Tài liệu Báo cáo khoa học: Phosphorylation of hormone-sensitive lipase by protein kinase A in vitro promotes an increase in its hydrophobic surface area ppt

... FEBS Phosphorylation of hormone-sensitive lipase by protein kinase A in vitro promotes an increase in its hydrophobic surface area Christian Krintel 1,2 , Matthias Mo ¨ rgelin 3 , Derek T. Logan 2 and Cecilia ... that HSL gains accessible hydrophobic surface area upon PKA phos- phorylation. This gain in hydrophobic surface area presumably acco...

Ngày tải lên: 18/02/2014, 11:20

... 3¢-GTCACAGAGCCACAGTTCCAG CCAGGA-5¢. Codon 305 of C-YES was mutated from AAA (Lys) to AGA (Arg) with the forward primer: 3¢-GG AACCACGAAAGTAG CAATC AGA ACACTA AAACC A GGTACAATGATGC-5 ¢ . All vector inserts ... resulted in a concomitant reduction in kinase activity as measured by both plate and tube assays. The accumulation of C-YES in the precipitates correlated with the appe...

Ngày tải lên: 18/02/2014, 18:20

... SDS–PAGE of purified laccase enzymes. Lane 1, LAC3; lane 2, LAC131; lane 3, LAC232; lane 4, LAC535; lane M, molecular mass standards (kDa). Each well contained 4 lg of protein. Table 1. Apparent kinetic ... report, a similar approach allowed authors to isolate a variant of a M. thermophila laccase capable of resisting a wide array of co-solvents at con- centrations as high...

Ngày tải lên: 18/02/2014, 06:20

... in membranes but also the amount of sterols derived from SE that are Table 1. Sterol analysis of cells lacking STE20 and CLA4. Data are mean values with standard deviation from at least two independent experiments. lg ... the in vitro activity of acyl-CoA:ergosterol acyltransferase was determined in an are1 deletion background. The enzyme activity in the ste20D strain and th...

Ngày tải lên: 18/02/2014, 13:20

... Bcl-X L expression, and the resistance against ADR-induced apop- tosis, suggesting that EGF transmitted the antiapoptotic signal in such a way that it activated AP1 via a MAP kinase signaling pathway. TMK-1 cells ... signals against ADR-induced apoptosis by causing activation of the phosphatidylinositol-3¢ -OH kinase (PtdIns3-K) ⁄ Akt signaling pathway in human epithelial cell...

Ngày tải lên: 19/02/2014, 06:20

... no intrinsic activity. 14-3-3 proteins play an important role in several signa- ling pathways and 14-3-3 interacts with proteins in a phospho-specific manner, using a defined consensus- binding ... vitro. Abbreviations ADPRT, ADP-ribosyltransferase; BD, binding domain; ExoS, exoenzyme S; FAS, factor activating exoenzyme S; GAP, GTPase-activating protein; GEF, guanine exchange facto...

Ngày tải lên: 19/02/2014, 08:20

... data are in line with previous observations indicating that physiological activators of the p38 and PtdIns 3 -kinase pathways including thr ombin, dexamethasone, angiotensin II and prostaglandins ... required an intact cytoskeleton, and, at least in part, the upstream activation of PtdIns 3 -kinase. Inhibition of PtdIns 3 -kinase reduced both p38 and PKB/Akt phosphorylation i...

Ngày tải lên: 19/02/2014, 16:20

... EVALUATION OF MACHINE TRANSLATIONS 3 lations than they did the human translations. This measure shows a practice effect in the case of the ma- chine translations, though not for human translations. ... indicate that a good deal of information is available in machine translations, they are also consistent with the view that it is less readily available than in human tra...

Ngày tải lên: 19/02/2014, 19:20

... within a multigene family is regulated by transcription factor TFIIIB Akhila Parthasarthy and Karumathil P. Gopinathan Department of Microbiology and Cell Biology, Indian Institute of Science, Bangalore, ... with increasing concentrations of the unla- belled fragment (lanes 3 and 4, 10 and 100·, respectively); same fragment from which the TATATAA sequence was mutated to GATATCA (lan...

Ngày tải lên: 20/02/2014, 02:21

... linked to the phosphorylation of proteins such as mitochon- drial proteins, caspases, transcriptional factors and nuclear proteins. Phosphorylation of MAPK signaling pathways Many protein kinases ... downstream of caspase activation. Many kinases ⁄ phosphata- ses are involved in the control of cell survival and death, including the mitogen-activated protein kinase signal t...

Ngày tải lên: 14/02/2014, 22:20