... (Bio-source International, Camarillo, CA, USA) to detectactive Akt kinase, as well as a separate Akt polyclonalantibody (Cell Signaling Technology, Danvers, MA, USA) to determine total Akt, as described ... of platelets[26]. The current data indicate that ADP, acting viaP2Y12 and Gi, can down-regulate adenylyl cyclase and hence PKA with consequently increasedCa2+mobilization. This pathway still ... situby activation of PRP with tissue factor and CaCl2,the P2Y12⁄ PI3-K pathway significantly enhances theactivation state and, hence, the procoagulant activityof platelets. Experiments with...