2 cells and that this effect is mediatedby Rac [33]. To identify the regulatory elements withinthe TIMP-1 promoter that mediate this effect, we ﬁrsttested different length ... buffer (pH 7.5), containing 150 mm NaCl,10 mm MgCl2, 0.5 mm dithiothreitol, 15 lm ATP, 5 lCi[c-32P]ATP, and 1 mgÆmL)1glutathione S-transferase(GST)–cJun (1–79; Alexis, Gruenberg, Germany) ... H2O2concentrations, and thus catalasemay also affect intracellular signaling by H2O2. This issupported by the fact that both C1199-Tiam1-inducedand V12-Rac1-induced intracellular ROS...

Tài liệu Báo cáo khóa học: The effect of mutations surrounding and within the active site on the catalytic activity of ricin A chain pptx

... that this residueis not necessary for the activity of RTA. This is supportedby the earlier observation showing that deletion of residues258–262 did not inactivate RTA [30]. However, althoughthis ... Unlike this tetranucleotide, the sequence ofthe stem does not appear to be important as long as it isat least three base-pairs in length [8]. Interactions betweenRTA and the rRNA backbone in this ... [10] have beenexamined (Fig. 1). The third model in which the hexanucle-otide is bound to the active site of RTA in an openconformation is not included in this study as, althoughseveral favourable...

Tài liệu Báo cáo khóa học: Trichostatin A reduces hormone-induced transcription of the MMTV promoter and has pleiotropic effects on its chromatin structure pptx

... (A–F) cover thisregion [4], one of which, nucleosome B, covers the DNAsegment around position )60 to )240. This segmentcontains four glucocorticoid response elements (GREs)[4–6]. This whole ... 73–84.34. Vitolo, J.M., Thiriet, C. & Hayes, J.J. (2000) The H3–H4 N-ter-minal tail domains are the primary mediators of transcriptionfactor IIIA access to 5S DNA within a nucleosome. Mol. ... MNase,micrococcal nuclease; MPE, methidiumpropyl-EDTA–Fe(II); NaBu,sodium butyrate; TSA, trichostatin A; TA, triamcinolone acetonide.*Note: Both these authors contributed equally to this work.(Received...

Tài liệu Báo cáo khoa học: Altered expression of CD1d molecules and lipid accumulation in the human hepatoma cell line HepG2 after iron loading pptx

... receptor (thick lines) innonpermeabilized cells cultured without and with iron. Thin linesrepresent background staining with mouse Igs. (B) Histogramsshow background staining with no antibody (thin ... with rab-bit Igs (thick lines) as ﬁrst step in permeabilized cells. (C) Histo-grams show ferritin expression in nonpermeabilized (thin lines,– saponin) and permeabilized cells (thick lines, + ... shows cell surface expression of acrolein adducts in HepG2 cells cul-tured without (thin line, – Fe) or with (thick line, + Fe) iron. Dotted line represents background staining with mouse Igs as...

Tài liệu Báo cáo khoa học: Allosteric modulation of myristate and Mn(III)heme binding to human serum albumin Optical and NMR spectroscopy characterization pptx

... relaxa-tion of the solvent water bulk. This is at differencewith Fe(III)heme, due to the different afﬁnity of themetals for phenolic oxygen ligands [6,8]. Nevertheless,this contribution is not evident ... essentially be regarded as relativedomain rearrangements to the I-II and II-III inter-faces. This allosteric regulation is not observed for shortFAs (e.g. octanoate) that preferably bind to ... displace the speciﬁc ligands (e.g. ibuprofen)without inducing HSA allosteric rearrangement(s).This indicates that the hydrophobic interactionsbetween the long FA polymethylenic tail and HSAdrives...

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