Tài liệu Báo cáo khoa học: Phosphorylation of hormone-sensitive lipase by protein kinase A in vitro promotes an increase in its hydrophobic surface area ppt
Tài liệu Báo cáo khoa học: Phosphorylation of hormone-sensitive lipase by protein kinase A in vitro promotes an increase in its hydrophobic surface area ppt
... FEBS Phosphorylation of hormone-sensitive lipase by protein kinase A in vitro promotes an increase in its hydrophobic surface area Christian Krintel1,2, Matthias Mo¨rgelin3, Derek T. Logan2and Cecilia ... that HSL gainsaccessible hydrophobic surface area upon PKA phos-phorylation. This gain in hydrophobic surface area presumably accounts for the increase in in vitro activity of HSL following ... phospholipids and that this is fol-lowed by an interaction between the apolar acyl chains of the phospholipids and side chains of particularamino acids, thus accounting for the increased capacityto...
- 11
- 562
- 0
Tài liệu Báo cáo khoa học: Phosphorylation of cyclin dependent kinase 4 on tyrosine 17 is mediated by Src family kinases pptx
... 3¢-GTCACAGAGCCACAGTTCCAGCCAGGA-5¢. Codon 305 of C-YES was mutated fromAAA (Lys) to AGA (Arg) with the forward primer: 3¢-GGAACCACGAAAGTAG CAATC AGA ACACTA AAACC A GGTACAATGATGC-5 ¢ . All vector inserts ... resulted in a concomitant reduction in kinase activity as measured by both plate and tubeassays. The accumulation of C-YES in the precipitatescorrelated with the appearance of kinase activity in those ... trichloroacetic acid, andthe proteins were separated using SDS ⁄ PAGE. A major protein band of approximately 60 kDa wasdetected by staining with coomassie (Fig. 2D). Thisband tracked with CDK4 Y17 kinase...
- 11
- 456
- 0
Tài liệu Báo cáo khoa học: Plasticity of laccase generated by homeologous recombination in yeast docx
... SDS–PAGE of purified laccaseenzymes. Lane 1, LAC3; lane 2, LAC131; lane 3, LAC232; lane 4,LAC535; lane M, molecular mass standards (kDa). Each wellcontained 4 lg of protein. Table 1. Apparent kinetic ... report, a similar approach allowedauthors to isolate a variant of a M. thermophila laccasecapable of resisting a wide array of co-solvents at con-centrations as high as 50% v ⁄ v [14]. In all availableexamples ... mutated fungal laccases.Replacement of the aspartic acid D206 by alanine in a Trametes versicolor laccase resulted in a threefold increase in kcat[10]. A similar improvement factor wasalso...
- 10
- 585
- 0
Tài liệu Báo cáo khoa học: Modulation of sterol homeostasis by the Cdc42p effectors Cla4p and Ste20p in the yeast Saccharomyces cerevisiae pptx
... in membranes butalso the amount of sterols derived from SE that areTable 1. Sterol analysis of cells lacking STE20 and CLA4. Data are mean values with standard deviation from at least two independentexperiments.lg ... the in vitro activity of acyl-CoA:ergosterol acyltransferase wasdetermined in an are1 deletion background. Theenzyme activity in the ste20D strain and the cla4D strainwas indistinguishable ... of SE in cla4D cells could beexplained by a negative regulation of Are2p activity by Cla4p. Alternatively, Are2p activity may be normal in cells lacking CLA4, and increased amounts of SE mightsimply...
- 12
- 699
- 0
Tài liệu Báo cáo khoa học: Role of transcription factor activator protein 1 (AP1) in epidermal growth factor-mediated protection against apoptosis induced by a DNA-damaging agent doc
... Bcl-XLexpression, and the resistance against ADR-induced apop-tosis, suggesting that EGF transmitted the antiapoptotic signal in such a way that it activated AP1 via a MAP kinase signaling pathway. TMK-1cells ... signals against ADR-inducedapoptosis by causing activation of the phosphatidylinositol-3¢ -OH kinase (PtdIns3-K) ⁄ Akt signaling pathway in human epithelial cell line MKN74[Takeuchi K & ... times after the ADRaddition and analyzed for caspase 9 activity by using a fluorometricsubstrate-based assay. Each point is the mean of triplicate sam-ples, and the bar represents the standard...
- 13
- 493
- 0
Tài liệu Báo cáo khoa học: Delineation of exoenzyme S residues that mediate the interaction with 14-3-3 and its biological activity ppt
... no intrinsic activity.14-3-3 proteins play an important role in several signa-ling pathways and 14-3-3 interacts with proteins in a phospho-specific manner, using a defined consensus-binding ... vitro. AbbreviationsADPRT, ADP-ribosyltransferase; BD, binding domain; ExoS, exoenzyme S; FAS, factor activating exoenzyme S; GAP, GTPase-activating protein; GEF, guanine exchange factor; GTPase, GTP binding ... frombacteria and transferred to a new Petri dish and incu-bated overnight with medium containing gentamicin.Bacterial growth of each strain was assessed by viablecounts, both during initial infection...
