Tài liệu Báo cáo khoa học: Biosynthesis of riboflavin Screening for an improved GTP cyclohydrolase II mutant pdf

... FEBS 4129 Biosynthesis of riboflavin Screening for an improved GTP cyclohydrolase II mutant Martin Lehmann 1 , Simone Degen 1 , Hans-Peter Hohmann 1 , Markus Wyss 1 , Adelbert Bacher 2 and Nicholas ... sig- nificant GTP cyclohydrolase II activity. The library of recombinant E. coli strains afforded a library of mutant proteins that was assayed for GTP cycl...

Ngày tải lên: 18/02/2014, 11:20

... (Institute of Medical Science, University of Tokyo, Japan) for providing pCAG-Cre; Dr Ikuo Yana (Institute of Medical Science, University of Tokyo, Japan) for help in per- forming endothelial tube formation ... problem. Therefore, the isolation and maintenance of murine endothelial cells from various developmental stages and locations is important for dissecting molecular and...

Ngày tải lên: 18/02/2014, 17:20

... main features of the UAM CorpusTool, software for human and semi-automatic annotation of text and images. The demonstration will show how to set up an annotation project, how to annotate text ... allows for partially overlap- ping segments, and embedding of segments. Annotated texts are stored using stand-off XML, one file per source text and layer. See Figure 4 for a sam...

Ngày tải lên: 20/02/2014, 09:20

... the effect of morphol- ogy on SMT quality focused on morphologically rich languages such as German (Nießen and Ney, 2004); Spanish, Catalan, and Serbian (Popovi´c 1 and Ney, 2004); and Czech (Goldwater ... (or lemma) of an Arabic word is not always an easy task and often requires the use of a morphological analyzer. One common example in Arabic nouns is Broken Plurals. For example...

Ngày tải lên: 20/02/2014, 11:21

... magnitude of that task. The servers contain the necessary information for many more words than the base system. This information can be extracted at development time, modified if appropriate (for ... object. Words other than verbs can have similar information. The semantics server contains such information for about 4300 words, mostly verbs; the verbs account for over 60% of...

Ngày tải lên: 20/02/2014, 18:20

... 168 a K m for 5-amino-6-ribitylamino-2,4(1H,3H)-pyrimidinedione. b K m for 3,4-dihydroxy-2-butanone 4-phosphate. c K d for riboflavin. d K i for riboflavin. Ó FEBS 2002 Biosynthesis of riboflavin ... case of the W27Y mutant, the absorption and CD spectra of the riboflavin/ enzyme complex differed substantially from those of the wild-type protein (Figs 3 and 4B). Whereas...

Ngày tải lên: 08/03/2014, 10:20

... E A of 63.7 kJÆmol )1 andanArrheniusconstantof A ¼ 2.9 · 10 8 s )1 (Fig. 2, Table 4). Sedimentation equilibrium analysis of M jannaschii pro- duced an approximate mass of 1.1 MDa suggesting an icosahedral ... jannaschii, Richard Feicht and Lars Schulte for skillfull assistance and Angelika Werner for help with the preparation of the manuscript. This work was supported by grant...

Ngày tải lên: 23/03/2014, 21:20

... thank H. Su- zuki and members of OLYMPUS Corporation for helpful discussion and much technical advice. This work was supported by grants from SENTAN, JST to K. Takeyasu and a Grant-in-Aid for ... l M free Ca 2+ and in the presence of 10 nM (B) and 1 lM ATP (C), respectively [scanning rate of 250 Hz, scan scale of 208 nm (y-axis direction in the FSAFM images), and z-scale of 40.0...

Ngày tải lên: 14/02/2014, 14:20

... Zhang GY, Zhang Y, Yang H & Yang LX (2007) [Expression of HMGB-1 and its extracellular release of cultured primary hepatic parenchymal cells and Kupffer cells induced by LPS]. Zhonghua Gan ... for at least 1 week. All animal procedures were approved by the Sungkyunkwan University Animal Care Committee and were performed in accordance with the guidelines of the National Institutes...

Ngày tải lên: 14/02/2014, 18:20

... our understanding of the importance of different parts of the molecule and their flexibility in the process of amy- loid fibril formation often results more from a struc- tural analysis of the amyloid ... solvent. There- fore, the solvation of backbone atoms is much larger for a conformers than for b conformers. Interaction with solvent thus compensates for the destabilization...

Ngày tải lên: 14/02/2014, 18:20