Tài liệu Báo cáo khoa học: Reversible tetramerization of human TK1 to the high catalytic efficient form is induced by pyrophosphate, in addition to tripolyphosphates, or high enzyme concentration ppt

... The Author Journal compilation ª 2008 FEBS Reversible tetramerization of human TK1 to the high catalytic efficient form is induced by pyrophosphate, in addition to tripolyphosphates, or high enzyme concentration Birgitte ... activity is absent in resting cells, appears in late G 1 , increases in S phase coinciding with the increase in...

Ngày tải lên: 18/02/2014, 12:20

... Blunt TOPO PCR Cloning vector (Invitrogen), and the EcoRI fragment was then inserted into the EcoRI site of pCMV3xFLAG-B [46]. Expression vectors for IST1 mutants of MIM were obtained by PCR- based ... to search for potential calpain 7 substrates. As IST1 is involved in cytokinesis rather than endosomal sorting [32,33], calpain might process factors that work in cell divisio...

Ngày tải lên: 18/02/2014, 04:20

... evolutionary selection of protein sequences is the catalytic competence of the enzyme active site. The pre- cise orientation of the functional residues is maintained by a network of interactions that ... 3-phosphate. Enzyme activity was determined by monitoring the decrease in absorbance of NADH at 340 nm. The dependence of the initial rate on the s...

Ngày tải lên: 18/02/2014, 11:20

... using pore-forming toxins for the treatment of solid tumors, which have proven to be quite resistant to conventional toxins [33–35], shows great promise. One of the major drawbacks of using these ... 2. In this study, we investigated the molecular mechanism by which volvatoxin A1 inhibits the cytotoxicity of volvatoxin A2. Volvatoxin A1 by itself was found to be no...

Ngày tải lên: 19/02/2014, 06:20

... appeared. The two isomers seem to interconvert into each other making a separation impossible. Therefore, a mixture of the two isomers had to be used in the following studies. Kinetic constants for ... 5.1) is favored by a factor of 20 over the dehydration reac- tion (k cat ¼ 0.26) which is consistent with the main role of AUH in leucine catabolism, the hydrati...

Ngày tải lên: 19/02/2014, 07:20

... select the top N (= 30) terms from the list L by using a scoring function. For the scoring function of a term x, we use the following function, which is multiplying Nak- agawa’s Imp 1 by a frequency ... processing)” is shown in the column candidate in Table 1. 2.3 Filtering The filtering step is necessary because the obtained candidates are noisy due to the sm...

Ngày tải lên: 20/02/2014, 16:20

... However, the method used by these authors, i.e. the fusion of the receptor to a fluorescent protein, may have affected the conformation of these receptors. Indeed agonist dose–response curves for the ... b 2 -adrenoceptor [3,4], the chemokine receptor CCR5 [5,6], the M 3 muscarinic acetylcholine receptor [7], the M 2 muscarinic cholinergic receptor [8], the melatonin...

Ngày tải lên: 21/02/2014, 00:20

... by nature, in its handling of phosphino- thricin. Organisms that produce this cytotoxic inhibitor of glutamine synthetase have acetyltransferases that derivatize the molecule to a noninhibitory ... restored by muta- tion of Ala100 to glycine, allowing glyphosate to bind in its extended, inhibitory conformation. Detoxification of glyphosate Detoxification of the glyph...

Ngày tải lên: 14/02/2014, 14:20

... domain [20,21]. The SH3 domain of Fyn binds to proline-rich motifs within the sequence PXXP in interacting pro- teins. Seven such motifs are present in the proline-rich domain of the longest isoform ... is important to establish whether or not tau interacts with Fyn-SH2, as this would probably reveal that the tyrosine phosphorylation status of tau is important fo...

Ngày tải lên: 14/02/2014, 14:20

... surrounding the T1 copper form the phenolic substrate-binding site of the enzyme, whereas the T2 and the T3-pair coppers form the trinuclear site that is responsible for binding and reduction of the ... re-organized for online delivery, but are not copy-edited or typeset. Technical support issues arising from supporting information (other than missing files) should b...

Ngày tải lên: 14/02/2014, 18:20