- 9
- 525
- 0
Tài liệu Báo cáo khoa học: Regulation of connective tissue growth factor (CTGF/CCN2) gene transcription and mRNA stability in smooth muscle cells Involvement of RhoA GTPase and p38 MAP kinase and sensitivity to actin dynamics docx
... data are in line with previousobservations indicating that physiological activators of thep38 and PtdIns 3 -kinase pathways including thr ombin,dexamethasone, angiotensin II and prostaglandins ... required an intactcytoskeleton, and, at least in part, the upstream activation of PtdIns 3 -kinase. Inhibition of PtdIns 3 -kinase reducedboth p38 and PKB/Akt phosphorylation in anisomycin-treated ... 38 MAP kinase p athway signals f or cytokine-inducedmRNA stabilization via MAP kinase- activated protein kinase 2and an AU-rich region-targeted mechanism. EMBO J. 18, 4969–4980.50. Dean, J.L.,...
- 15
- 576
- 0
Tài liệu Báo cáo khoa học: "Evaluation of Machine Translations by Reading Comprehension Tests and Subjective Judgments" doc
... EVALUATION OF MACHINE TRANSLATIONS 3 lations than they did the human translations. This measure shows a practice effect in the case of the ma- chine translations, though not for human translations. ... indicate that a good deal of information is available in machine translations, they are also consistent with the view that it is less readily available than in human translations. They also suggest ... of translations during judging affects both types of translations, while loss of context in a paragraph affects only machine trans- lations, is hardly surprising. The range of values of a set...
- 7
- 685
- 1
Tài liệu Báo cáo khoa học: Transcription of individual tRNA1Gly genes from within a multigene family is regulated by transcription factor TFIIIB pdf
... within a multigene family is regulated by transcription factor TFIIIBAkhila Parthasarthy and Karumathil P. GopinathanDepartment of Microbiology and Cell Biology, Indian Institute of Science, Bangalore, ... with increasing concentrations of the unla-belled fragment (lanes 3 and 4, 10 and 100·, respectively); same fragment from which the TATATAA sequence was mutated to GATATCA(lanes 5 and 6, 10 and ... 16,1256–1266.39 Ouyang C, Martinez MJ, Young LS & Sprague KU(2000) TATA-Binding protein TATA interaction is a key determinant of differential transcription of silkwormconstitutive and silk gland-specific...
- 15
- 484
- 0
Tài liệu Báo cáo khoa học: Regulation of DNA fragmentation: the role of caspases and phosphorylation doc
... linkedto the phosphorylation of proteins such as mitochon-drial proteins, caspases, transcriptional factors andnuclear proteins. Phosphorylation of MAPK signaling pathwaysMany protein kinases ... downstream of caspase activation. Many kinases ⁄ phosphata-ses are involved in the control of cell survival and death, including themitogen-activated protein kinase signal transduction pathways. ... Furthermore, manykinases and phosphatases are cleaved by activatedcaspases. The initiator caspase-9 has several sites thatare phosphorylated by multiple protein kinases [88],including the major phosphorylation...
- 15
- 784
- 0
Từ khóa: tài liệu báo cáo nghiên cứu khoa họctài liệu về báo cáo khoa họcbáo cáo khoa học tài chính côngbáo cáo khoa học số loài quý hiếm tại vườn quốc gia ba bểtai lieu bao cao thuc tap khoa co khitai lieu bao cao thuc tap tai khoa duoc benh vienNghiên cứu sự biến đổi một số cytokin ở bệnh nhân xơ cứng bì hệ thốngchuyên đề điện xoay chiều theo dạngMột số giải pháp nâng cao chất lượng streaming thích ứng video trên nền giao thức HTTPNghiên cứu vật liệu biến hóa (metamaterials) hấp thụ sóng điện tử ở vùng tần số THzBiện pháp quản lý hoạt động dạy hát xoan trong trường trung học cơ sở huyện lâm thao, phú thọGiáo án Sinh học 11 bài 13: Thực hành phát hiện diệp lục và carôtenôitNGHIÊN CỨU CÔNG NGHỆ KẾT NỐI VÔ TUYẾN CỰ LY XA, CÔNG SUẤT THẤP LPWAN SLIDEPhát triển mạng lưới kinh doanh nước sạch tại công ty TNHH một thành viên kinh doanh nước sạch quảng ninhNghiên cứu, xây dựng phần mềm smartscan và ứng dụng trong bảo vệ mạng máy tính chuyên dùngNghiên cứu về mô hình thống kê học sâu và ứng dụng trong nhận dạng chữ viết tay hạn chếThơ nôm tứ tuyệt trào phúng hồ xuân hươngThiết kế và chế tạo mô hình biến tần (inverter) cho máy điều hòa không khíKiểm sát việc giải quyết tố giác, tin báo về tội phạm và kiến nghị khởi tố theo pháp luật tố tụng hình sự Việt Nam từ thực tiễn tỉnh Bình Định (Luận văn thạc sĩ)Tăng trưởng tín dụng hộ sản xuất nông nghiệp tại Ngân hàng Nông nghiệp và Phát triển nông thôn Việt Nam chi nhánh tỉnh Bắc Giang (Luận văn thạc sĩ)Giáo án Sinh học 11 bài 15: Tiêu hóa ở động vậtGiáo án Sinh học 11 bài 15: Tiêu hóa ở động vậtGiáo án Sinh học 11 bài 14: Thực hành phát hiện hô hấp ở thực vậtGiáo án Sinh học 11 bài 14: Thực hành phát hiện hô hấp ở thực vậtChiến lược marketing tại ngân hàng Agribank chi nhánh Sài Gòn từ 2013-2015MÔN TRUYỀN THÔNG MARKETING TÍCH HỢP