Tài liệu Báo cáo khoa học: Four divergent Arabidopsis ethylene-responsive element-binding factor domains bind to a target DNA motif with a universal CG step core recognition and different flanking bases preference pptx

... The Authors Journal compilation ª 2009 FEBS Four divergent Arabidopsis ethylene-responsive element-binding factor domains bind to a target DNA motif with a universal CG step core recognition and different ... M, Yamaguchi-Shinozaki K & Shinozaki K (1998) Two transcription factors, DREB1 and DREB2, with an EREBP ⁄ AP2 DNA binding domain sep...

Ngày tải lên: 18/02/2014, 13:20

... cleave their substrates after paired basic amino acids, and they are known to participate in the proteolytic activation of a variety of hormones, growth factors, enzymes, recep- tors and viruses, either ... proteases that cleave their substrates at basic amino acids, thereby activating a variety of hormones, growth factors, and viruses. PC1 ⁄ 3, PC2 and PC5 ⁄ 6A are the only me...

Ngày tải lên: 18/02/2014, 16:20

... MPH a Forward: 5¢-TAGAATTCGCTGCTCCACAA GTTAGAACT-3¢ Reverse: 5¢-TA GCGGCCGCTTACTTTGGGTTA ACGACGGA-3¢ Mutant MPH b G194P 5¢-CCTGACGATTCTAAACCGTTCTTCAAGGGTGCC-3¢ G198P 5¢-AAAGGTTTCTTCAAG CCGGCCATGGCTTCCCTT-3¢ G194P ... Each test was carried out with at least three replicates. The K m and k cat val- ues were calculated by nonlinear regression using graphpad prism 5.0 (GraphPad Software Inc...

Ngày tải lên: 15/02/2014, 01:20

... pylori CCUG17874 genomic DNA using the following primers: forward, 5¢-CACCAAACCTTATACGATTGATAAGGCA AAC-3¢; and reverse, 5¢-TTATTATTGGGCGTAAGCT TCTAG-3¢. The construct was cloned directly into the pET151 ... [3]. Major virulence factors that contribute to the inflammatory response and to epithelial cell damage have been iden- tified, among them cytotoxin-associated gene protein A [4,...

Ngày tải lên: 16/02/2014, 14:20

... required. The available data indicate that the accessory factor Bcs1p is involved in the binding of ISP to an immature bc 1 intermediate [28,29] and that Cbp3p and Cbp4p play an essential, but poorly ... and 2) and DQCR9 (lanes 3 and 4) yeast strains were solubilized with 1% digitonin (lanes 1 and 3) or 1% Triton X-100 (lanes 2 and 4) and protein complexes were analyzed...

Ngày tải lên: 18/02/2014, 08:20

... disease. Lancet Neurol 7, 97–109. 4 Hayashi S, Wakabayashi K, Ishikawa A, Nagai H, Sai- to M, Maruyama M, Takahashi T, Ozawa T, Tsuji S & Takahashi H (2000) An autopsy case of autosomal- recessive ... cell- signalling cascades including MAPK and phosphatidylinositol 3-kinase (PI3K) pathways that can target HtrA2, PINK1, Parkin and DJ-1. Likely these PD-associated proteins are par...

Ngày tải lên: 18/02/2014, 14:20

... (Bio- source International, Camarillo, CA, USA) to detect active Akt kinase, as well as a separate Akt polyclonal antibody (Cell Signaling Technology, Danvers, MA, USA) to determine total Akt, as described ... of platelets [26]. The current data indicate that ADP, acting via P2Y 12 and Gi, can down-regulate adenylyl cyclase and hence PKA with consequently increased Ca 2+ mobilizat...

Ngày tải lên: 18/02/2014, 16:20

... The Authors Journal compilation ª 2007 FEBS forms of X-linked mental retardation [4]. In addition, a number of bacterial virulence factors and toxins have been found to target Rho GTPases and to ... panel). HEK293 cells were transfected with a combination of expression plasmids of HA–Rac1L61, HA– Rac1bWT, HA–Rac1bL61 and 6His–myc–Ub as indicated. Proteasomal degradation of R...

Ngày tải lên: 18/02/2014, 16:20

... membrane of Gram-negative bacteria. It activates monocytes and macrophages to produce cytokines such as tumor necrosis factor- a and interleukins IL-1b and IL-6. These cytokines appear to be ... bacteria, and its recognition and signal transmission are key events in the host defense reaction towards Gram-negative bacteria. Generally, LPS activates monocytes and macrophage...

Ngày tải lên: 18/02/2014, 18:20

... ATCCCAGGAAACACCAGTAGA 10rev ATTGTTTTCTCTCAAGACCCAA TaqMan probe T1 ACACCACAGCAAGGGAGAAGCAAAG 18Sfw CGCCGCTAGAGGTGAAATTC 18Srev TCTTGGCAAATGCTTTCGCT TaqMan probe 18S TGGACCGGCGCAAGACGGAC AB Fig. ... primer 1fw CGCTCGAGATGAAAATTGACATCCATA GTCAT 11rev AAAGCTGAGCTCCATTCAAATTGTTTT CTCTCAAG 4fw TTCTCGAGATGGGAAAGTCTTCAGAGT GGT ACMSD real-time PCR: primer and probe 1 ⁄ 3fw TGGCCAGATCTAAAAAAGAGGT 2f...

Ngày tải lên: 19/02/2014, 02:20